Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells

Oleanolic acid (OA), asiatic acid (AA), and maslinic acid (MA) are ubiquitous isomeric triterpene phytochemicals with many pharmacological effects. To improve their application value, we used lipopolysaccharide (LPS) to induce RAW264.7 cells and studied the differences in the anti-inflammatory effec...

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Autores principales:	Yu Han, Chen Yuan, Xiaowei Zhou, Yingjie Han, Yanhao He, Jian Ouyang, Wenna Zhou, Zhenhua Wang, Honglun Wang, Gang Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	inflammation RAW264.7 cell oleanolic acid asiatic acid maslinic acid NF-κB Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9727ce637fb341d288eb11140bb311e4
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spelling	oai:doaj.org-article:9727ce637fb341d288eb11140bb311e42021-11-11T17:25:38ZAnti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells10.3390/ijms2221120091422-00671661-6596https://doaj.org/article/9727ce637fb341d288eb11140bb311e42021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12009https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Oleanolic acid (OA), asiatic acid (AA), and maslinic acid (MA) are ubiquitous isomeric triterpene phytochemicals with many pharmacological effects. To improve their application value, we used lipopolysaccharide (LPS) to induce RAW264.7 cells and studied the differences in the anti-inflammatory effects of the triterpenes according to their structural differences. MTT, Griess, and immunofluorescence assays, ELISA, flow cytometry, and Western blotting, were performed. The release of LPS-induced pro-inflammatory mediators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), and interleukin (IL-6), was significantly inhibited by OA, AA, and MA at the same concentration, and AA and MA promoted the production of anti-inflammatory factor IL-10. OA, AA, and MA inhibited LPS-induced NF-κB nuclear translocation in RAW264.7 cells. OA and AA inhibited the phosphorylation of ERK1/2, P38, and JNK1/2 in LPS-stimulated RAW264.7 cells. Moreover, OA increased LPS-induced Nrf2 expression and decreased Keap1 expression in RAW264.7 cells. OA, AA, and MA inhibited LPS-stimulated intracellular reactive oxygen species (ROS) production and alleviated mitochondrial membrane potential depletion. Overall, our data suggested that OA, AA, and MA exhibited significant anti-inflammatory effects in vitro. In particular, OA and AA take effects through the MAPKs, NF-κB, and Nrf2 signaling pathways.Yu HanChen YuanXiaowei ZhouYingjie HanYanhao HeJian OuyangWenna ZhouZhenhua WangHonglun WangGang LiMDPI AGarticleinflammationRAW264.7 celloleanolic acidasiatic acidmaslinic acidNF-κBBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12009, p 12009 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammation RAW264.7 cell oleanolic acid asiatic acid maslinic acid NF-κB Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	inflammation RAW264.7 cell oleanolic acid asiatic acid maslinic acid NF-κB Biology (General) QH301-705.5 Chemistry QD1-999 Yu Han Chen Yuan Xiaowei Zhou Yingjie Han Yanhao He Jian Ouyang Wenna Zhou Zhenhua Wang Honglun Wang Gang Li Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
description	Oleanolic acid (OA), asiatic acid (AA), and maslinic acid (MA) are ubiquitous isomeric triterpene phytochemicals with many pharmacological effects. To improve their application value, we used lipopolysaccharide (LPS) to induce RAW264.7 cells and studied the differences in the anti-inflammatory effects of the triterpenes according to their structural differences. MTT, Griess, and immunofluorescence assays, ELISA, flow cytometry, and Western blotting, were performed. The release of LPS-induced pro-inflammatory mediators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), and interleukin (IL-6), was significantly inhibited by OA, AA, and MA at the same concentration, and AA and MA promoted the production of anti-inflammatory factor IL-10. OA, AA, and MA inhibited LPS-induced NF-κB nuclear translocation in RAW264.7 cells. OA and AA inhibited the phosphorylation of ERK1/2, P38, and JNK1/2 in LPS-stimulated RAW264.7 cells. Moreover, OA increased LPS-induced Nrf2 expression and decreased Keap1 expression in RAW264.7 cells. OA, AA, and MA inhibited LPS-stimulated intracellular reactive oxygen species (ROS) production and alleviated mitochondrial membrane potential depletion. Overall, our data suggested that OA, AA, and MA exhibited significant anti-inflammatory effects in vitro. In particular, OA and AA take effects through the MAPKs, NF-κB, and Nrf2 signaling pathways.
format	article
author	Yu Han Chen Yuan Xiaowei Zhou Yingjie Han Yanhao He Jian Ouyang Wenna Zhou Zhenhua Wang Honglun Wang Gang Li
author_facet	Yu Han Chen Yuan Xiaowei Zhou Yingjie Han Yanhao He Jian Ouyang Wenna Zhou Zhenhua Wang Honglun Wang Gang Li
author_sort	Yu Han
title	Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
title_short	Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
title_full	Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
title_fullStr	Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
title_full_unstemmed	Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
title_sort	anti-inflammatory activity of three triterpene from <i>hippophae rhamnoides</i> l. in lipopolysaccharide-stimulated raw264.7 cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9727ce637fb341d288eb11140bb311e4
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Anti-Inflammatory Activity of Three Triterpene from <i>Hippophae rhamnoides</i> L. in Lipopolysaccharide-Stimulated RAW264.7 Cells

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