TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.

TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.

<h4>Background and aim</h4>Some research has suggested that miRNA-10a (miR-10a-5p) had an inhibitory function in proliferation and invasion of cancers. Whereas the role of miR-10a-5p in melanoma has not been fully explored. This study aims to confirm LIN28B as the targeted gene of miR-10...

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Autores principales: Haijun Zhu, Mengshi Kang, Xinping Bai
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
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spelling oai:doaj.org-article:97483778fc0449998a5c01fd3ee07ab72021-12-02T20:17:40ZTCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.1932-620310.1371/journal.pone.0255971https://doaj.org/article/97483778fc0449998a5c01fd3ee07ab72021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255971https://doaj.org/toc/1932-6203<h4>Background and aim</h4>Some research has suggested that miRNA-10a (miR-10a-5p) had an inhibitory function in proliferation and invasion of cancers. Whereas the role of miR-10a-5p in melanoma has not been fully explored. This study aims to confirm LIN28B as the targeted gene of miR-10a-5p which was explored in melanoma cells. In addition, upstream regulatory molecule of miR-10a-5p was also investigated in melanoma cells.<h4>Methods</h4>Real-time Quantitative polymerase chain reaction (RT-qPCR) was adopted to analyze miR-10a-5p expression level in melanoma and the normal human epidermal melanocyte cells. Several biological assays were performed to evaluate miR-10a-5p influences on cell proliferation, migration and invasion ability in A375 and B16-F10 cells. Gene prediction of miRNA targeting and a dual luciferase assay were applied to assess miR-10a-5p-targeted LIN28B. Western blot assessed the impacts of miR-10a-5p on the protein expression of LIN28B. Western blot analyzed the TCF21 effects on the expression of LIN28B and RT-qPCR assessed the influence of TCF21 on the expression level of miRNA-10a. In addition, Chromatin Immunoprecipitation (ChIP) Assay and JASPAR databases were employed to explore the regulatory relationship between TCF21 and miR-10a-5p.<h4>Results</h4>We discovered that miR-10a-5p expression was lower in melanoma cells and high expression of miR-10a-5p suppressed the proliferation, migration and invasion abilities of melanoma cells. We also discovered that miR-10a-5p targeted the LIN28B mRNA 3'UTR area and diminished LIN28B protein expression. We found that LIN28B expression was strongly decreased by TCF21 upregulation in the two melanoma cells. The qRT-PCR assay showed that miR-10a-5p expression level was obviously boosted by increased TCF21 expression. The results also demonstrated that TCF21 directly regulated miR-10a-5p at transcript levels.<h4>Conclusion</h4>TCF21 induced miRNA-10a targeting LIN28B could affect the progression and growth of melanoma.Haijun ZhuMengshi KangXinping BaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255971 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Haijun Zhu
Mengshi Kang
Xinping Bai
TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.
description <h4>Background and aim</h4>Some research has suggested that miRNA-10a (miR-10a-5p) had an inhibitory function in proliferation and invasion of cancers. Whereas the role of miR-10a-5p in melanoma has not been fully explored. This study aims to confirm LIN28B as the targeted gene of miR-10a-5p which was explored in melanoma cells. In addition, upstream regulatory molecule of miR-10a-5p was also investigated in melanoma cells.<h4>Methods</h4>Real-time Quantitative polymerase chain reaction (RT-qPCR) was adopted to analyze miR-10a-5p expression level in melanoma and the normal human epidermal melanocyte cells. Several biological assays were performed to evaluate miR-10a-5p influences on cell proliferation, migration and invasion ability in A375 and B16-F10 cells. Gene prediction of miRNA targeting and a dual luciferase assay were applied to assess miR-10a-5p-targeted LIN28B. Western blot assessed the impacts of miR-10a-5p on the protein expression of LIN28B. Western blot analyzed the TCF21 effects on the expression of LIN28B and RT-qPCR assessed the influence of TCF21 on the expression level of miRNA-10a. In addition, Chromatin Immunoprecipitation (ChIP) Assay and JASPAR databases were employed to explore the regulatory relationship between TCF21 and miR-10a-5p.<h4>Results</h4>We discovered that miR-10a-5p expression was lower in melanoma cells and high expression of miR-10a-5p suppressed the proliferation, migration and invasion abilities of melanoma cells. We also discovered that miR-10a-5p targeted the LIN28B mRNA 3'UTR area and diminished LIN28B protein expression. We found that LIN28B expression was strongly decreased by TCF21 upregulation in the two melanoma cells. The qRT-PCR assay showed that miR-10a-5p expression level was obviously boosted by increased TCF21 expression. The results also demonstrated that TCF21 directly regulated miR-10a-5p at transcript levels.<h4>Conclusion</h4>TCF21 induced miRNA-10a targeting LIN28B could affect the progression and growth of melanoma.
format article
author Haijun Zhu
Mengshi Kang
Xinping Bai
author_facet Haijun Zhu
Mengshi Kang
Xinping Bai
author_sort Haijun Zhu
title TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.
title_short TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.
title_full TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.
title_fullStr TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.
title_full_unstemmed TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells.
title_sort tcf21 regulates mir-10a-5p/lin28b signaling to block the proliferation and invasion of melanoma cells.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/97483778fc0449998a5c01fd3ee07ab7
work_keys_str_mv AT haijunzhu tcf21regulatesmir10a5plin28bsignalingtoblocktheproliferationandinvasionofmelanomacells
AT mengshikang tcf21regulatesmir10a5plin28bsignalingtoblocktheproliferationandinvasionofmelanomacells
AT xinpingbai tcf21regulatesmir10a5plin28bsignalingtoblocktheproliferationandinvasionofmelanomacells
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