Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area.
<h4>Background</h4>The dengue vaccine (Dengvaxia) is only recommended for individuals with prior dengue infection (PDI). This study aimed to perform a serosurvey to inform decision-making for vaccine introduction and identify appropriate target populations. We also evaluated the performa...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9738708397ce4e9797391463142bc414 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9738708397ce4e9797391463142bc414 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9738708397ce4e9797391463142bc4142021-12-02T20:08:18ZDengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area.1932-620310.1371/journal.pone.0257182https://doaj.org/article/9738708397ce4e9797391463142bc4142021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257182https://doaj.org/toc/1932-6203<h4>Background</h4>The dengue vaccine (Dengvaxia) is only recommended for individuals with prior dengue infection (PDI). This study aimed to perform a serosurvey to inform decision-making for vaccine introduction and identify appropriate target populations. We also evaluated the performance of the serological tests using plaque reduction neutralization test (PRNT) as a reference test in identifying PDI to determine suitability for pre-vaccination screening.<h4>Methods</h4>We enrolled 115 healthy individuals between 10 and 22 years of age living in the Ratchaburi province of Thailand. The serum samples were tested by PRNT to measure the prevalence and concentration of serotype-specific neutralizing antibodies. The performance of the IgG rapid diagnostic test (RDT, SD Bioline, Korea) and IgG enzyme-linked immunosorbent assay (ELISA, EUROIMMUN, Germany) in identifying PDI were evaluated by using PRNT as a reference method.<h4>Results</h4>Ninety-four (81.7%) individuals neutralized one or more dengue serotypes at a titer threshold greater than or equal to 10. Multitypic profiles were observed in 70.4% of the samples which increased to 91.9% in subjects aged 19-22. Among monotypic samples, the highest proportion was reactive against DENV-1 followed by DENV-2, DENV-3, and DENV-4. The highest anti-dengue antibody titers were recorded against DENV-1 and increased with age to a geometric mean NT50 titer (GMT) of 188.6 in the 19-22 age group. While both RDT and ELISA exhibited 100% specificity, RDT demonstrated low sensitivity (35%) with ELISA displaying much greater sensitivity (87%).<h4>Conclusions</h4>Almost 80% of adolescents and youth in Ratchaburi province had already been exposed to one or more of the dengue virus serotypes. The dengue IgG RDT displayed low sensitivity and is likely not be suitable for dengue pre-vaccination screening. These results support the use of IgG ELISA test for dengue vaccination in endemic areas.Umaporn LimothaiSasipha TachaboonJanejira DinhuzenTaweewun HunsawongPrapapun Ong-AjchaowlerdButsaya ThaisomboonsukStefan FernandezSupachoke TrongkamolchaiMananya WanpaisitkulChatchai ChulapornsiriAnongrat TiawilaiThawat TiawilaiTerapong TantawichienUsa ThisyakornNattachai SrisawatPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257182 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Umaporn Limothai Sasipha Tachaboon Janejira Dinhuzen Taweewun Hunsawong Prapapun Ong-Ajchaowlerd Butsaya Thaisomboonsuk Stefan Fernandez Supachoke Trongkamolchai Mananya Wanpaisitkul Chatchai Chulapornsiri Anongrat Tiawilai Thawat Tiawilai Terapong Tantawichien Usa Thisyakorn Nattachai Srisawat Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| description |
<h4>Background</h4>The dengue vaccine (Dengvaxia) is only recommended for individuals with prior dengue infection (PDI). This study aimed to perform a serosurvey to inform decision-making for vaccine introduction and identify appropriate target populations. We also evaluated the performance of the serological tests using plaque reduction neutralization test (PRNT) as a reference test in identifying PDI to determine suitability for pre-vaccination screening.<h4>Methods</h4>We enrolled 115 healthy individuals between 10 and 22 years of age living in the Ratchaburi province of Thailand. The serum samples were tested by PRNT to measure the prevalence and concentration of serotype-specific neutralizing antibodies. The performance of the IgG rapid diagnostic test (RDT, SD Bioline, Korea) and IgG enzyme-linked immunosorbent assay (ELISA, EUROIMMUN, Germany) in identifying PDI were evaluated by using PRNT as a reference method.<h4>Results</h4>Ninety-four (81.7%) individuals neutralized one or more dengue serotypes at a titer threshold greater than or equal to 10. Multitypic profiles were observed in 70.4% of the samples which increased to 91.9% in subjects aged 19-22. Among monotypic samples, the highest proportion was reactive against DENV-1 followed by DENV-2, DENV-3, and DENV-4. The highest anti-dengue antibody titers were recorded against DENV-1 and increased with age to a geometric mean NT50 titer (GMT) of 188.6 in the 19-22 age group. While both RDT and ELISA exhibited 100% specificity, RDT demonstrated low sensitivity (35%) with ELISA displaying much greater sensitivity (87%).<h4>Conclusions</h4>Almost 80% of adolescents and youth in Ratchaburi province had already been exposed to one or more of the dengue virus serotypes. The dengue IgG RDT displayed low sensitivity and is likely not be suitable for dengue pre-vaccination screening. These results support the use of IgG ELISA test for dengue vaccination in endemic areas. |
| format |
article |
| author |
Umaporn Limothai Sasipha Tachaboon Janejira Dinhuzen Taweewun Hunsawong Prapapun Ong-Ajchaowlerd Butsaya Thaisomboonsuk Stefan Fernandez Supachoke Trongkamolchai Mananya Wanpaisitkul Chatchai Chulapornsiri Anongrat Tiawilai Thawat Tiawilai Terapong Tantawichien Usa Thisyakorn Nattachai Srisawat |
| author_facet |
Umaporn Limothai Sasipha Tachaboon Janejira Dinhuzen Taweewun Hunsawong Prapapun Ong-Ajchaowlerd Butsaya Thaisomboonsuk Stefan Fernandez Supachoke Trongkamolchai Mananya Wanpaisitkul Chatchai Chulapornsiri Anongrat Tiawilai Thawat Tiawilai Terapong Tantawichien Usa Thisyakorn Nattachai Srisawat |
| author_sort |
Umaporn Limothai |
| title |
Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| title_short |
Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| title_full |
Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| title_fullStr |
Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| title_full_unstemmed |
Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| title_sort |
dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/9738708397ce4e9797391463142bc414 |
| work_keys_str_mv |
AT umapornlimothai dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT sasiphatachaboon dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT janejiradinhuzen dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT taweewunhunsawong dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT prapapunongajchaowlerd dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT butsayathaisomboonsuk dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT stefanfernandez dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT supachoketrongkamolchai dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT mananyawanpaisitkul dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT chatchaichulapornsiri dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT anongrattiawilai dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT thawattiawilai dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT terapongtantawichien dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT usathisyakorn dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea AT nattachaisrisawat dengueprevaccinationscreeningtestevaluationfortheuseofdenguevaccineinanendemicarea |
| _version_ |
1718375196659810304 |