The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus

Abstract Diatoms are one of the most prominent oceanic primary producers and are now recognized to be distributed throughout the world. They maintain their population despite predators, infections, and unfavourable environmental conditions. One of the smallest diatoms, Chaetoceros tenuissimus, can c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuki Hongo, Kei Kimura, Yoshihiro Takaki, Yukari Yoshida, Shuichiro Baba, Genta Kobayashi, Keizo Nagasaki, Takeshi Hano, Yuji Tomaru
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/971dcd76a553480d9eb551b5a36b7a3d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:971dcd76a553480d9eb551b5a36b7a3d
record_format	dspace
spelling	oai:doaj.org-article:971dcd76a553480d9eb551b5a36b7a3d2021-11-28T12:20:29ZThe genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus10.1038/s41598-021-00565-32045-2322https://doaj.org/article/971dcd76a553480d9eb551b5a36b7a3d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00565-3https://doaj.org/toc/2045-2322Abstract Diatoms are one of the most prominent oceanic primary producers and are now recognized to be distributed throughout the world. They maintain their population despite predators, infections, and unfavourable environmental conditions. One of the smallest diatoms, Chaetoceros tenuissimus, can coexist with infectious viruses during blooms. To further understand this relationship, we sequenced the C. tenuissimus strain NIES-3715 genome. A gene fragment of a replication-associated gene from the infectious ssDNA virus (designated endogenous virus-like fragment, EVLF) was found to be integrated into each 41 Mb of haploid assembly. In addition, the EVLF was transcriptionally active and conserved in nine other C. tenuissimus strains from different geographical areas, although the primary structures of their proteins varied. The phylogenetic tree further suggested that the EVLF was acquired by the ancestor of C. tenuissimus. Additionally, retrotransposon genes possessing a reverse transcriptase function were more abundant in C. tenuissimus than in Thalassiosira pseudonana and Phaeodactylum tricornutum. Moreover, a target site duplication, a hallmark for long interspersed nuclear element retrotransposons, flanked the EVLF. Therefore, the EVLF was likely integrated by a retrotransposon during viral infection. The present study provides further insights into the diatom-virus evolutionary relationship.Yuki HongoKei KimuraYoshihiro TakakiYukari YoshidaShuichiro BabaGenta KobayashiKeizo NagasakiTakeshi HanoYuji TomaruNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Hongo Kei Kimura Yoshihiro Takaki Yukari Yoshida Shuichiro Baba Genta Kobayashi Keizo Nagasaki Takeshi Hano Yuji Tomaru The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
description	Abstract Diatoms are one of the most prominent oceanic primary producers and are now recognized to be distributed throughout the world. They maintain their population despite predators, infections, and unfavourable environmental conditions. One of the smallest diatoms, Chaetoceros tenuissimus, can coexist with infectious viruses during blooms. To further understand this relationship, we sequenced the C. tenuissimus strain NIES-3715 genome. A gene fragment of a replication-associated gene from the infectious ssDNA virus (designated endogenous virus-like fragment, EVLF) was found to be integrated into each 41 Mb of haploid assembly. In addition, the EVLF was transcriptionally active and conserved in nine other C. tenuissimus strains from different geographical areas, although the primary structures of their proteins varied. The phylogenetic tree further suggested that the EVLF was acquired by the ancestor of C. tenuissimus. Additionally, retrotransposon genes possessing a reverse transcriptase function were more abundant in C. tenuissimus than in Thalassiosira pseudonana and Phaeodactylum tricornutum. Moreover, a target site duplication, a hallmark for long interspersed nuclear element retrotransposons, flanked the EVLF. Therefore, the EVLF was likely integrated by a retrotransposon during viral infection. The present study provides further insights into the diatom-virus evolutionary relationship.
format	article
author	Yuki Hongo Kei Kimura Yoshihiro Takaki Yukari Yoshida Shuichiro Baba Genta Kobayashi Keizo Nagasaki Takeshi Hano Yuji Tomaru
author_facet	Yuki Hongo Kei Kimura Yoshihiro Takaki Yukari Yoshida Shuichiro Baba Genta Kobayashi Keizo Nagasaki Takeshi Hano Yuji Tomaru
author_sort	Yuki Hongo
title	The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
title_short	The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
title_full	The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
title_fullStr	The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
title_full_unstemmed	The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
title_sort	genome of the diatom chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/971dcd76a553480d9eb551b5a36b7a3d
work_keys_str_mv	AT yukihongo thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT keikimura thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yoshihirotakaki thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yukariyoshida thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT shuichirobaba thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT gentakobayashi thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT keizonagasaki thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT takeshihano thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yujitomaru thegenomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yukihongo genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT keikimura genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yoshihirotakaki genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yukariyoshida genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT shuichirobaba genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT gentakobayashi genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT keizonagasaki genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT takeshihano genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus AT yujitomaru genomeofthediatomchaetocerostenuissimuscarriesanancientintegratedfragmentofanextantvirus
_version_	1718408030976999424

The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus

Ejemplares similares