Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates

ABSTRACT Although mutation drives evolution over long and short terms, measuring and comparing mutation rates accurately have been particularly difficult. This is especially true when mutations lead to an alteration in fitness. E. Shor, J. Schuyler, and D. S. Perlin (https://doi.org/10.1128/mBio.001...

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Autores principales:	Kylie J. Boyce, Alexander Idnurm
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	CRISPR-Cas9 FACS GFP MSH2 antifungal drug resistance fluctuation test Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/971678cc1851430aafd9bfe69e7fbdfe
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spelling	oai:doaj.org-article:971678cc1851430aafd9bfe69e7fbdfe2021-11-15T15:55:26ZLighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates10.1128/mBio.00740-192150-7511https://doaj.org/article/971678cc1851430aafd9bfe69e7fbdfe2019-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00740-19https://doaj.org/toc/2150-7511ABSTRACT Although mutation drives evolution over long and short terms, measuring and comparing mutation rates accurately have been particularly difficult. This is especially true when mutations lead to an alteration in fitness. E. Shor, J. Schuyler, and D. S. Perlin (https://doi.org/10.1128/mBio.00120-19) present a new method to compare mutation rates across fungal strains and under different growth conditions: they employ the green fluorescent protein (GFP) as the reporter and count mutations using fluorescence-activated cell sorting (FACS). The estimates of mutation rates using the GFP-FACS approach are similar to those calculated with other reporters, and the method was used to assess if different alleles of the mismatch repair pathway gene MSH2 impact the mutation rates in the human pathogen Candida glabrata. The approach could be extended to other microbes and applications, opening the way for a better understanding of how mutation rates have impacted speciation and the emergence of antimicrobial resistance.Kylie J. BoyceAlexander IdnurmAmerican Society for MicrobiologyarticleCRISPR-Cas9FACSGFPMSH2antifungal drug resistancefluctuation testMicrobiologyQR1-502ENmBio, Vol 10, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR-Cas9 FACS GFP MSH2 antifungal drug resistance fluctuation test Microbiology QR1-502
spellingShingle	CRISPR-Cas9 FACS GFP MSH2 antifungal drug resistance fluctuation test Microbiology QR1-502 Kylie J. Boyce Alexander Idnurm Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates
description	ABSTRACT Although mutation drives evolution over long and short terms, measuring and comparing mutation rates accurately have been particularly difficult. This is especially true when mutations lead to an alteration in fitness. E. Shor, J. Schuyler, and D. S. Perlin (https://doi.org/10.1128/mBio.00120-19) present a new method to compare mutation rates across fungal strains and under different growth conditions: they employ the green fluorescent protein (GFP) as the reporter and count mutations using fluorescence-activated cell sorting (FACS). The estimates of mutation rates using the GFP-FACS approach are similar to those calculated with other reporters, and the method was used to assess if different alleles of the mismatch repair pathway gene MSH2 impact the mutation rates in the human pathogen Candida glabrata. The approach could be extended to other microbes and applications, opening the way for a better understanding of how mutation rates have impacted speciation and the emergence of antimicrobial resistance.
format	article
author	Kylie J. Boyce Alexander Idnurm
author_facet	Kylie J. Boyce Alexander Idnurm
author_sort	Kylie J. Boyce
title	Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates
title_short	Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates
title_full	Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates
title_fullStr	Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates
title_full_unstemmed	Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates
title_sort	lighting up mutation: a new unbiased system for the measurement of microbial mutation rates
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/971678cc1851430aafd9bfe69e7fbdfe
work_keys_str_mv	AT kyliejboyce lightingupmutationanewunbiasedsystemforthemeasurementofmicrobialmutationrates AT alexanderidnurm lightingupmutationanewunbiasedsystemforthemeasurementofmicrobialmutationrates
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Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates

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