Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution

Abstract Amyloid β (Aβ) oligomers may play a decisive role in Alzheimer’s disease related neurodegeneration, but their structural properties are poorly understood. In this report, sedimentation velocity centrifugation, small angle neutron scattering (SANS) and molecular modelling were used to identi...

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Autores principales:	Martin Wolff, Bo Zhang-Haagen, Christina Decker, Bogdan Barz, Mario Schneider, Ralf Biehl, Aurel Radulescu, Birgit Strodel, Dieter Willbold, Luitgard Nagel-Steger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96f163f4a72e4c548032532f4e1748db
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spelling	oai:doaj.org-article:96f163f4a72e4c548032532f4e1748db2021-12-02T12:32:30ZAβ42 pentamers/hexamers are the smallest detectable oligomers in solution10.1038/s41598-017-02370-32045-2322https://doaj.org/article/96f163f4a72e4c548032532f4e1748db2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02370-3https://doaj.org/toc/2045-2322Abstract Amyloid β (Aβ) oligomers may play a decisive role in Alzheimer’s disease related neurodegeneration, but their structural properties are poorly understood. In this report, sedimentation velocity centrifugation, small angle neutron scattering (SANS) and molecular modelling were used to identify the small oligomeric species formed by the 42 amino acid residue long isoform of Aβ (Aβ42) in solution, characterized by a sedimentation coefficient of 2.56 S, and a radius of gyration between 2 and 4 nm. The measured sedimentation coefficient is in close agreement with the sedimentation coefficient calculated for Aβ42 hexamers using MD simulations at µM concentration. To the best of our knowledge this is the first report detailing the Aβ42 oligomeric species by SANS measurements. Our results demonstrate that the smallest detectable species in solution are penta- to hexamers. No evidences for the presence of dimers, trimers or tetramers were found, although the existence of those Aβ42 oligomers at measurable quantities had been reported frequently.Martin WolffBo Zhang-HaagenChristina DeckerBogdan BarzMario SchneiderRalf BiehlAurel RadulescuBirgit StrodelDieter WillboldLuitgard Nagel-StegerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Wolff Bo Zhang-Haagen Christina Decker Bogdan Barz Mario Schneider Ralf Biehl Aurel Radulescu Birgit Strodel Dieter Willbold Luitgard Nagel-Steger Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
description	Abstract Amyloid β (Aβ) oligomers may play a decisive role in Alzheimer’s disease related neurodegeneration, but their structural properties are poorly understood. In this report, sedimentation velocity centrifugation, small angle neutron scattering (SANS) and molecular modelling were used to identify the small oligomeric species formed by the 42 amino acid residue long isoform of Aβ (Aβ42) in solution, characterized by a sedimentation coefficient of 2.56 S, and a radius of gyration between 2 and 4 nm. The measured sedimentation coefficient is in close agreement with the sedimentation coefficient calculated for Aβ42 hexamers using MD simulations at µM concentration. To the best of our knowledge this is the first report detailing the Aβ42 oligomeric species by SANS measurements. Our results demonstrate that the smallest detectable species in solution are penta- to hexamers. No evidences for the presence of dimers, trimers or tetramers were found, although the existence of those Aβ42 oligomers at measurable quantities had been reported frequently.
format	article
author	Martin Wolff Bo Zhang-Haagen Christina Decker Bogdan Barz Mario Schneider Ralf Biehl Aurel Radulescu Birgit Strodel Dieter Willbold Luitgard Nagel-Steger
author_facet	Martin Wolff Bo Zhang-Haagen Christina Decker Bogdan Barz Mario Schneider Ralf Biehl Aurel Radulescu Birgit Strodel Dieter Willbold Luitgard Nagel-Steger
author_sort	Martin Wolff
title	Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
title_short	Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
title_full	Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
title_fullStr	Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
title_full_unstemmed	Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
title_sort	aβ42 pentamers/hexamers are the smallest detectable oligomers in solution
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/96f163f4a72e4c548032532f4e1748db
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Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution

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