Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel

Abstract Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engine...

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Autores principales:	Ilaria Sanzari, Mauro Callisti, Antonio De Grazia, Daniel J. Evans, Tomas Polcar, Themistoklis Prodromakis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9708a87e10894147856f35193dd664e0
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spelling	oai:doaj.org-article:9708a87e10894147856f35193dd664e02021-12-02T16:06:35ZParylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel10.1038/s41598-017-05434-62045-2322https://doaj.org/article/9708a87e10894147856f35193dd664e02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05434-6https://doaj.org/toc/2045-2322Abstract Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm. Polydimethylsiloxane (PDMS) and Polyacrylamide (PAm) hydrogel have been chosen as soft polymers to be moulded. Thanks to their chemical and physical properties PDMS and PAm hydrogel mimic the extracellular matrix (ECM). PDMS was spin coated on micropatterned Parylene C obtaining composite substrates with 460 nm and 1.15 µm high grooves. The Young’s modulus of the composite Parylene C/PDMS was evaluated and it was found to be almost half when compared to PDMS. PAm hydrogel was also printed using collagen coated micro-grooved Parylene C. Optical micrographs and fluorescence analysis show the successful topographic and protein pattern transfer on the hydrogel.Ilaria SanzariMauro CallistiAntonio De GraziaDaniel J. EvansTomas PolcarThemistoklis ProdromakisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ilaria Sanzari Mauro Callisti Antonio De Grazia Daniel J. Evans Tomas Polcar Themistoklis Prodromakis Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
description	Abstract Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm. Polydimethylsiloxane (PDMS) and Polyacrylamide (PAm) hydrogel have been chosen as soft polymers to be moulded. Thanks to their chemical and physical properties PDMS and PAm hydrogel mimic the extracellular matrix (ECM). PDMS was spin coated on micropatterned Parylene C obtaining composite substrates with 460 nm and 1.15 µm high grooves. The Young’s modulus of the composite Parylene C/PDMS was evaluated and it was found to be almost half when compared to PDMS. PAm hydrogel was also printed using collagen coated micro-grooved Parylene C. Optical micrographs and fluorescence analysis show the successful topographic and protein pattern transfer on the hydrogel.
format	article
author	Ilaria Sanzari Mauro Callisti Antonio De Grazia Daniel J. Evans Tomas Polcar Themistoklis Prodromakis
author_facet	Ilaria Sanzari Mauro Callisti Antonio De Grazia Daniel J. Evans Tomas Polcar Themistoklis Prodromakis
author_sort	Ilaria Sanzari
title	Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
title_short	Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
title_full	Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
title_fullStr	Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
title_full_unstemmed	Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
title_sort	parylene c topographic micropattern as a template for patterning pdms and polyacrylamide hydrogel
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9708a87e10894147856f35193dd664e0
work_keys_str_mv	AT ilariasanzari parylenectopographicmicropatternasatemplateforpatterningpdmsandpolyacrylamidehydrogel AT maurocallisti parylenectopographicmicropatternasatemplateforpatterningpdmsandpolyacrylamidehydrogel AT antoniodegrazia parylenectopographicmicropatternasatemplateforpatterningpdmsandpolyacrylamidehydrogel AT danieljevans parylenectopographicmicropatternasatemplateforpatterningpdmsandpolyacrylamidehydrogel AT tomaspolcar parylenectopographicmicropatternasatemplateforpatterningpdmsandpolyacrylamidehydrogel AT themistoklisprodromakis parylenectopographicmicropatternasatemplateforpatterningpdmsandpolyacrylamidehydrogel
_version_	1718384976627499008

Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel

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