The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice

Abstract Subchondral bone degeneration is the main pathological change during temporomandibular joint (TMJ) osteoarthritis (OA) development. Netrin-1, an axon-guiding factor, might play roles in OA development and pain. The purpose of this study was to investigate the expression of Netrin-1 in TMJ O...

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Autores principales:	Mian Xiao, Zhihui Hu, Henghua Jiang, Cheng Li, Huilin Guo, Wei Fang, Xing Long
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9717152f4baa476ab456221a1025ca92
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spelling	oai:doaj.org-article:9717152f4baa476ab456221a1025ca922021-12-02T18:49:16ZThe expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice10.1038/s41598-021-95251-92045-2322https://doaj.org/article/9717152f4baa476ab456221a1025ca922021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95251-9https://doaj.org/toc/2045-2322Abstract Subchondral bone degeneration is the main pathological change during temporomandibular joint (TMJ) osteoarthritis (OA) development. Netrin-1, an axon-guiding factor, might play roles in OA development and pain. The purpose of this study was to investigate the expression of Netrin-1 in TMJ OA and its possible role in the progression of TMJ OA and pain. The synovial fluids of temporomandibular joint disorders (TMDs) patients were collected for Netrin-1 by enzyme linked immunosorbent assay (ELISA). TMJ OA model was built by MIA joint injection, and then the von Frey test, hematoxylin & eosin (H&E) staining, toluidine blue (TB) staining, immunohistochemical (IHC) staining and micro-CT were performed. After induction of osteoclast differentiation of raw264.7 cells, immunofluorescence (IF) was used to detect the Netrin-1 and its receptors on osteoclast membrane. The concentration of Netrin-1 increased in the synovial fluid of TMJ OA patients. After MIA injection to TMJ, the head withdrawal threshold (HWT) was significantly decreased. Microscopically, the structural disorder of subchondral bone was the most obvious at the 2nd week after MIA injection. In addition, Netrin-1 expression increased in the subchondral bone at the 2nd week after MIA injection. In vitro, the expressions of Netrin-1 and its receptor Unc5B were upregulated on the osteoclast membrane. Netrin-1 might be an important regulator during bone degeneration and pain in the process of TMJ OA.Mian XiaoZhihui HuHenghua JiangCheng LiHuilin GuoWei FangXing LongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mian Xiao Zhihui Hu Henghua Jiang Cheng Li Huilin Guo Wei Fang Xing Long The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
description	Abstract Subchondral bone degeneration is the main pathological change during temporomandibular joint (TMJ) osteoarthritis (OA) development. Netrin-1, an axon-guiding factor, might play roles in OA development and pain. The purpose of this study was to investigate the expression of Netrin-1 in TMJ OA and its possible role in the progression of TMJ OA and pain. The synovial fluids of temporomandibular joint disorders (TMDs) patients were collected for Netrin-1 by enzyme linked immunosorbent assay (ELISA). TMJ OA model was built by MIA joint injection, and then the von Frey test, hematoxylin & eosin (H&E) staining, toluidine blue (TB) staining, immunohistochemical (IHC) staining and micro-CT were performed. After induction of osteoclast differentiation of raw264.7 cells, immunofluorescence (IF) was used to detect the Netrin-1 and its receptors on osteoclast membrane. The concentration of Netrin-1 increased in the synovial fluid of TMJ OA patients. After MIA injection to TMJ, the head withdrawal threshold (HWT) was significantly decreased. Microscopically, the structural disorder of subchondral bone was the most obvious at the 2nd week after MIA injection. In addition, Netrin-1 expression increased in the subchondral bone at the 2nd week after MIA injection. In vitro, the expressions of Netrin-1 and its receptor Unc5B were upregulated on the osteoclast membrane. Netrin-1 might be an important regulator during bone degeneration and pain in the process of TMJ OA.
format	article
author	Mian Xiao Zhihui Hu Henghua Jiang Cheng Li Huilin Guo Wei Fang Xing Long
author_facet	Mian Xiao Zhihui Hu Henghua Jiang Cheng Li Huilin Guo Wei Fang Xing Long
author_sort	Mian Xiao
title	The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
title_short	The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
title_full	The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
title_fullStr	The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
title_full_unstemmed	The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
title_sort	expression of netrin-1 in the mia-induced osteoarthritic temporomandibular joint in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9717152f4baa476ab456221a1025ca92
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The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice

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