Short Communication: Identification of Leptin gene in crossbred beef cattle

Abstract. Anugratama LE, Hartatik T. 2020. Short Communication: Identification of Leptin gene in crossbred beef cattle. Biodiversitas 21: 226-230. Leptin is a gene that affects animal weight. Leptin gene is known to control body weight, feed intake, energy expenditure, immune function, and reproduct...

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Autores principales:	Laksa Ersa Anugratama, Tety Hartatik
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	genbank, leptin gene, phylogenetic, single nucleotide polymorphism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/973c95233e9e461fbe754c8e18f4f87c
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spelling	oai:doaj.org-article:973c95233e9e461fbe754c8e18f4f87c2021-11-21T22:09:24ZShort Communication: Identification of Leptin gene in crossbred beef cattle1412-033X2085-472210.13057/biodiv/d210129https://doaj.org/article/973c95233e9e461fbe754c8e18f4f87c2019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4812https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Anugratama LE, Hartatik T. 2020. Short Communication: Identification of Leptin gene in crossbred beef cattle. Biodiversitas 21: 226-230. Leptin is a gene that affects animal weight. Leptin gene is known to control body weight, feed intake, energy expenditure, immune function, and reproduction. This study aims to identify the diversity of the Leptin gene in crossbred beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, buffalo, sheep, and goat by comparing with four GenBank data of cattle. Crossbred beef cattle obtained from Klaten, Central Java, Indonesia. Leptin nucleotide sequences were analyzed using BioEdit to identify Single Nucleotide Polymorphism (SNP). To create amino acid change in Leptin gene, the coding sequence of exon 2 was established using BioEdit ver. 7.0.5. Phylogenetic tree and genetic distance have been analyzed based on the Leptin gene using MEGA 10.1.1 program. The result shows that eight variations of SNP were found in exon 2. The phylogenetic tree represents that crossbreed beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, Bos taurus, Bos indicus, Bos frontalis, Bos grunniens, Bubalus bubalis are in the same cluster with various genetic distance. The results of this study are expected to provide genetic information that will be used for further research on the relationship between Leptin gene polymorphisms to animal weight.Laksa Ersa AnugratamaTety HartatikMBI & UNS Soloarticlegenbank, leptin gene, phylogenetic, single nucleotide polymorphismBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	genbank, leptin gene, phylogenetic, single nucleotide polymorphism Biology (General) QH301-705.5
spellingShingle	genbank, leptin gene, phylogenetic, single nucleotide polymorphism Biology (General) QH301-705.5 Laksa Ersa Anugratama Tety Hartatik Short Communication: Identification of Leptin gene in crossbred beef cattle
description	Abstract. Anugratama LE, Hartatik T. 2020. Short Communication: Identification of Leptin gene in crossbred beef cattle. Biodiversitas 21: 226-230. Leptin is a gene that affects animal weight. Leptin gene is known to control body weight, feed intake, energy expenditure, immune function, and reproduction. This study aims to identify the diversity of the Leptin gene in crossbred beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, buffalo, sheep, and goat by comparing with four GenBank data of cattle. Crossbred beef cattle obtained from Klaten, Central Java, Indonesia. Leptin nucleotide sequences were analyzed using BioEdit to identify Single Nucleotide Polymorphism (SNP). To create amino acid change in Leptin gene, the coding sequence of exon 2 was established using BioEdit ver. 7.0.5. Phylogenetic tree and genetic distance have been analyzed based on the Leptin gene using MEGA 10.1.1 program. The result shows that eight variations of SNP were found in exon 2. The phylogenetic tree represents that crossbreed beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, Bos taurus, Bos indicus, Bos frontalis, Bos grunniens, Bubalus bubalis are in the same cluster with various genetic distance. The results of this study are expected to provide genetic information that will be used for further research on the relationship between Leptin gene polymorphisms to animal weight.
format	article
author	Laksa Ersa Anugratama Tety Hartatik
author_facet	Laksa Ersa Anugratama Tety Hartatik
author_sort	Laksa Ersa Anugratama
title	Short Communication: Identification of Leptin gene in crossbred beef cattle
title_short	Short Communication: Identification of Leptin gene in crossbred beef cattle
title_full	Short Communication: Identification of Leptin gene in crossbred beef cattle
title_fullStr	Short Communication: Identification of Leptin gene in crossbred beef cattle
title_full_unstemmed	Short Communication: Identification of Leptin gene in crossbred beef cattle
title_sort	short communication: identification of leptin gene in crossbred beef cattle
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/973c95233e9e461fbe754c8e18f4f87c
work_keys_str_mv	AT laksaersaanugratama shortcommunicationidentificationofleptingeneincrossbredbeefcattle AT tetyhartatik shortcommunicationidentificationofleptingeneincrossbredbeefcattle
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Short Communication: Identification of Leptin gene in crossbred beef cattle

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