Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Abstract Antifungal activity of Monotheca buxifolia methanolic extract and its various fractions were assessed against Macrophomina phaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methano...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shabnam Javed, Zaid Mahmood, Khalid Mohammed Khan, Satyajit D. Sarker, Arshad Javaid, Iqra Haider Khan, Amna Shoaib
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/973d9be2e5894723ba84c9e8f7043075
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:973d9be2e5894723ba84c9e8f7043075
record_format	dspace
spelling	oai:doaj.org-article:973d9be2e5894723ba84c9e8f70430752021-12-02T15:27:12ZLupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina10.1038/s41598-021-87725-72045-2322https://doaj.org/article/973d9be2e5894723ba84c9e8f70430752021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87725-7https://doaj.org/toc/2045-2322Abstract Antifungal activity of Monotheca buxifolia methanolic extract and its various fractions were assessed against Macrophomina phaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methanolic extract (3.125 to 200 mg mL−1) inhibited fungal biomass by 39–45%. Isolated n-hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32–52%, 29–50% and 29–35%, respectively. Triterpenes lupeol and lupeol acetate (1, 2) were isolated from n-hexane while betulin, β-sitosterol, β-amyrin, oleanolic acid (3–6) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid, kaempferol and quercetin (7–10) were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2 mg mL−1) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83–85%. Among the isolated compounds lupeol acetate (2) was found the highest antifungal against M. phaseolina followed by betulin (3), vanillic acid (7), protocatechuic acid (8), β-amyrin (5) and oleanolic acid (6) resulting in 79–81%, 77–79%, 74–79%, 67–72%, 68–71% and 68–71%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced M. phaseolina biomass by 41–64%.Shabnam JavedZaid MahmoodKhalid Mohammed KhanSatyajit D. SarkerArshad JavaidIqra Haider KhanAmna ShoaibNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shabnam Javed Zaid Mahmood Khalid Mohammed Khan Satyajit D. Sarker Arshad Javaid Iqra Haider Khan Amna Shoaib Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina
description	Abstract Antifungal activity of Monotheca buxifolia methanolic extract and its various fractions were assessed against Macrophomina phaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methanolic extract (3.125 to 200 mg mL−1) inhibited fungal biomass by 39–45%. Isolated n-hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32–52%, 29–50% and 29–35%, respectively. Triterpenes lupeol and lupeol acetate (1, 2) were isolated from n-hexane while betulin, β-sitosterol, β-amyrin, oleanolic acid (3–6) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid, kaempferol and quercetin (7–10) were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2 mg mL−1) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83–85%. Among the isolated compounds lupeol acetate (2) was found the highest antifungal against M. phaseolina followed by betulin (3), vanillic acid (7), protocatechuic acid (8), β-amyrin (5) and oleanolic acid (6) resulting in 79–81%, 77–79%, 74–79%, 67–72%, 68–71% and 68–71%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced M. phaseolina biomass by 41–64%.
format	article
author	Shabnam Javed Zaid Mahmood Khalid Mohammed Khan Satyajit D. Sarker Arshad Javaid Iqra Haider Khan Amna Shoaib
author_facet	Shabnam Javed Zaid Mahmood Khalid Mohammed Khan Satyajit D. Sarker Arshad Javaid Iqra Haider Khan Amna Shoaib
author_sort	Shabnam Javed
title	Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina
title_short	Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina
title_full	Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina
title_fullStr	Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina
title_full_unstemmed	Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina
title_sort	lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold macrophomina phaseolina
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/973d9be2e5894723ba84c9e8f7043075
work_keys_str_mv	AT shabnamjaved lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina AT zaidmahmood lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina AT khalidmohammedkhan lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina AT satyajitdsarker lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina AT arshadjavaid lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina AT iqrahaiderkhan lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina AT amnashoaib lupeolacetateasapotentantifungalcompoundagainstopportunistichumanandphytopathogenicmoldmacrophominaphaseolina
_version_	1718387218776588288

Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Ejemplares similares