Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione

SV Sheen Mers,1,2 Elumalai Thambuswamy Deva Kumar,1 V Ganesh1,2 1Electrodics and Electrocatalysis (EEC) Division, Council of Scientific and Industrial Research–Central Electrochemical Research Institute (CSIR–CECRI), Karaikudi, Tamil Nadu, India; 2Academy of Scientific and Innov...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sheen Mers SV, Deva Kumar ET, Ganesh V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9757042aecf243a8a4c4155549ada3e5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9757042aecf243a8a4c4155549ada3e5
record_format	dspace
spelling	oai:doaj.org-article:9757042aecf243a8a4c4155549ada3e52021-12-02T06:34:33ZGold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione1178-2013https://doaj.org/article/9757042aecf243a8a4c4155549ada3e52015-10-01T00:00:00Zhttps://www.dovepress.com/gold-nanoparticles-immobilized-hierarchically-ordered-porous-tio2-nano-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013SV Sheen Mers,1,2 Elumalai Thambuswamy Deva Kumar,1 V Ganesh1,2 1Electrodics and Electrocatalysis (EEC) Division, Council of Scientific and Industrial Research–Central Electrochemical Research Institute (CSIR–CECRI), Karaikudi, Tamil Nadu, India; 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India Abstract: Glutathione (GSH) is vital for several functions of our human body such as neutralization of free radicals and reactive oxygen compounds, maintaining the active forms of vitamin C and E, regulation of nitric oxide cycle, iron metabolism, etc. It is also an endogenous antioxidant in most of the biological reactions. Given the importance of GSH, a simple strategy is proposed in this work to develop a biosensor for quantitative detection of GSH. This particular biosensor comprises of gold nanoparticles (Au NPs)-immobilized, hierarchically ordered titanium dioxide (TiO2) porous nanotubes. Hexagonally arranged, honeycomb-like nanoporous tubular TiO2 electrodes are prepared by using a simple electrochemical anodization process by applying a constant potential of 30 V for 24 hours using ethylene glycol consisting of ammonium fluoride as an electrolytic medium. Structural morphology and crystalline nature of such TiO2 nanotubes are analyzed using field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD). Interestingly, nanocomposites of TiO2 with Au NPs is prepared in an effort to alter the intrinsic properties of TiO2, especially tuning of its band gap. Au NPs are prepared by a well-known Brust and Schiffrin method and are immobilized onto TiO2 electrodes which act as a perfect electrochemical sensing platform for GSH detection. Structural characterization and analysis of these modified electrodes are performed using FESEM, XRD, and UV-visible spectroscopic studies. GSH binding events on Au NPs-immobilized porous TiO2 electrodes are monitored by electrochemical techniques, namely, cyclic voltammetry (CV) and chronoamperometry (CA). Several parameters such as sensitivity, selectivity, stability, limit of detection, etc are investigated. In addition, Au NPs dispersed in aqueous medium are also explored for naked-eye detection of GSH using UV-visible spectroscopy in order to compare the performance of the proposed sensor. Our studies clearly indicate that these materials could potentially be used for GSH sensing applications. Keywords: biosensor, electrochemistry, glutathione, gold nanoparticles, TiO2 nanotubesSheen Mers SVDeva Kumar ETGanesh VDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Supplement 1 Challenges in biomaterials research, Pp 171-182 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sheen Mers SV Deva Kumar ET Ganesh V Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione
description	SV Sheen Mers,1,2 Elumalai Thambuswamy Deva Kumar,1 V Ganesh1,2 1Electrodics and Electrocatalysis (EEC) Division, Council of Scientific and Industrial Research–Central Electrochemical Research Institute (CSIR–CECRI), Karaikudi, Tamil Nadu, India; 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India Abstract: Glutathione (GSH) is vital for several functions of our human body such as neutralization of free radicals and reactive oxygen compounds, maintaining the active forms of vitamin C and E, regulation of nitric oxide cycle, iron metabolism, etc. It is also an endogenous antioxidant in most of the biological reactions. Given the importance of GSH, a simple strategy is proposed in this work to develop a biosensor for quantitative detection of GSH. This particular biosensor comprises of gold nanoparticles (Au NPs)-immobilized, hierarchically ordered titanium dioxide (TiO2) porous nanotubes. Hexagonally arranged, honeycomb-like nanoporous tubular TiO2 electrodes are prepared by using a simple electrochemical anodization process by applying a constant potential of 30 V for 24 hours using ethylene glycol consisting of ammonium fluoride as an electrolytic medium. Structural morphology and crystalline nature of such TiO2 nanotubes are analyzed using field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD). Interestingly, nanocomposites of TiO2 with Au NPs is prepared in an effort to alter the intrinsic properties of TiO2, especially tuning of its band gap. Au NPs are prepared by a well-known Brust and Schiffrin method and are immobilized onto TiO2 electrodes which act as a perfect electrochemical sensing platform for GSH detection. Structural characterization and analysis of these modified electrodes are performed using FESEM, XRD, and UV-visible spectroscopic studies. GSH binding events on Au NPs-immobilized porous TiO2 electrodes are monitored by electrochemical techniques, namely, cyclic voltammetry (CV) and chronoamperometry (CA). Several parameters such as sensitivity, selectivity, stability, limit of detection, etc are investigated. In addition, Au NPs dispersed in aqueous medium are also explored for naked-eye detection of GSH using UV-visible spectroscopy in order to compare the performance of the proposed sensor. Our studies clearly indicate that these materials could potentially be used for GSH sensing applications. Keywords: biosensor, electrochemistry, glutathione, gold nanoparticles, TiO2 nanotubes
format	article
author	Sheen Mers SV Deva Kumar ET Ganesh V
author_facet	Sheen Mers SV Deva Kumar ET Ganesh V
author_sort	Sheen Mers SV
title	Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione
title_short	Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione
title_full	Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione
title_fullStr	Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione
title_full_unstemmed	Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione
title_sort	gold nanoparticles-immobilized, hierarchically ordered, porous tio2 nanotubes for biosensing of glutathione
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/9757042aecf243a8a4c4155549ada3e5
work_keys_str_mv	AT sheenmerssv goldnanoparticlesimmobilizedhierarchicallyorderedporoustio2nanotubesforbiosensingofglutathione AT devakumaret goldnanoparticlesimmobilizedhierarchicallyorderedporoustio2nanotubesforbiosensingofglutathione AT ganeshv goldnanoparticlesimmobilizedhierarchicallyorderedporoustio2nanotubesforbiosensingofglutathione
_version_	1718399827409108992

Gold nanoparticles-immobilized, hierarchically ordered, porous TiO2 nanotubes for biosensing of glutathione

Ejemplares similares