SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals

Abstract The aim of this study is to understand adaptive immunity to SARS-CoV-2 through the analysis of B cell epitope and neutralizing activity in coronavirus disease 2019 (COVID-19) patients. We obtained serum from forty-three COVID-19 patients from patients in the intensive care unit of Osaka Uni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shota Yoshida, Chikako Ono, Hiroki Hayashi, Shinya Fukumoto, Satoshi Shiraishi, Kazunori Tomono, Hisashi Arase, Yoshiharu Matsuura, Hironori Nakagami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/972cb6e1631a4f828f36760167864d12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:972cb6e1631a4f828f36760167864d12
record_format	dspace
spelling	oai:doaj.org-article:972cb6e1631a4f828f36760167864d122021-12-02T13:17:49ZSARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals10.1038/s41598-021-85202-92045-2322https://doaj.org/article/972cb6e1631a4f828f36760167864d122021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85202-9https://doaj.org/toc/2045-2322Abstract The aim of this study is to understand adaptive immunity to SARS-CoV-2 through the analysis of B cell epitope and neutralizing activity in coronavirus disease 2019 (COVID-19) patients. We obtained serum from forty-three COVID-19 patients from patients in the intensive care unit of Osaka University Hospital (n = 12) and in Osaka City Juso Hospital (n = 31). Most individuals revealed neutralizing activity against SARS-CoV-2 assessed by a pseudotype virus-neutralizing assay. The antibody production against the spike glycoprotein (S protein) or receptor-binding domain (RBD) of SARS-CoV-2 was elevated, with large individual differences, as assessed by ELISA. We observed the correlation between neutralizing antibody titer and IgG, but not IgM, antibody titer of COVID-19 patients. In the analysis of the predicted the linear B cell epitopes, hot spots in the N-terminal domain of the S protein were observed in the serum from patients in the intensive care unit of Osaka University Hospital. Overall, the analysis of antibody production and B cell epitopes of the S protein from patient serum may provide a novel target for the vaccine development against SARS-CoV-2.Shota YoshidaChikako OnoHiroki HayashiShinya FukumotoSatoshi ShiraishiKazunori TomonoHisashi AraseYoshiharu MatsuuraHironori NakagamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shota Yoshida Chikako Ono Hiroki Hayashi Shinya Fukumoto Satoshi Shiraishi Kazunori Tomono Hisashi Arase Yoshiharu Matsuura Hironori Nakagami SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals
description	Abstract The aim of this study is to understand adaptive immunity to SARS-CoV-2 through the analysis of B cell epitope and neutralizing activity in coronavirus disease 2019 (COVID-19) patients. We obtained serum from forty-three COVID-19 patients from patients in the intensive care unit of Osaka University Hospital (n = 12) and in Osaka City Juso Hospital (n = 31). Most individuals revealed neutralizing activity against SARS-CoV-2 assessed by a pseudotype virus-neutralizing assay. The antibody production against the spike glycoprotein (S protein) or receptor-binding domain (RBD) of SARS-CoV-2 was elevated, with large individual differences, as assessed by ELISA. We observed the correlation between neutralizing antibody titer and IgG, but not IgM, antibody titer of COVID-19 patients. In the analysis of the predicted the linear B cell epitopes, hot spots in the N-terminal domain of the S protein were observed in the serum from patients in the intensive care unit of Osaka University Hospital. Overall, the analysis of antibody production and B cell epitopes of the S protein from patient serum may provide a novel target for the vaccine development against SARS-CoV-2.
format	article
author	Shota Yoshida Chikako Ono Hiroki Hayashi Shinya Fukumoto Satoshi Shiraishi Kazunori Tomono Hisashi Arase Yoshiharu Matsuura Hironori Nakagami
author_facet	Shota Yoshida Chikako Ono Hiroki Hayashi Shinya Fukumoto Satoshi Shiraishi Kazunori Tomono Hisashi Arase Yoshiharu Matsuura Hironori Nakagami
author_sort	Shota Yoshida
title	SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals
title_short	SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals
title_full	SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals
title_fullStr	SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals
title_full_unstemmed	SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals
title_sort	sars-cov-2-induced humoral immunity through b cell epitope analysis in covid-19 infected individuals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/972cb6e1631a4f828f36760167864d12
work_keys_str_mv	AT shotayoshida sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT chikakoono sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT hirokihayashi sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT shinyafukumoto sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT satoshishiraishi sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT kazunoritomono sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT hisashiarase sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT yoshiharumatsuura sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals AT hironorinakagami sarscov2inducedhumoralimmunitythroughbcellepitopeanalysisincovid19infectedindividuals
_version_	1718393345345060864

SARS-CoV-2-induced humoral immunity through B cell epitope analysis in COVID-19 infected individuals

Ejemplares similares