Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p

Yonghao Feng, 1,* Ying Ge, 2,* Men Wu, 1 Yangmei Xie, 3 Ming Wang, 3 Yinghui Chen, 3 Xiaohong Shi 1 1Department of Endocrinology, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2Department of General Medicine, Community Health Service Center of Shanghai...

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Autores principales:	Feng Y, Ge Y, Wu M, Xie Y, Wang M, Chen Y, Shi X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	diabetic peripheral neuropathy long non-coding rna microrna inflammation Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/974ffe595c974308bfd7e708abcd6b49
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spelling	oai:doaj.org-article:974ffe595c974308bfd7e708abcd6b492021-12-02T04:08:42ZLong Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p1178-7007https://doaj.org/article/974ffe595c974308bfd7e708abcd6b492020-02-01T00:00:00Zhttps://www.dovepress.com/long-noncoding-rnas-regulate-inflammation-in-diabetic-peripheral-neuro-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Yonghao Feng, 1,* Ying Ge, 2,* Men Wu, 1 Yangmei Xie, 3 Ming Wang, 3 Yinghui Chen, 3 Xiaohong Shi 1 1Department of Endocrinology, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2Department of General Medicine, Community Health Service Center of Shanghai Jinshan Industrial Zone, Shanghai 201506, People’s Republic of China; 3Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaohong ShiDepartment of Endocrinology, Jinshan Hospital, Fudan University, No. 1508 Longhang Road, Shanghai 201508, People’s Republic of ChinaTel +86 21-34189990Fax +86 21-57039502Email xiaohongshi@fudan.edu.cnYinghui ChenDepartment of Neurology, Jinshan Hospital, Fudan University, No. 1508, Longhang Road, Shanghai 201508, People’s Republic of ChinaTel +86 21-34189990Fax +86 21-57039502Email yinghuichen@fudan.edu.cnBackground: Long non-coding RNAs (lncRNAs), as competing endogenous RNAs (ceRNAs), can regulate various pathophysiological processes by binding competitively to microRNAs at the post-transcription level. Our previous work demonstrated that miR-146a-5p was lowly expressed in diabetic peripheral neuropathy (DPN) rats. However, the ceRNA network in DPN mediated by lncRNAs and miR-146a-5p remains to be explored.Methods: Two groups of rats (n=4 per group), a type 2 diabetes (T2DM) group and a DPN group, were used in this study. Sciatic nerve conduction velocity (NCV) of each rat was determined at the 6th and the 12th week. LncRNA microarray analysis was performed in the sciatic nerve of DPN and T2DM rats. Based on the TargetScan algorithm and the miRanda database, we determined the differentially expressed (DE) lncRNAs bound to miR-146a-5p. Furthermore, we verified the DE lncRNAs potentially bound to miR-146a-5p by qRT-PCR. The genes targeted by miR-146a-5p were identified by bioinformatics prediction and experimental techniques.Results: We found 413 DE lncRNAs between DPN and T2DM rats (\|log2FC\| ≥ 2 and adjust P ≤ 0.05). Eight DE lncRNAs were predicted to bind to miR-146a-5p by both algorithms, of which four were verified by qRT-PCR. TRAF6, IRAK1, and SMAD4 were identified as miR-146a-5p targeted genes and were predominantly enriched in the inflammatory signaling pathway.Conclusion: LncRNAs may contribute to the pathogenesis of DPN by regulating inflammation through functioning as ceRNAs of miR-146a-5p.Keywords: diabetic peripheral neuropathy, long non-coding RNA, MicroRNA, inflammationFeng YGe YWu MXie YWang MChen YShi XDove Medical Pressarticlediabetic peripheral neuropathylong non-coding rnamicrornainflammationSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 413-422 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic peripheral neuropathy long non-coding rna microrna inflammation Specialties of internal medicine RC581-951
spellingShingle	diabetic peripheral neuropathy long non-coding rna microrna inflammation Specialties of internal medicine RC581-951 Feng Y Ge Y Wu M Xie Y Wang M Chen Y Shi X Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p
description	Yonghao Feng, 1,* Ying Ge, 2,* Men Wu, 1 Yangmei Xie, 3 Ming Wang, 3 Yinghui Chen, 3 Xiaohong Shi 1 1Department of Endocrinology, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2Department of General Medicine, Community Health Service Center of Shanghai Jinshan Industrial Zone, Shanghai 201506, People’s Republic of China; 3Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaohong ShiDepartment of Endocrinology, Jinshan Hospital, Fudan University, No. 1508 Longhang Road, Shanghai 201508, People’s Republic of ChinaTel +86 21-34189990Fax +86 21-57039502Email xiaohongshi@fudan.edu.cnYinghui ChenDepartment of Neurology, Jinshan Hospital, Fudan University, No. 1508, Longhang Road, Shanghai 201508, People’s Republic of ChinaTel +86 21-34189990Fax +86 21-57039502Email yinghuichen@fudan.edu.cnBackground: Long non-coding RNAs (lncRNAs), as competing endogenous RNAs (ceRNAs), can regulate various pathophysiological processes by binding competitively to microRNAs at the post-transcription level. Our previous work demonstrated that miR-146a-5p was lowly expressed in diabetic peripheral neuropathy (DPN) rats. However, the ceRNA network in DPN mediated by lncRNAs and miR-146a-5p remains to be explored.Methods: Two groups of rats (n=4 per group), a type 2 diabetes (T2DM) group and a DPN group, were used in this study. Sciatic nerve conduction velocity (NCV) of each rat was determined at the 6th and the 12th week. LncRNA microarray analysis was performed in the sciatic nerve of DPN and T2DM rats. Based on the TargetScan algorithm and the miRanda database, we determined the differentially expressed (DE) lncRNAs bound to miR-146a-5p. Furthermore, we verified the DE lncRNAs potentially bound to miR-146a-5p by qRT-PCR. The genes targeted by miR-146a-5p were identified by bioinformatics prediction and experimental techniques.Results: We found 413 DE lncRNAs between DPN and T2DM rats (\|log2FC\| ≥ 2 and adjust P ≤ 0.05). Eight DE lncRNAs were predicted to bind to miR-146a-5p by both algorithms, of which four were verified by qRT-PCR. TRAF6, IRAK1, and SMAD4 were identified as miR-146a-5p targeted genes and were predominantly enriched in the inflammatory signaling pathway.Conclusion: LncRNAs may contribute to the pathogenesis of DPN by regulating inflammation through functioning as ceRNAs of miR-146a-5p.Keywords: diabetic peripheral neuropathy, long non-coding RNA, MicroRNA, inflammation
format	article
author	Feng Y Ge Y Wu M Xie Y Wang M Chen Y Shi X
author_facet	Feng Y Ge Y Wu M Xie Y Wang M Chen Y Shi X
author_sort	Feng Y
title	Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p
title_short	Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p
title_full	Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p
title_fullStr	Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p
title_full_unstemmed	Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p
title_sort	long non-coding rnas regulate inflammation in diabetic peripheral neuropathy by acting as cernas targeting mir-146a-5p
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/974ffe595c974308bfd7e708abcd6b49
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Long Non-Coding RNAs Regulate Inflammation in Diabetic Peripheral Neuropathy by Acting as ceRNAs Targeting miR-146a-5p

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