Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay

Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the freque...

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Autores principales:	Giuseppa Purpari, Francesco Mira, Santina Di Bella, Simona Di Pietro, Elisabetta Giudice, Annalisa Guercio
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	polymerase chain reaction canine parvovirus virus isolation Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/971f95bb9b974c2784d782762afb0213
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spelling	oai:doaj.org-article:971f95bb9b974c2784d782762afb02132021-11-17T21:27:52ZInvestigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay1820-744810.2478/acve-2018-0007https://doaj.org/article/971f95bb9b974c2784d782762afb02132018-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0007https://doaj.org/toc/1820-7448Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and these were further analyzed by restriction fragment length polymorphism (RFLP) and DNA sequence analysis. A high prevalence of the CPV-2c variant (79.56% of CPV-2 positive dogs) was observed in this survey, underlining that this type of CPV field isolate is prevalent circulating in Sicily. Sequence and phylogenetic analysis showed a close relationship with CPV-2a and CPV-2c strains from Europe and non-European countries. Considering that CPV-2c is reaching a worldwide distribution and that this variant is also affecting vaccinated dogs, efforts should be made towards the development of new CPV vaccines.Giuseppa PurpariFrancesco MiraSantina Di BellaSimona Di PietroElisabetta GiudiceAnnalisa GuercioSciendoarticlepolymerase chain reactioncanine parvovirusvirus isolationVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 1, Pp 80-94 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	polymerase chain reaction canine parvovirus virus isolation Veterinary medicine SF600-1100
spellingShingle	polymerase chain reaction canine parvovirus virus isolation Veterinary medicine SF600-1100 Giuseppa Purpari Francesco Mira Santina Di Bella Simona Di Pietro Elisabetta Giudice Annalisa Guercio Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
description	Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and these were further analyzed by restriction fragment length polymorphism (RFLP) and DNA sequence analysis. A high prevalence of the CPV-2c variant (79.56% of CPV-2 positive dogs) was observed in this survey, underlining that this type of CPV field isolate is prevalent circulating in Sicily. Sequence and phylogenetic analysis showed a close relationship with CPV-2a and CPV-2c strains from Europe and non-European countries. Considering that CPV-2c is reaching a worldwide distribution and that this variant is also affecting vaccinated dogs, efforts should be made towards the development of new CPV vaccines.
format	article
author	Giuseppa Purpari Francesco Mira Santina Di Bella Simona Di Pietro Elisabetta Giudice Annalisa Guercio
author_facet	Giuseppa Purpari Francesco Mira Santina Di Bella Simona Di Pietro Elisabetta Giudice Annalisa Guercio
author_sort	Giuseppa Purpari
title	Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
title_short	Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
title_full	Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
title_fullStr	Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
title_full_unstemmed	Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
title_sort	investigation on canine parvovirus circulation in dogs from sicily (italy) by biomolecular assay
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/971f95bb9b974c2784d782762afb0213
work_keys_str_mv	AT giuseppapurpari investigationoncanineparvoviruscirculationindogsfromsicilyitalybybiomolecularassay AT francescomira investigationoncanineparvoviruscirculationindogsfromsicilyitalybybiomolecularassay AT santinadibella investigationoncanineparvoviruscirculationindogsfromsicilyitalybybiomolecularassay AT simonadipietro investigationoncanineparvoviruscirculationindogsfromsicilyitalybybiomolecularassay AT elisabettagiudice investigationoncanineparvoviruscirculationindogsfromsicilyitalybybiomolecularassay AT annalisaguercio investigationoncanineparvoviruscirculationindogsfromsicilyitalybybiomolecularassay
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Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay

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