The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts

Abstract The miR-183 cluster, which is comprised of paralogous miRs-183, -96 and -182, is overexpressed in many cancers, including prostate adenocarcinoma (PCa). Prior studies showed that overexpression of individual pre-miRs-182, -96 and -183 in prostate cells decreased zinc import, which is a char...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shweta Dambal, Bethany Baumann, Tara McCray, LaTanya Williams, Zachary Richards, Ryan Deaton, Gail S. Prins, Larisa Nonn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96ffe93432e54d89a630744093f95664
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:96ffe93432e54d89a630744093f95664
record_format	dspace
spelling	oai:doaj.org-article:96ffe93432e54d89a630744093f956642021-12-02T11:40:13ZThe miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts10.1038/s41598-017-07979-y2045-2322https://doaj.org/article/96ffe93432e54d89a630744093f956642017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07979-yhttps://doaj.org/toc/2045-2322Abstract The miR-183 cluster, which is comprised of paralogous miRs-183, -96 and -182, is overexpressed in many cancers, including prostate adenocarcinoma (PCa). Prior studies showed that overexpression of individual pre-miRs-182, -96 and -183 in prostate cells decreased zinc import, which is a characteristic feature of PCa tumours. Zinc is concentrated in healthy prostate 10-fold higher than any other tissue, and an >80% decrease in zinc is observed in PCa specimens. Here, we studied the effect of overexpression of the entire 4.8 kb miR-183 family cluster, including the intergenic region which contains highly conserved genomic regions, in prostate cells. This resulted in overexpression of mature miR-183 family miRs at levels that mimic cancer-related changes. Overexpression of the miR-183 cluster reduced zinc transporter and intracellular zinc levels in benign prostate cells, PCa xenografts and fresh prostate epithelial organoids. Microarray analysis of miR-183 family cluster overexpression in prostate cells showed an enrichment for cancer-related pathways including adhesion, migration and wound healing. An active secondary transcription start site was identified within the intergenic region of the miR-183 cluster, which may regulate expression of miR-182. Taken together, this study shows that physiologically relevant expression of the miR-183 family regulates zinc levels and carcinogenic pathways in prostate cells.Shweta DambalBethany BaumannTara McCrayLaTanya WilliamsZachary RichardsRyan DeatonGail S. PrinsLarisa NonnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shweta Dambal Bethany Baumann Tara McCray LaTanya Williams Zachary Richards Ryan Deaton Gail S. Prins Larisa Nonn The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
description	Abstract The miR-183 cluster, which is comprised of paralogous miRs-183, -96 and -182, is overexpressed in many cancers, including prostate adenocarcinoma (PCa). Prior studies showed that overexpression of individual pre-miRs-182, -96 and -183 in prostate cells decreased zinc import, which is a characteristic feature of PCa tumours. Zinc is concentrated in healthy prostate 10-fold higher than any other tissue, and an >80% decrease in zinc is observed in PCa specimens. Here, we studied the effect of overexpression of the entire 4.8 kb miR-183 family cluster, including the intergenic region which contains highly conserved genomic regions, in prostate cells. This resulted in overexpression of mature miR-183 family miRs at levels that mimic cancer-related changes. Overexpression of the miR-183 cluster reduced zinc transporter and intracellular zinc levels in benign prostate cells, PCa xenografts and fresh prostate epithelial organoids. Microarray analysis of miR-183 family cluster overexpression in prostate cells showed an enrichment for cancer-related pathways including adhesion, migration and wound healing. An active secondary transcription start site was identified within the intergenic region of the miR-183 cluster, which may regulate expression of miR-182. Taken together, this study shows that physiologically relevant expression of the miR-183 family regulates zinc levels and carcinogenic pathways in prostate cells.
format	article
author	Shweta Dambal Bethany Baumann Tara McCray LaTanya Williams Zachary Richards Ryan Deaton Gail S. Prins Larisa Nonn
author_facet	Shweta Dambal Bethany Baumann Tara McCray LaTanya Williams Zachary Richards Ryan Deaton Gail S. Prins Larisa Nonn
author_sort	Shweta Dambal
title	The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
title_short	The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
title_full	The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
title_fullStr	The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
title_full_unstemmed	The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
title_sort	mir-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/96ffe93432e54d89a630744093f95664
work_keys_str_mv	AT shwetadambal themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT bethanybaumann themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT taramccray themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT latanyawilliams themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT zacharyrichards themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT ryandeaton themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT gailsprins themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT larisanonn themir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT shwetadambal mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT bethanybaumann mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT taramccray mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT latanyawilliams mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT zacharyrichards mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT ryandeaton mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT gailsprins mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts AT larisanonn mir183familyclusteralterszinchomeostasisinbenignprostatecellsorganoidsandprostatecancerxenografts
_version_	1718395715317661696

The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts

Ejemplares similares