Clonogenicity: holoclones and meroclones contain stem cells.

When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones. These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively. More recently, this approach has been extended to can...

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Autores principales:	Charlotte M Beaver, Aamir Ahmed, John R Masters
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96f7e56360ad4704ac5b29ed49317ce7
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spelling	oai:doaj.org-article:96f7e56360ad4704ac5b29ed49317ce72021-11-18T08:30:51ZClonogenicity: holoclones and meroclones contain stem cells.1932-620310.1371/journal.pone.0089834https://doaj.org/article/96f7e56360ad4704ac5b29ed49317ce72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587067/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones. These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively. More recently, this approach has been extended to cancer cell lines. However, we observed that meroclones from the prostate cancer cell line DU145 produce holoclones, a paradoxical observation as meroclones are thought to be derived from transit-amplifying cells. The purpose of this study was to confirm this observation and determine if both holoclones and meroclones from cancer cell lines contain stem cells. We demonstrated that both holoclones and meroclones can be serially passaged indefinitely, are highly proliferative, can self-renew to form spheres, are serially tumorigenic and express stem cell markers. This study demonstrates that the major difference between holoclones and meroclones derived from a cancer cell line is the proportion of stem cells within each colony, not the presence or absence of stem cells. These findings may reflect the properties of cancer as opposed to normal cells, perhaps indicating that the hierarchy of stem cells is more extensive in cancer.Charlotte M BeaverAamir AhmedJohn R MastersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89834 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlotte M Beaver Aamir Ahmed John R Masters Clonogenicity: holoclones and meroclones contain stem cells.
description	When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones. These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively. More recently, this approach has been extended to cancer cell lines. However, we observed that meroclones from the prostate cancer cell line DU145 produce holoclones, a paradoxical observation as meroclones are thought to be derived from transit-amplifying cells. The purpose of this study was to confirm this observation and determine if both holoclones and meroclones from cancer cell lines contain stem cells. We demonstrated that both holoclones and meroclones can be serially passaged indefinitely, are highly proliferative, can self-renew to form spheres, are serially tumorigenic and express stem cell markers. This study demonstrates that the major difference between holoclones and meroclones derived from a cancer cell line is the proportion of stem cells within each colony, not the presence or absence of stem cells. These findings may reflect the properties of cancer as opposed to normal cells, perhaps indicating that the hierarchy of stem cells is more extensive in cancer.
format	article
author	Charlotte M Beaver Aamir Ahmed John R Masters
author_facet	Charlotte M Beaver Aamir Ahmed John R Masters
author_sort	Charlotte M Beaver
title	Clonogenicity: holoclones and meroclones contain stem cells.
title_short	Clonogenicity: holoclones and meroclones contain stem cells.
title_full	Clonogenicity: holoclones and meroclones contain stem cells.
title_fullStr	Clonogenicity: holoclones and meroclones contain stem cells.
title_full_unstemmed	Clonogenicity: holoclones and meroclones contain stem cells.
title_sort	clonogenicity: holoclones and meroclones contain stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/96f7e56360ad4704ac5b29ed49317ce7
work_keys_str_mv	AT charlottembeaver clonogenicityholoclonesandmeroclonescontainstemcells AT aamirahmed clonogenicityholoclonesandmeroclonescontainstemcells AT johnrmasters clonogenicityholoclonesandmeroclonescontainstemcells
_version_	1718421698057863168

Clonogenicity: holoclones and meroclones contain stem cells.

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