A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues

Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cel...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Haruko Saiki, Yuko Okano, Taro Yasuma, Masaaki Toda, Atsuro Takeshita, Ahmed M. Abdel-Hamid, Valeria Fridman D’Alessandro, Tatsuki Tsuruga, Corina N. D’Alessandro-Gabazza, Kan Katayama, Masahiko Sugimoto, Hajime Fujimoto, Keiichi Yamanaka, Tetsu Kobayashi, Isaac Cann, Esteban C. Gabazza
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	corisin apoptosis organ fibrosis parenchymal cells different tissue Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/971803f952d24a18bd9b34477a1ec30b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:971803f952d24a18bd9b34477a1ec30b
record_format	dspace
spelling	oai:doaj.org-article:971803f952d24a18bd9b34477a1ec30b2021-11-25T17:08:32ZA Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues10.3390/cells101128852073-4409https://doaj.org/article/971803f952d24a18bd9b34477a1ec30b2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2885https://doaj.org/toc/2073-4409Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells. We recently identified a novel microbiome-derived peptide called corisin that worsens lung fibrosis by inducing apoptosis of lung epithelial cells. We hypothesized that corisin and a corisin-like peptide might also induce apoptosis of cells from different tissues. We cultured podocytes, renal tubular epithelial cells, keratinocytes, retinal and intestinal cells treated with corisin and evaluated apoptosis by flow cytometry and Western blotting. Although at different grades, flow cytometry analysis and Western blotting showed that corisin and a corisin-like peptide induced apoptosis of podocytes, keratinocytes, tubular epithelial cells, retinal, and intestinal cells. In addition, we found that corisin synergistically enhances the proapoptotic activity of transforming growth factor-β1 on podocytes. In conclusion, these results suggest that corisin and corisin-like peptides may play a role in the pathogenesis of disease in different organs by promoting apoptosis of parenchymal cells.Haruko SaikiYuko OkanoTaro YasumaMasaaki TodaAtsuro TakeshitaAhmed M. Abdel-HamidValeria Fridman D’AlessandroTatsuki TsurugaCorina N. D’Alessandro-GabazzaKan KatayamaMasahiko SugimotoHajime FujimotoKeiichi YamanakaTetsu KobayashiIsaac CannEsteban C. GabazzaMDPI AGarticlecorisinapoptosisorgan fibrosisparenchymal cellsdifferent tissueBiology (General)QH301-705.5ENCells, Vol 10, Iss 2885, p 2885 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	corisin apoptosis organ fibrosis parenchymal cells different tissue Biology (General) QH301-705.5
spellingShingle	corisin apoptosis organ fibrosis parenchymal cells different tissue Biology (General) QH301-705.5 Haruko Saiki Yuko Okano Taro Yasuma Masaaki Toda Atsuro Takeshita Ahmed M. Abdel-Hamid Valeria Fridman D’Alessandro Tatsuki Tsuruga Corina N. D’Alessandro-Gabazza Kan Katayama Masahiko Sugimoto Hajime Fujimoto Keiichi Yamanaka Tetsu Kobayashi Isaac Cann Esteban C. Gabazza A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
description	Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells. We recently identified a novel microbiome-derived peptide called corisin that worsens lung fibrosis by inducing apoptosis of lung epithelial cells. We hypothesized that corisin and a corisin-like peptide might also induce apoptosis of cells from different tissues. We cultured podocytes, renal tubular epithelial cells, keratinocytes, retinal and intestinal cells treated with corisin and evaluated apoptosis by flow cytometry and Western blotting. Although at different grades, flow cytometry analysis and Western blotting showed that corisin and a corisin-like peptide induced apoptosis of podocytes, keratinocytes, tubular epithelial cells, retinal, and intestinal cells. In addition, we found that corisin synergistically enhances the proapoptotic activity of transforming growth factor-β1 on podocytes. In conclusion, these results suggest that corisin and corisin-like peptides may play a role in the pathogenesis of disease in different organs by promoting apoptosis of parenchymal cells.
format	article
author	Haruko Saiki Yuko Okano Taro Yasuma Masaaki Toda Atsuro Takeshita Ahmed M. Abdel-Hamid Valeria Fridman D’Alessandro Tatsuki Tsuruga Corina N. D’Alessandro-Gabazza Kan Katayama Masahiko Sugimoto Hajime Fujimoto Keiichi Yamanaka Tetsu Kobayashi Isaac Cann Esteban C. Gabazza
author_facet	Haruko Saiki Yuko Okano Taro Yasuma Masaaki Toda Atsuro Takeshita Ahmed M. Abdel-Hamid Valeria Fridman D’Alessandro Tatsuki Tsuruga Corina N. D’Alessandro-Gabazza Kan Katayama Masahiko Sugimoto Hajime Fujimoto Keiichi Yamanaka Tetsu Kobayashi Isaac Cann Esteban C. Gabazza
author_sort	Haruko Saiki
title	A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_short	A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_full	A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_fullStr	A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_full_unstemmed	A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_sort	microbiome-derived peptide induces apoptosis of cells from different tissues
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/971803f952d24a18bd9b34477a1ec30b
work_keys_str_mv	AT harukosaiki amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT yukookano amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT taroyasuma amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT masaakitoda amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT atsurotakeshita amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT ahmedmabdelhamid amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT valeriafridmandalessandro amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT tatsukitsuruga amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT corinandalessandrogabazza amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT kankatayama amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT masahikosugimoto amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT hajimefujimoto amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT keiichiyamanaka amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT tetsukobayashi amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT isaaccann amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT estebancgabazza amicrobiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT harukosaiki microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT yukookano microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT taroyasuma microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT masaakitoda microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT atsurotakeshita microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT ahmedmabdelhamid microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT valeriafridmandalessandro microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT tatsukitsuruga microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT corinandalessandrogabazza microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT kankatayama microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT masahikosugimoto microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT hajimefujimoto microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT keiichiyamanaka microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT tetsukobayashi microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT isaaccann microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues AT estebancgabazza microbiomederivedpeptideinducesapoptosisofcellsfromdifferenttissues
_version_	1718412666690600960

A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues

Ejemplares similares