Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway

Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S (BPS) and bisphenol F (BPF), which hav...

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Autores principales:	Hongjie Fan, Sudini R. Fernando, Luhan Jiang, Ziyi Wang, Suranga P. Kodithuwakku, Chris K. C. Wong, Ernest H. Y. Ng, William S. B. Yeung, Kai-Fai Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bisphenols co-culture spheroid attachment steroid hormones endometrium microarray Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/971feabb6ea342529e1cf94674d16353
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spelling	oai:doaj.org-article:971feabb6ea342529e1cf94674d163532021-11-25T17:08:31ZBisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway10.3390/cells101128822073-4409https://doaj.org/article/971feabb6ea342529e1cf94674d163532021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2882https://doaj.org/toc/2073-4409Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S (BPS) and bisphenol F (BPF), which have a similar chemical structure to BPA. Compared to BPA, both BPS and BPF have weaker estrogenic effects, but their effects on human reproductive function including endometrial receptivity and embryo implantation still remain largely unknown. We used an in vitro spheroid (blastocyst surrogate) co-culture assay to investigate the effects of BPA, BPS, and BPF on spheroid attachment on human endometrial epithelial cells, and further delineated their role on steroid hormone receptor expression. We also used transcriptomics to investigate the effects of BPA, BPS, and BPF on the transcriptome of human endometrial cells. We found that bisphenol treatment in human endometrial Ishikawa cells altered estrogen receptor alpha (ERα) signaling and upregulated progesterone receptors (PR). Bisphenols suppressed spheroid attachment onto Ishikawa cells, which was reversed by the downregulation of PR through PR siRNA. Overall, we found that bisphenol compounds can affect human endometrial epithelial cell receptivity through the modulation of steroid hormone receptor function leading to impaired embryo implantation.Hongjie FanSudini R. FernandoLuhan JiangZiyi WangSuranga P. KodithuwakkuChris K. C. WongErnest H. Y. NgWilliam S. B. YeungKai-Fai LeeMDPI AGarticlebisphenolsco-culturespheroid attachmentsteroid hormonesendometriummicroarrayBiology (General)QH301-705.5ENCells, Vol 10, Iss 2882, p 2882 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bisphenols co-culture spheroid attachment steroid hormones endometrium microarray Biology (General) QH301-705.5
spellingShingle	bisphenols co-culture spheroid attachment steroid hormones endometrium microarray Biology (General) QH301-705.5 Hongjie Fan Sudini R. Fernando Luhan Jiang Ziyi Wang Suranga P. Kodithuwakku Chris K. C. Wong Ernest H. Y. Ng William S. B. Yeung Kai-Fai Lee Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
description	Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S (BPS) and bisphenol F (BPF), which have a similar chemical structure to BPA. Compared to BPA, both BPS and BPF have weaker estrogenic effects, but their effects on human reproductive function including endometrial receptivity and embryo implantation still remain largely unknown. We used an in vitro spheroid (blastocyst surrogate) co-culture assay to investigate the effects of BPA, BPS, and BPF on spheroid attachment on human endometrial epithelial cells, and further delineated their role on steroid hormone receptor expression. We also used transcriptomics to investigate the effects of BPA, BPS, and BPF on the transcriptome of human endometrial cells. We found that bisphenol treatment in human endometrial Ishikawa cells altered estrogen receptor alpha (ERα) signaling and upregulated progesterone receptors (PR). Bisphenols suppressed spheroid attachment onto Ishikawa cells, which was reversed by the downregulation of PR through PR siRNA. Overall, we found that bisphenol compounds can affect human endometrial epithelial cell receptivity through the modulation of steroid hormone receptor function leading to impaired embryo implantation.
format	article
author	Hongjie Fan Sudini R. Fernando Luhan Jiang Ziyi Wang Suranga P. Kodithuwakku Chris K. C. Wong Ernest H. Y. Ng William S. B. Yeung Kai-Fai Lee
author_facet	Hongjie Fan Sudini R. Fernando Luhan Jiang Ziyi Wang Suranga P. Kodithuwakku Chris K. C. Wong Ernest H. Y. Ng William S. B. Yeung Kai-Fai Lee
author_sort	Hongjie Fan
title	Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
title_short	Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
title_full	Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
title_fullStr	Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
title_full_unstemmed	Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
title_sort	bisphenol a analogues suppress spheroid attachment on human endometrial epithelial cells through modulation of steroid hormone receptors signaling pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/971feabb6ea342529e1cf94674d16353
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Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway

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