Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions

Abstract The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniela Welcker, Cornelia Stein, Natalia Martins Feitosa, Joy Armistead, Jin-Li Zhang, Steffen Lütke, Andre Kleinridders, Jens C. Brüning, Sabine A. Eming, Gerhard Sengle, Anja Niehoff, Wilhelm Bloch, Matthias Hammerschmidt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96f15bdd865a41c29b23c156bb4759bc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:96f15bdd865a41c29b23c156bb4759bc
record_format	dspace
spelling	oai:doaj.org-article:96f15bdd865a41c29b23c156bb4759bc2021-12-02T17:41:17ZHemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions10.1038/s41598-021-96824-42045-2322https://doaj.org/article/96f15bdd865a41c29b23c156bb4759bc2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96824-4https://doaj.org/toc/2045-2322Abstract The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Performing in situ hybridization and immunofluorescence analyses, we found that mouse Hmcn1 and Hmcn2 show a complementary distribution throughout different tissues and developmental stages. In postnatal dermal–epidermal junctions (DEJ) and myotendinous junctions (MTJ), Hmcn1 is primarily produced by mesenchymal cells (fibroblasts, tenocytes), Hmcn2 by cells of epithelial origin (keratinocytes, myocytes). Hmcn1 −/− mice are viable and show no overt phenotypes in tissue tensile strength and locomotion tests. However, transmission electron microscopy revealed ultrastructural basement membrane (BM) alterations at the DEJ and MTJ of Hmcn1 −/− mice, pointing to a thus far unknown role of Hmcn1 for BM and connective tissue boundary integrity.Daniela WelckerCornelia SteinNatalia Martins FeitosaJoy ArmisteadJin-Li ZhangSteffen LütkeAndre KleinriddersJens C. BrüningSabine A. EmingGerhard SengleAnja NiehoffWilhelm BlochMatthias HammerschmidtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Welcker Cornelia Stein Natalia Martins Feitosa Joy Armistead Jin-Li Zhang Steffen Lütke Andre Kleinridders Jens C. Brüning Sabine A. Eming Gerhard Sengle Anja Niehoff Wilhelm Bloch Matthias Hammerschmidt Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
description	Abstract The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Performing in situ hybridization and immunofluorescence analyses, we found that mouse Hmcn1 and Hmcn2 show a complementary distribution throughout different tissues and developmental stages. In postnatal dermal–epidermal junctions (DEJ) and myotendinous junctions (MTJ), Hmcn1 is primarily produced by mesenchymal cells (fibroblasts, tenocytes), Hmcn2 by cells of epithelial origin (keratinocytes, myocytes). Hmcn1 −/− mice are viable and show no overt phenotypes in tissue tensile strength and locomotion tests. However, transmission electron microscopy revealed ultrastructural basement membrane (BM) alterations at the DEJ and MTJ of Hmcn1 −/− mice, pointing to a thus far unknown role of Hmcn1 for BM and connective tissue boundary integrity.
format	article
author	Daniela Welcker Cornelia Stein Natalia Martins Feitosa Joy Armistead Jin-Li Zhang Steffen Lütke Andre Kleinridders Jens C. Brüning Sabine A. Eming Gerhard Sengle Anja Niehoff Wilhelm Bloch Matthias Hammerschmidt
author_facet	Daniela Welcker Cornelia Stein Natalia Martins Feitosa Joy Armistead Jin-Li Zhang Steffen Lütke Andre Kleinridders Jens C. Brüning Sabine A. Eming Gerhard Sengle Anja Niehoff Wilhelm Bloch Matthias Hammerschmidt
author_sort	Daniela Welcker
title	Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
title_short	Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
title_full	Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
title_fullStr	Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
title_full_unstemmed	Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
title_sort	hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/96f15bdd865a41c29b23c156bb4759bc
work_keys_str_mv	AT danielawelcker hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT corneliastein hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT nataliamartinsfeitosa hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT joyarmistead hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT jinlizhang hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT steffenlutke hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT andrekleinridders hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT jenscbruning hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT sabineaeming hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT gerhardsengle hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT anjaniehoff hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT wilhelmbloch hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions AT matthiashammerschmidt hemicentin1isanessentialextracellularmatrixcomponentofthedermalepidermalandmyotendinousjunctions
_version_	1718379718489669632

Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions

Ejemplares similares