Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength

Abstract Pannexins (Panxs), glycoproteins that oligomerize to form hemichannels on the cell membrane, are topologically similar to connexins, but do not form cell-to-cell gap junction channels. There are 3 members of the family, 1–3, with Panx1 being the most abundant. All Panxs are expressed in bon...

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Autores principales:	Alexandra Aguilar-Perez, Rafael Pacheco-Costa, Emily G. Atkinson, Padmini Deosthale, Hannah M. Davis, Alyson L. Essex, Julian E. Dilley, Leland Gomez, Joseph E. Rupert, Teresa A. Zimmers, Roger J. Thompson, Matthew R. Allen, Lilian I. Plotkin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97573a8376fe40a69c68de24c809d8bf
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spelling	oai:doaj.org-article:97573a8376fe40a69c68de24c809d8bf2021-12-02T15:08:59ZAge- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength10.1038/s41598-019-50444-12045-2322https://doaj.org/article/97573a8376fe40a69c68de24c809d8bf2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50444-1https://doaj.org/toc/2045-2322Abstract Pannexins (Panxs), glycoproteins that oligomerize to form hemichannels on the cell membrane, are topologically similar to connexins, but do not form cell-to-cell gap junction channels. There are 3 members of the family, 1–3, with Panx1 being the most abundant. All Panxs are expressed in bone, but their role in bone cell biology is not completely understood. We now report that osteocytic Panx1 deletion (Panx1Δot) alters bone mass and strength in female mice. Bone mineral density after reaching skeletal maturity is higher in female Panx1Δot mice than in control Panx1fl/fl mice. Further, osteocytic Panx1 deletion partially prevented aging effects on cortical bone structure and mechanical properties. Young 4-month-old female Panx1Δot mice exhibited increased lean body mass, even though pannexin levels in skeletal muscle were not affected; whereas no difference in lean body mass was detected in male mice. Furthermore, female Panx1-deficient mice exhibited increased muscle mass without changes in strength, whereas Panx1Δot males showed unchanged muscle mass and decreased in vivo maximum plantarflexion torque, indicating reduced muscle strength. Our results suggest that osteocytic Panx1 deletion increases bone mass in young and old female mice and muscle mass in young female mice, but has deleterious effects on muscle strength only in males.Alexandra Aguilar-PerezRafael Pacheco-CostaEmily G. AtkinsonPadmini DeosthaleHannah M. DavisAlyson L. EssexJulian E. DilleyLeland GomezJoseph E. RupertTeresa A. ZimmersRoger J. ThompsonMatthew R. AllenLilian I. PlotkinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Aguilar-Perez Rafael Pacheco-Costa Emily G. Atkinson Padmini Deosthale Hannah M. Davis Alyson L. Essex Julian E. Dilley Leland Gomez Joseph E. Rupert Teresa A. Zimmers Roger J. Thompson Matthew R. Allen Lilian I. Plotkin Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
description	Abstract Pannexins (Panxs), glycoproteins that oligomerize to form hemichannels on the cell membrane, are topologically similar to connexins, but do not form cell-to-cell gap junction channels. There are 3 members of the family, 1–3, with Panx1 being the most abundant. All Panxs are expressed in bone, but their role in bone cell biology is not completely understood. We now report that osteocytic Panx1 deletion (Panx1Δot) alters bone mass and strength in female mice. Bone mineral density after reaching skeletal maturity is higher in female Panx1Δot mice than in control Panx1fl/fl mice. Further, osteocytic Panx1 deletion partially prevented aging effects on cortical bone structure and mechanical properties. Young 4-month-old female Panx1Δot mice exhibited increased lean body mass, even though pannexin levels in skeletal muscle were not affected; whereas no difference in lean body mass was detected in male mice. Furthermore, female Panx1-deficient mice exhibited increased muscle mass without changes in strength, whereas Panx1Δot males showed unchanged muscle mass and decreased in vivo maximum plantarflexion torque, indicating reduced muscle strength. Our results suggest that osteocytic Panx1 deletion increases bone mass in young and old female mice and muscle mass in young female mice, but has deleterious effects on muscle strength only in males.
format	article
author	Alexandra Aguilar-Perez Rafael Pacheco-Costa Emily G. Atkinson Padmini Deosthale Hannah M. Davis Alyson L. Essex Julian E. Dilley Leland Gomez Joseph E. Rupert Teresa A. Zimmers Roger J. Thompson Matthew R. Allen Lilian I. Plotkin
author_facet	Alexandra Aguilar-Perez Rafael Pacheco-Costa Emily G. Atkinson Padmini Deosthale Hannah M. Davis Alyson L. Essex Julian E. Dilley Leland Gomez Joseph E. Rupert Teresa A. Zimmers Roger J. Thompson Matthew R. Allen Lilian I. Plotkin
author_sort	Alexandra Aguilar-Perez
title	Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
title_short	Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
title_full	Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
title_fullStr	Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
title_full_unstemmed	Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
title_sort	age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/97573a8376fe40a69c68de24c809d8bf
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Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength

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