Virtual-freezing fluorescence imaging flow cytometry

High throughput imaging flow cytometry suffers from trade-offs between throughput, sensitivity and spatial resolution. Here the authors introduce a method to virtually freeze cells in the image acquisition window to enable 1000 times longer signal integration time and improve signal-to-noise ratio.

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Autores principales:	Hideharu Mikami, Makoto Kawaguchi, Chun-Jung Huang, Hiroki Matsumura, Takeaki Sugimura, Kangrui Huang, Cheng Lei, Shunnosuke Ueno, Taichi Miura, Takuro Ito, Kazumichi Nagasawa, Takanori Maeno, Hiroshi Watarai, Mai Yamagishi, Sotaro Uemura, Shinsuke Ohnuki, Yoshikazu Ohya, Hiromi Kurokawa, Satoshi Matsusaka, Chia-Wei Sun, Yasuyuki Ozeki, Keisuke Goda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/970eb547b4534e9b8248827dc2f21c9b
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spelling	oai:doaj.org-article:970eb547b4534e9b8248827dc2f21c9b2021-12-02T17:33:12ZVirtual-freezing fluorescence imaging flow cytometry10.1038/s41467-020-14929-22041-1723https://doaj.org/article/970eb547b4534e9b8248827dc2f21c9b2020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14929-2https://doaj.org/toc/2041-1723High throughput imaging flow cytometry suffers from trade-offs between throughput, sensitivity and spatial resolution. Here the authors introduce a method to virtually freeze cells in the image acquisition window to enable 1000 times longer signal integration time and improve signal-to-noise ratio.Hideharu MikamiMakoto KawaguchiChun-Jung HuangHiroki MatsumuraTakeaki SugimuraKangrui HuangCheng LeiShunnosuke UenoTaichi MiuraTakuro ItoKazumichi NagasawaTakanori MaenoHiroshi WataraiMai YamagishiSotaro UemuraShinsuke OhnukiYoshikazu OhyaHiromi KurokawaSatoshi MatsusakaChia-Wei SunYasuyuki OzekiKeisuke GodaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hideharu Mikami Makoto Kawaguchi Chun-Jung Huang Hiroki Matsumura Takeaki Sugimura Kangrui Huang Cheng Lei Shunnosuke Ueno Taichi Miura Takuro Ito Kazumichi Nagasawa Takanori Maeno Hiroshi Watarai Mai Yamagishi Sotaro Uemura Shinsuke Ohnuki Yoshikazu Ohya Hiromi Kurokawa Satoshi Matsusaka Chia-Wei Sun Yasuyuki Ozeki Keisuke Goda Virtual-freezing fluorescence imaging flow cytometry
description	High throughput imaging flow cytometry suffers from trade-offs between throughput, sensitivity and spatial resolution. Here the authors introduce a method to virtually freeze cells in the image acquisition window to enable 1000 times longer signal integration time and improve signal-to-noise ratio.
format	article
author	Hideharu Mikami Makoto Kawaguchi Chun-Jung Huang Hiroki Matsumura Takeaki Sugimura Kangrui Huang Cheng Lei Shunnosuke Ueno Taichi Miura Takuro Ito Kazumichi Nagasawa Takanori Maeno Hiroshi Watarai Mai Yamagishi Sotaro Uemura Shinsuke Ohnuki Yoshikazu Ohya Hiromi Kurokawa Satoshi Matsusaka Chia-Wei Sun Yasuyuki Ozeki Keisuke Goda
author_facet	Hideharu Mikami Makoto Kawaguchi Chun-Jung Huang Hiroki Matsumura Takeaki Sugimura Kangrui Huang Cheng Lei Shunnosuke Ueno Taichi Miura Takuro Ito Kazumichi Nagasawa Takanori Maeno Hiroshi Watarai Mai Yamagishi Sotaro Uemura Shinsuke Ohnuki Yoshikazu Ohya Hiromi Kurokawa Satoshi Matsusaka Chia-Wei Sun Yasuyuki Ozeki Keisuke Goda
author_sort	Hideharu Mikami
title	Virtual-freezing fluorescence imaging flow cytometry
title_short	Virtual-freezing fluorescence imaging flow cytometry
title_full	Virtual-freezing fluorescence imaging flow cytometry
title_fullStr	Virtual-freezing fluorescence imaging flow cytometry
title_full_unstemmed	Virtual-freezing fluorescence imaging flow cytometry
title_sort	virtual-freezing fluorescence imaging flow cytometry
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/970eb547b4534e9b8248827dc2f21c9b
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Virtual-freezing fluorescence imaging flow cytometry

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