Optical Control of Tumor Induction in the Zebrafish

Abstract The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhiping Feng, Suzy Nam, Fatima Hamouri, Isabelle Aujard, Bertrand Ducos, Sophie Vriz, Michel Volovitch, Ludovic Jullien, Shuo Lin, Shimon Weiss, David Bensimon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/975e7ac1b88d4eb99800287100377942
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:975e7ac1b88d4eb99800287100377942
record_format	dspace
spelling	oai:doaj.org-article:975e7ac1b88d4eb998002871003779422021-12-02T15:06:00ZOptical Control of Tumor Induction in the Zebrafish10.1038/s41598-017-09697-x2045-2322https://doaj.org/article/975e7ac1b88d4eb998002871003779422017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09697-xhttps://doaj.org/toc/2045-2322Abstract The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells.Zhiping FengSuzy NamFatima HamouriIsabelle AujardBertrand DucosSophie VrizMichel VolovitchLudovic JullienShuo LinShimon WeissDavid BensimonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiping Feng Suzy Nam Fatima Hamouri Isabelle Aujard Bertrand Ducos Sophie Vriz Michel Volovitch Ludovic Jullien Shuo Lin Shimon Weiss David Bensimon Optical Control of Tumor Induction in the Zebrafish
description	Abstract The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells.
format	article
author	Zhiping Feng Suzy Nam Fatima Hamouri Isabelle Aujard Bertrand Ducos Sophie Vriz Michel Volovitch Ludovic Jullien Shuo Lin Shimon Weiss David Bensimon
author_facet	Zhiping Feng Suzy Nam Fatima Hamouri Isabelle Aujard Bertrand Ducos Sophie Vriz Michel Volovitch Ludovic Jullien Shuo Lin Shimon Weiss David Bensimon
author_sort	Zhiping Feng
title	Optical Control of Tumor Induction in the Zebrafish
title_short	Optical Control of Tumor Induction in the Zebrafish
title_full	Optical Control of Tumor Induction in the Zebrafish
title_fullStr	Optical Control of Tumor Induction in the Zebrafish
title_full_unstemmed	Optical Control of Tumor Induction in the Zebrafish
title_sort	optical control of tumor induction in the zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/975e7ac1b88d4eb99800287100377942
work_keys_str_mv	AT zhipingfeng opticalcontroloftumorinductioninthezebrafish AT suzynam opticalcontroloftumorinductioninthezebrafish AT fatimahamouri opticalcontroloftumorinductioninthezebrafish AT isabelleaujard opticalcontroloftumorinductioninthezebrafish AT bertrandducos opticalcontroloftumorinductioninthezebrafish AT sophievriz opticalcontroloftumorinductioninthezebrafish AT michelvolovitch opticalcontroloftumorinductioninthezebrafish AT ludovicjullien opticalcontroloftumorinductioninthezebrafish AT shuolin opticalcontroloftumorinductioninthezebrafish AT shimonweiss opticalcontroloftumorinductioninthezebrafish AT davidbensimon opticalcontroloftumorinductioninthezebrafish
_version_	1718388659751747584

Optical Control of Tumor Induction in the Zebrafish

Ejemplares similares