The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma

The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma

Abstract The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-r...

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Autores principales: Parichehr Maleki, Seyed Javad Mowla, Mohammad Taheri, Soudeh Ghafouri-Fard, Jamshid Raheb
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/96ee9276c2b64f6aa3ce54aa399c4af9
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spelling oai:doaj.org-article:96ee9276c2b64f6aa3ce54aa399c4af92021-12-02T12:11:28ZThe role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma10.1038/s41598-021-82892-z2045-2322https://doaj.org/article/96ee9276c2b64f6aa3ce54aa399c4af92021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82892-zhttps://doaj.org/toc/2045-2322Abstract The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation of LINK-A in A549 and Calu-3 cell lines as cellular models of non-small cell lung carcinoma (NSCLC). We used the RNA interference system to knock down LINK-A. LINK-A expression was significantly reduced by siRNA transfection in A549 and Calu-3 cell lines. LINK-A down-regulation significantly reduced cell viability, colony-forming ability and cell migration, as measured by MTT, colony formation and invasion assays. Finally, cell cycle analysis and Annexin-V/7AAD staining indicated that apoptosis was influenced by LINK-A silencing. Taken together, LINK-A can be proposed as an oncogene in NSCLC.Parichehr MalekiSeyed Javad MowlaMohammad TaheriSoudeh Ghafouri-FardJamshid RahebNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Parichehr Maleki
Seyed Javad Mowla
Mohammad Taheri
Soudeh Ghafouri-Fard
Jamshid Raheb
The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
description Abstract The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation of LINK-A in A549 and Calu-3 cell lines as cellular models of non-small cell lung carcinoma (NSCLC). We used the RNA interference system to knock down LINK-A. LINK-A expression was significantly reduced by siRNA transfection in A549 and Calu-3 cell lines. LINK-A down-regulation significantly reduced cell viability, colony-forming ability and cell migration, as measured by MTT, colony formation and invasion assays. Finally, cell cycle analysis and Annexin-V/7AAD staining indicated that apoptosis was influenced by LINK-A silencing. Taken together, LINK-A can be proposed as an oncogene in NSCLC.
format article
author Parichehr Maleki
Seyed Javad Mowla
Mohammad Taheri
Soudeh Ghafouri-Fard
Jamshid Raheb
author_facet Parichehr Maleki
Seyed Javad Mowla
Mohammad Taheri
Soudeh Ghafouri-Fard
Jamshid Raheb
author_sort Parichehr Maleki
title The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_short The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_full The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_fullStr The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_full_unstemmed The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_sort role of long intergenic non-coding rna for kinase activation (link-a) as an oncogene in non-small cell lung carcinoma
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/96ee9276c2b64f6aa3ce54aa399c4af9
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