High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange

Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell reperto...

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Autores principales:	Sarah A. Overall, Jugmohit S. Toor, Stephanie Hao, Mark Yarmarkovich, Sara M. O’Rourke, Giora I. Morozov, Son Nguyen, Alberto Sada Japp, Nicolas Gonzalez, Danai Moschidi, Michael R. Betts, John M. Maris, Peter Smibert, Nikolaos G. Sgourakis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/975b9e150895476f87a40ab18d6ce573
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spelling	oai:doaj.org-article:975b9e150895476f87a40ab18d6ce5732021-12-02T14:41:15ZHigh throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange10.1038/s41467-020-15710-12041-1723https://doaj.org/article/975b9e150895476f87a40ab18d6ce5732020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15710-1https://doaj.org/toc/2041-1723Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.Sarah A. OverallJugmohit S. ToorStephanie HaoMark YarmarkovichSara M. O’RourkeGiora I. MorozovSon NguyenAlberto Sada JappNicolas GonzalezDanai MoschidiMichael R. BettsJohn M. MarisPeter SmibertNikolaos G. SgourakisNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sarah A. Overall Jugmohit S. Toor Stephanie Hao Mark Yarmarkovich Sara M. O’Rourke Giora I. Morozov Son Nguyen Alberto Sada Japp Nicolas Gonzalez Danai Moschidi Michael R. Betts John M. Maris Peter Smibert Nikolaos G. Sgourakis High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
description	Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.
format	article
author	Sarah A. Overall Jugmohit S. Toor Stephanie Hao Mark Yarmarkovich Sara M. O’Rourke Giora I. Morozov Son Nguyen Alberto Sada Japp Nicolas Gonzalez Danai Moschidi Michael R. Betts John M. Maris Peter Smibert Nikolaos G. Sgourakis
author_facet	Sarah A. Overall Jugmohit S. Toor Stephanie Hao Mark Yarmarkovich Sara M. O’Rourke Giora I. Morozov Son Nguyen Alberto Sada Japp Nicolas Gonzalez Danai Moschidi Michael R. Betts John M. Maris Peter Smibert Nikolaos G. Sgourakis
author_sort	Sarah A. Overall
title	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_short	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_full	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_fullStr	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_full_unstemmed	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_sort	high throughput pmhc-i tetramer library production using chaperone-mediated peptide exchange
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/975b9e150895476f87a40ab18d6ce573
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High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange

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