Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust

Abstract Locusts are advantageous organisms to elucidate mechanisms of olfactory coding at the systems level. Sensory input is provided by the olfactory receptor neurons of the antenna, which send their axons into the antennal lobe. So far, cellular properties of neurons isolated from the circuitry...

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Autores principales:	Gregor A. Bergmann, Gerd Bicker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/972b9602243642bc852845b8c55fea8c
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spelling	oai:doaj.org-article:972b9602243642bc852845b8c55fea8c2021-12-02T14:35:34ZCholinergic calcium responses in cultured antennal lobe neurons of the migratory locust10.1038/s41598-021-89374-22045-2322https://doaj.org/article/972b9602243642bc852845b8c55fea8c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89374-2https://doaj.org/toc/2045-2322Abstract Locusts are advantageous organisms to elucidate mechanisms of olfactory coding at the systems level. Sensory input is provided by the olfactory receptor neurons of the antenna, which send their axons into the antennal lobe. So far, cellular properties of neurons isolated from the circuitry of the olfactory system, such as transmitter-induced calcium responses, have not been studied. Biochemical and immunocytochemical investigations have provided evidence for acetylcholine as classical transmitter of olfactory receptor neurons. Here, we characterize cell cultured projection and local interneurons of the antennal lobe by cytosolic calcium imaging to cholinergic stimulation. We bulk loaded the indicator dye Cal-520 AM in dissociated culture and recorded calcium transients after applying cholinergic agonists and antagonists. The majority of projection and local neurons respond with increases in calcium levels to activation of both nicotinic and muscarinic receptors. In local interneurons, we reveal interactions lasting over minutes between intracellular signaling pathways, mediated by muscarinic and nicotinic receptor stimulation. The present investigation is pioneer in showing that Cal-520 AM readily loads Locusta migratoria neurons, making it a valuable tool for future research in locust neurophysiology, neuropharmacology, and neurodevelopment.Gregor A. BergmannGerd BickerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gregor A. Bergmann Gerd Bicker Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
description	Abstract Locusts are advantageous organisms to elucidate mechanisms of olfactory coding at the systems level. Sensory input is provided by the olfactory receptor neurons of the antenna, which send their axons into the antennal lobe. So far, cellular properties of neurons isolated from the circuitry of the olfactory system, such as transmitter-induced calcium responses, have not been studied. Biochemical and immunocytochemical investigations have provided evidence for acetylcholine as classical transmitter of olfactory receptor neurons. Here, we characterize cell cultured projection and local interneurons of the antennal lobe by cytosolic calcium imaging to cholinergic stimulation. We bulk loaded the indicator dye Cal-520 AM in dissociated culture and recorded calcium transients after applying cholinergic agonists and antagonists. The majority of projection and local neurons respond with increases in calcium levels to activation of both nicotinic and muscarinic receptors. In local interneurons, we reveal interactions lasting over minutes between intracellular signaling pathways, mediated by muscarinic and nicotinic receptor stimulation. The present investigation is pioneer in showing that Cal-520 AM readily loads Locusta migratoria neurons, making it a valuable tool for future research in locust neurophysiology, neuropharmacology, and neurodevelopment.
format	article
author	Gregor A. Bergmann Gerd Bicker
author_facet	Gregor A. Bergmann Gerd Bicker
author_sort	Gregor A. Bergmann
title	Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
title_short	Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
title_full	Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
title_fullStr	Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
title_full_unstemmed	Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
title_sort	cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/972b9602243642bc852845b8c55fea8c
work_keys_str_mv	AT gregorabergmann cholinergiccalciumresponsesinculturedantennallobeneuronsofthemigratorylocust AT gerdbicker cholinergiccalciumresponsesinculturedantennallobeneuronsofthemigratorylocust
_version_	1718391086621130752

Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust

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