Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering

Tonghe Zhu,1 Jia Jiang,1 Jinzhong Zhao,1 Sihao Chen,2,3 Xiaoyu Yan1 1Department of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, People’s Republic of China; 2Scientific Research Department of Shangha...

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Autores principales:	Zhu T, Jiang J, Zhao J, Chen S, Yan X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	three-dimensional scaffold flurbiprofen freeze-drying amidation skin tissue engineering Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/97523600bd0a40599fd030f0087244f4
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spelling	oai:doaj.org-article:97523600bd0a40599fd030f0087244f42021-12-02T08:53:47ZRegulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering1178-2013https://doaj.org/article/97523600bd0a40599fd030f0087244f42019-11-01T00:00:00Zhttps://www.dovepress.com/regulating-preparation-of-functional-alginate-chitosan-three-dimension-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tonghe Zhu,1 Jia Jiang,1 Jinzhong Zhao,1 Sihao Chen,2,3 Xiaoyu Yan1 1Department of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, People’s Republic of China; 2Scientific Research Department of Shanghai University of Engineering Science, Shanghai 201620, People’s Republic of China; 3Multidisciplinary Center for Advanced Materials of Shanghai University of Engineering Science, Shanghai University of Engineering Science, Shanghai 201620, People’s Republic of ChinaCorrespondence: Xiaoyu YanDepartment of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, No. 600 Yishan Road, Shanghai 200233, People’s Republic of ChinaTel/Fax +86 21 24056088Email xyyan@sjtu.edu.cnAim: In this study, we attempted to regulate the preparation of Alg-CS-Flu three-dimensional scaffolds via a facile freeze-drying method combined with amidation.Materials and methods: Three-dimensional porous flurbiprofen-grafted alginate (Alg)–chitosan (CS) scaffolds were successfully prepared by a facile freeze-drying method combined with amidation for skin tissue engineering applications. Alg-CS composite was first used to load flurbiprofen (Flu), which is a kind of anti-inflammatory non-steroidal molecule. The Flu-loaded Alg/CS composite solution, through freeze-drying and 1-ethyl-3(3-(dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide crosslinking to form an Alg-CS-Flu scaffold, exhibited a uniform and porous morphology that was characterized using scanning electron microscopy. The Alg-CS-Flu as-prepared scaffold was also characterized using Fourier-transform infrared spectroscopy, water contact angle, thermal properties, and stress-strain testing.Results: The results reveal that Flu was successfully grafted onto the surfaces of the Alg-CS-Flu scaffold, which showed good hydrophilicity and appropriate mechanical properties. Furthermore, cell viability, cell morphology from cells cultured in vitro, and hematoxylin-eosin staining after the graft was subcutaneously embedded in mice for 7 d demonstrated that the Alg-CS-Flu scaffold had no unfavorable effects on the adhesion and proliferation of fibroblasts, as well as a good anti-inflammatory property.Conclusion: The developed Alg-CS-Flu scaffold is proposed as a promising material or skin tissue engineering application.Keywords: three-dimensional scaffold, flurbiprofen, freeze-drying, amidation, skin tissue engineeringZhu TJiang JZhao JChen SYan XDove Medical Pressarticlethree-dimensional scaffoldflurbiprofenfreeze-dryingamidationskin tissue engineeringMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8891-8903 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	three-dimensional scaffold flurbiprofen freeze-drying amidation skin tissue engineering Medicine (General) R5-920
spellingShingle	three-dimensional scaffold flurbiprofen freeze-drying amidation skin tissue engineering Medicine (General) R5-920 Zhu T Jiang J Zhao J Chen S Yan X Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
description	Tonghe Zhu,1 Jia Jiang,1 Jinzhong Zhao,1 Sihao Chen,2,3 Xiaoyu Yan1 1Department of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, People’s Republic of China; 2Scientific Research Department of Shanghai University of Engineering Science, Shanghai 201620, People’s Republic of China; 3Multidisciplinary Center for Advanced Materials of Shanghai University of Engineering Science, Shanghai University of Engineering Science, Shanghai 201620, People’s Republic of ChinaCorrespondence: Xiaoyu YanDepartment of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, No. 600 Yishan Road, Shanghai 200233, People’s Republic of ChinaTel/Fax +86 21 24056088Email xyyan@sjtu.edu.cnAim: In this study, we attempted to regulate the preparation of Alg-CS-Flu three-dimensional scaffolds via a facile freeze-drying method combined with amidation.Materials and methods: Three-dimensional porous flurbiprofen-grafted alginate (Alg)–chitosan (CS) scaffolds were successfully prepared by a facile freeze-drying method combined with amidation for skin tissue engineering applications. Alg-CS composite was first used to load flurbiprofen (Flu), which is a kind of anti-inflammatory non-steroidal molecule. The Flu-loaded Alg/CS composite solution, through freeze-drying and 1-ethyl-3(3-(dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide crosslinking to form an Alg-CS-Flu scaffold, exhibited a uniform and porous morphology that was characterized using scanning electron microscopy. The Alg-CS-Flu as-prepared scaffold was also characterized using Fourier-transform infrared spectroscopy, water contact angle, thermal properties, and stress-strain testing.Results: The results reveal that Flu was successfully grafted onto the surfaces of the Alg-CS-Flu scaffold, which showed good hydrophilicity and appropriate mechanical properties. Furthermore, cell viability, cell morphology from cells cultured in vitro, and hematoxylin-eosin staining after the graft was subcutaneously embedded in mice for 7 d demonstrated that the Alg-CS-Flu scaffold had no unfavorable effects on the adhesion and proliferation of fibroblasts, as well as a good anti-inflammatory property.Conclusion: The developed Alg-CS-Flu scaffold is proposed as a promising material or skin tissue engineering application.Keywords: three-dimensional scaffold, flurbiprofen, freeze-drying, amidation, skin tissue engineering
format	article
author	Zhu T Jiang J Zhao J Chen S Yan X
author_facet	Zhu T Jiang J Zhao J Chen S Yan X
author_sort	Zhu T
title	Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
title_short	Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
title_full	Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
title_fullStr	Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
title_full_unstemmed	Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
title_sort	regulating preparation of functional alginate-chitosan three-dimensional scaffold for skin tissue engineering
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/97523600bd0a40599fd030f0087244f4
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Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering

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