Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia

A total of 415 cheese samples produced with raw or cooked milk collected from local markets were analysed for the presence of coagulase-positive staphylococci. In 85 (20.48%) samples the presence of coagulase positive staphylococci was detected. The ELFA technique VIDAS SET2 (BioMerieux, France) was...

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Autores principales:	Radoslava Savić Radovanović, Nemanja Zdravković, Branko Velebit
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2020
Materias:	coagulase-positive staphylococci enterotoxins raw milk cheeses Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/9708d72512d14d1c80880f7993aa89e8
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spelling	oai:doaj.org-article:9708d72512d14d1c80880f7993aa89e82021-11-17T21:27:53ZOccurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia1820-744810.2478/acve-2020-0017https://doaj.org/article/9708d72512d14d1c80880f7993aa89e82020-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2020-0017https://doaj.org/toc/1820-7448A total of 415 cheese samples produced with raw or cooked milk collected from local markets were analysed for the presence of coagulase-positive staphylococci. In 85 (20.48%) samples the presence of coagulase positive staphylococci was detected. The ELFA technique VIDAS SET2 (BioMerieux, France) was used for testing coagulase-positive staphylococci strains to produce classical enterotoxins (SEA, SEB, SEC, SED, SEE), and to determine the enterotoxins in cheese samples. The number of coagulase-positive staphylococci in cheese samples ranged from 1-5.79 log CFU g-1. Out of 85 coagulase-positive strains 26 (30.59%) produced enterotoxins. The presence of genes for the synthesis of staphylococcal enterotoxins (SE) in the obtained extracts of DNA from 26 enterotoxigenic strains was detected by conventional multiplex PCR technique (for genes sea and seb) i.e. the Real-Time PCR technique for genes sec, sed and see. In all 26 strains of coagulase-positive staphylococci (originating from cheeses produced from raw or cooked milk, which were enterotoxin producers) sea was present, and in 24 strains in addition to sea gene, seb was detected. None of the isolates possessed genes for the synthesis of enterotoxin C (SEC), D (SED) and E (SEE). Out of 26 tested cheese samples positive for enterotoxigenic coagulase-positive staphylococci, enterotoxin was detected in 2 (7.69%) samples of sweet-coagulating cheese, in which the number of enterotoxigenic coagulase-positive staphylococci exceeded 5 log CFU g-1. In sweet-coagulating cheeses in which the number of coagulase-positive staphylococci exceeds 5 log CFU g-1 and the pH value was higher than 5.0, enterotoxins may be present in amounts sufficient to cause intoxication.Radoslava Savić RadovanovićNemanja ZdravkovićBranko VelebitSciendoarticlecoagulase-positive staphylococcienterotoxinsraw milkcheesesVeterinary medicineSF600-1100ENActa Veterinaria, Vol 70, Iss 2, Pp 238-254 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	coagulase-positive staphylococci enterotoxins raw milk cheeses Veterinary medicine SF600-1100
spellingShingle	coagulase-positive staphylococci enterotoxins raw milk cheeses Veterinary medicine SF600-1100 Radoslava Savić Radovanović Nemanja Zdravković Branko Velebit Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia
description	A total of 415 cheese samples produced with raw or cooked milk collected from local markets were analysed for the presence of coagulase-positive staphylococci. In 85 (20.48%) samples the presence of coagulase positive staphylococci was detected. The ELFA technique VIDAS SET2 (BioMerieux, France) was used for testing coagulase-positive staphylococci strains to produce classical enterotoxins (SEA, SEB, SEC, SED, SEE), and to determine the enterotoxins in cheese samples. The number of coagulase-positive staphylococci in cheese samples ranged from 1-5.79 log CFU g-1. Out of 85 coagulase-positive strains 26 (30.59%) produced enterotoxins. The presence of genes for the synthesis of staphylococcal enterotoxins (SE) in the obtained extracts of DNA from 26 enterotoxigenic strains was detected by conventional multiplex PCR technique (for genes sea and seb) i.e. the Real-Time PCR technique for genes sec, sed and see. In all 26 strains of coagulase-positive staphylococci (originating from cheeses produced from raw or cooked milk, which were enterotoxin producers) sea was present, and in 24 strains in addition to sea gene, seb was detected. None of the isolates possessed genes for the synthesis of enterotoxin C (SEC), D (SED) and E (SEE). Out of 26 tested cheese samples positive for enterotoxigenic coagulase-positive staphylococci, enterotoxin was detected in 2 (7.69%) samples of sweet-coagulating cheese, in which the number of enterotoxigenic coagulase-positive staphylococci exceeded 5 log CFU g-1. In sweet-coagulating cheeses in which the number of coagulase-positive staphylococci exceeds 5 log CFU g-1 and the pH value was higher than 5.0, enterotoxins may be present in amounts sufficient to cause intoxication.
format	article
author	Radoslava Savić Radovanović Nemanja Zdravković Branko Velebit
author_facet	Radoslava Savić Radovanović Nemanja Zdravković Branko Velebit
author_sort	Radoslava Savić Radovanović
title	Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia
title_short	Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia
title_full	Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia
title_fullStr	Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia
title_full_unstemmed	Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia
title_sort	occurrence and characterization of enterotoxigenic staphylococci isolated from soft cheeses in serbia
publisher	Sciendo
publishDate	2020
url	https://doaj.org/article/9708d72512d14d1c80880f7993aa89e8
work_keys_str_mv	AT radoslavasavicradovanovic occurrenceandcharacterizationofenterotoxigenicstaphylococciisolatedfromsoftcheesesinserbia AT nemanjazdravkovic occurrenceandcharacterizationofenterotoxigenicstaphylococciisolatedfromsoftcheesesinserbia AT brankovelebit occurrenceandcharacterizationofenterotoxigenicstaphylococciisolatedfromsoftcheesesinserbia
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Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia

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