Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood

Abstract The increase of the level of glucose in blood leads to an increase in the fraction of glycated hemoglobin (HbA1c). Therefore, the percentage of HbA1c in the blood can serve as a marker for the average glucose level over the past three months and thus, it can be used to diagnose diabetes. He...

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Autores principales:	Shimaa Eissa, Mohammed Zourob
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/973ca5ce414745bbabf7c39c100de13d
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spelling	oai:doaj.org-article:973ca5ce414745bbabf7c39c100de13d2021-12-02T11:53:08ZAptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood10.1038/s41598-017-01226-02045-2322https://doaj.org/article/973ca5ce414745bbabf7c39c100de13d2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01226-0https://doaj.org/toc/2045-2322Abstract The increase of the level of glucose in blood leads to an increase in the fraction of glycated hemoglobin (HbA1c). Therefore, the percentage of HbA1c in the blood can serve as a marker for the average glucose level over the past three months and thus, it can be used to diagnose diabetes. Here, we report the selection, identification and characterization of specific DNA aptamers against HbA1c- and total hemoglobin (tHb) and their integration into an electrochemical array sensing platform. High affinity and specificity aptamers were selected in vitro showing dissociation constants of 2.8 and 2.7 nM for HbA1c and tHb, respectively. Thiol-modified forms of the aptamers were then immobilised on gold nanoparticles (AuNPs)-modified array electrodes and used for the label-free detection of HbA1c and tHb using square wave voltammetry. The voltammetric aptasensors showed high sensitivity with detection limits of 0.2 and 0.34 ng/ml for HbA1c and tHb, respectively. This array platform is superior to the currently available immunoassays in terms of simplicity, stability, ease of use, reduction of sample volume and low cost. Moreover, this method enabled the detection of HbA1c % in human whole blood without any pre-treatment, suggesting great promise of this platform for the diagnosis of diabetes.Shimaa EissaMohammed ZourobNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shimaa Eissa Mohammed Zourob Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood
description	Abstract The increase of the level of glucose in blood leads to an increase in the fraction of glycated hemoglobin (HbA1c). Therefore, the percentage of HbA1c in the blood can serve as a marker for the average glucose level over the past three months and thus, it can be used to diagnose diabetes. Here, we report the selection, identification and characterization of specific DNA aptamers against HbA1c- and total hemoglobin (tHb) and their integration into an electrochemical array sensing platform. High affinity and specificity aptamers were selected in vitro showing dissociation constants of 2.8 and 2.7 nM for HbA1c and tHb, respectively. Thiol-modified forms of the aptamers were then immobilised on gold nanoparticles (AuNPs)-modified array electrodes and used for the label-free detection of HbA1c and tHb using square wave voltammetry. The voltammetric aptasensors showed high sensitivity with detection limits of 0.2 and 0.34 ng/ml for HbA1c and tHb, respectively. This array platform is superior to the currently available immunoassays in terms of simplicity, stability, ease of use, reduction of sample volume and low cost. Moreover, this method enabled the detection of HbA1c % in human whole blood without any pre-treatment, suggesting great promise of this platform for the diagnosis of diabetes.
format	article
author	Shimaa Eissa Mohammed Zourob
author_facet	Shimaa Eissa Mohammed Zourob
author_sort	Shimaa Eissa
title	Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood
title_short	Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood
title_full	Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood
title_fullStr	Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood
title_full_unstemmed	Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood
title_sort	aptamer- based label-free electrochemical biosensor array for the detection of total and glycated hemoglobin in human whole blood
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/973ca5ce414745bbabf7c39c100de13d
work_keys_str_mv	AT shimaaeissa aptamerbasedlabelfreeelectrochemicalbiosensorarrayforthedetectionoftotalandglycatedhemoglobininhumanwholeblood AT mohammedzourob aptamerbasedlabelfreeelectrochemicalbiosensorarrayforthedetectionoftotalandglycatedhemoglobininhumanwholeblood
_version_	1718394879936036864

Aptamer- Based Label-Free Electrochemical Biosensor Array for the Detection of Total and Glycated Hemoglobin in Human Whole Blood

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