Stiffness dependent separation of cells in a microfluidic device.

Abnormal cell mechanical stiffness can point to the development of various diseases including cancers and infections. We report a new microfluidic technique for continuous cell separation utilizing variation in cell stiffness. We use a microfluidic channel decorated by periodic diagonal ridges that...

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Autores principales:	Gonghao Wang, Wenbin Mao, Rebecca Byler, Krishna Patel, Caitlin Henegar, Alexander Alexeev, Todd Sulchek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/971905084f834d49acadba118f8072e3
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spelling	oai:doaj.org-article:971905084f834d49acadba118f8072e32021-11-18T08:50:52ZStiffness dependent separation of cells in a microfluidic device.1932-620310.1371/journal.pone.0075901https://doaj.org/article/971905084f834d49acadba118f8072e32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146787/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Abnormal cell mechanical stiffness can point to the development of various diseases including cancers and infections. We report a new microfluidic technique for continuous cell separation utilizing variation in cell stiffness. We use a microfluidic channel decorated by periodic diagonal ridges that compress the flowing cells in rapid succession. The compression in combination with secondary flows in the ridged microfluidic channel translates each cell perpendicular to the channel axis in proportion to its stiffness. We demonstrate the physical principle of the cell sorting mechanism and show that our microfluidic approach can be effectively used to separate a variety of cell types which are similar in size but of different stiffnesses, spanning a range from 210 Pa to 23 kPa. Atomic force microscopy is used to directly measure the stiffness of the separated cells and we found that the trajectories in the microchannel correlated to stiffness. We have demonstrated that the current processing throughput is 250 cells per second. This microfluidic separation technique opens new ways for conducting rapid and low-cost cell analysis and disease diagnostics through biophysical markers.Gonghao WangWenbin MaoRebecca BylerKrishna PatelCaitlin HenegarAlexander AlexeevTodd SulchekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e75901 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gonghao Wang Wenbin Mao Rebecca Byler Krishna Patel Caitlin Henegar Alexander Alexeev Todd Sulchek Stiffness dependent separation of cells in a microfluidic device.
description	Abnormal cell mechanical stiffness can point to the development of various diseases including cancers and infections. We report a new microfluidic technique for continuous cell separation utilizing variation in cell stiffness. We use a microfluidic channel decorated by periodic diagonal ridges that compress the flowing cells in rapid succession. The compression in combination with secondary flows in the ridged microfluidic channel translates each cell perpendicular to the channel axis in proportion to its stiffness. We demonstrate the physical principle of the cell sorting mechanism and show that our microfluidic approach can be effectively used to separate a variety of cell types which are similar in size but of different stiffnesses, spanning a range from 210 Pa to 23 kPa. Atomic force microscopy is used to directly measure the stiffness of the separated cells and we found that the trajectories in the microchannel correlated to stiffness. We have demonstrated that the current processing throughput is 250 cells per second. This microfluidic separation technique opens new ways for conducting rapid and low-cost cell analysis and disease diagnostics through biophysical markers.
format	article
author	Gonghao Wang Wenbin Mao Rebecca Byler Krishna Patel Caitlin Henegar Alexander Alexeev Todd Sulchek
author_facet	Gonghao Wang Wenbin Mao Rebecca Byler Krishna Patel Caitlin Henegar Alexander Alexeev Todd Sulchek
author_sort	Gonghao Wang
title	Stiffness dependent separation of cells in a microfluidic device.
title_short	Stiffness dependent separation of cells in a microfluidic device.
title_full	Stiffness dependent separation of cells in a microfluidic device.
title_fullStr	Stiffness dependent separation of cells in a microfluidic device.
title_full_unstemmed	Stiffness dependent separation of cells in a microfluidic device.
title_sort	stiffness dependent separation of cells in a microfluidic device.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/971905084f834d49acadba118f8072e3
work_keys_str_mv	AT gonghaowang stiffnessdependentseparationofcellsinamicrofluidicdevice AT wenbinmao stiffnessdependentseparationofcellsinamicrofluidicdevice AT rebeccabyler stiffnessdependentseparationofcellsinamicrofluidicdevice AT krishnapatel stiffnessdependentseparationofcellsinamicrofluidicdevice AT caitlinhenegar stiffnessdependentseparationofcellsinamicrofluidicdevice AT alexanderalexeev stiffnessdependentseparationofcellsinamicrofluidicdevice AT toddsulchek stiffnessdependentseparationofcellsinamicrofluidicdevice
_version_	1718421276342616064

Stiffness dependent separation of cells in a microfluidic device.

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