FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION

Assessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. This paper is about how to implement resazurin reduction method as an indirect indicator of cell...

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Autores principales:	Linamaría Escobar M., Fabio A. Aristizábal G.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2010
Materias:	in vitro assay citotoxicity cell lines resazurin Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/973aa2749371435d943cd383cd09db47
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spelling	oai:doaj.org-article:973aa2749371435d943cd383cd09db472021-11-19T04:14:24ZFLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION0121-40042145-2660https://doaj.org/article/973aa2749371435d943cd383cd09db472010-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/6342https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Assessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. This paper is about how to implement resazurin reduction method as an indirect indicator of cell survival. Indeed both optimal resazurin concentration and incubation time were selected on MCF-7, MKN-45, SiHa, HEp-2, HT-29 and HeLa. Next, we compared survival results by using reduction method MTT and staining method SRB defined as end-point tests, and resazurin method described as slightly toxic to cells. The comparison of the methods was done in terms of survival rate to evaluate the sensitivity of the lines to doxorubicin HCl, a medicine used in cancer. The methods were compared in terms of percentage survival by evaluating cell line sensitivity to doxorubicin HCl (a drug used in cancer treatment). The results show that resazurin reduction method used in 4.4 μM is applicable to detect significant changes in cell population exposed to cytotoxic molecules in routine testing.Linamaría Escobar M.Fabio A. Aristizábal G.Universidad de Antioquiaarticlein vitro assaycitotoxicitycell linesresazurinFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 17, Iss 2 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vitro assay citotoxicity cell lines resazurin Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	in vitro assay citotoxicity cell lines resazurin Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Linamaría Escobar M. Fabio A. Aristizábal G. FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
description	Assessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. This paper is about how to implement resazurin reduction method as an indirect indicator of cell survival. Indeed both optimal resazurin concentration and incubation time were selected on MCF-7, MKN-45, SiHa, HEp-2, HT-29 and HeLa. Next, we compared survival results by using reduction method MTT and staining method SRB defined as end-point tests, and resazurin method described as slightly toxic to cells. The comparison of the methods was done in terms of survival rate to evaluate the sensitivity of the lines to doxorubicin HCl, a medicine used in cancer. The methods were compared in terms of percentage survival by evaluating cell line sensitivity to doxorubicin HCl (a drug used in cancer treatment). The results show that resazurin reduction method used in 4.4 μM is applicable to detect significant changes in cell population exposed to cytotoxic molecules in routine testing.
format	article
author	Linamaría Escobar M. Fabio A. Aristizábal G.
author_facet	Linamaría Escobar M. Fabio A. Aristizábal G.
author_sort	Linamaría Escobar M.
title	FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
title_short	FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
title_full	FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
title_fullStr	FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
title_full_unstemmed	FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
title_sort	fluorometric assay for cell proliferation in human tumor cell lines application
publisher	Universidad de Antioquia
publishDate	2010
url	https://doaj.org/article/973aa2749371435d943cd383cd09db47
work_keys_str_mv	AT linamariaescobarm fluorometricassayforcellproliferationinhumantumorcelllinesapplication AT fabioaaristizabalg fluorometricassayforcellproliferationinhumantumorcelllinesapplication
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FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION

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