The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways

Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regula...

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Autores principales:	Chunlian Tian, Xiaoyun Chen, Yu Chang, Ruxia Wang, Jing Ning, Cancan Cui, Mingchun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	anti-inflammatory effect regulatory mechanism total flavonoid extract quantitative real-time pcr abutilon theophrasti Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9762d69b05a5410c82146ebd5c831e0c
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spelling	oai:doaj.org-article:9762d69b05a5410c82146ebd5c831e0c2021-11-17T14:21:56ZThe regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways1388-02091744-511610.1080/13880209.2019.1648523https://doaj.org/article/9762d69b05a5410c82146ebd5c831e0c2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1648523https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regulatory effect of a TFE from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways. Materials and methods: Kunming mice were intragastrically administered TFE (0.25, 0.5, 1.0 g/kg) for 5 days, and then ALI was induced via intranasal administration 40 μg of LPS in 10 μL PBS after intragastric administration on the 5th day, and PBS and DEX (2 mg/kg) were negative and positive control groups, respectively. Results: The relative expression of iNOS gene was 0.707, 0.507 and 0.483 for 0.25, 0.5 and 1.0 g/kg TFE, and COX-2 gene expression was also reduced after treatment by three concentrations of TFE with 0.768, 0.545, and 0.478. The mRNA expression levels of p65 were 0.61, 0.43 and 0.27 for 0.25, 0.5 and 1.0 g/kg TFE and IκB levels were increased in a dose-dependent manner with 3.99, 13.69 and 34.36. 0.5 and 1.0 g/kg TFE inhibited the expression of ERK1/2 with 0.59 and 0.38, p38MAPK with 0.62 and 0.54, and JNK with 0.37 and 0.29, and JNK mRNA expression was 0.60 for 0.25 g/kg TFE. Discussion and conclusion: These results indicate that the regulatory mechanisms of TFE on gene expression in LPS-induced ALI mice include inhibition of the NF-κB and MAPK signaling pathways.Chunlian TianXiaoyun ChenYu ChangRuxia WangJing NingCancan CuiMingchun LiuTaylor & Francis Grouparticleanti-inflammatory effectregulatory mechanismtotal flavonoid extractquantitative real-time pcrabutilon theophrastiTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 514-518 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-inflammatory effect regulatory mechanism total flavonoid extract quantitative real-time pcr abutilon theophrasti Therapeutics. Pharmacology RM1-950
spellingShingle	anti-inflammatory effect regulatory mechanism total flavonoid extract quantitative real-time pcr abutilon theophrasti Therapeutics. Pharmacology RM1-950 Chunlian Tian Xiaoyun Chen Yu Chang Ruxia Wang Jing Ning Cancan Cui Mingchun Liu The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways
description	Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regulatory effect of a TFE from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways. Materials and methods: Kunming mice were intragastrically administered TFE (0.25, 0.5, 1.0 g/kg) for 5 days, and then ALI was induced via intranasal administration 40 μg of LPS in 10 μL PBS after intragastric administration on the 5th day, and PBS and DEX (2 mg/kg) were negative and positive control groups, respectively. Results: The relative expression of iNOS gene was 0.707, 0.507 and 0.483 for 0.25, 0.5 and 1.0 g/kg TFE, and COX-2 gene expression was also reduced after treatment by three concentrations of TFE with 0.768, 0.545, and 0.478. The mRNA expression levels of p65 were 0.61, 0.43 and 0.27 for 0.25, 0.5 and 1.0 g/kg TFE and IκB levels were increased in a dose-dependent manner with 3.99, 13.69 and 34.36. 0.5 and 1.0 g/kg TFE inhibited the expression of ERK1/2 with 0.59 and 0.38, p38MAPK with 0.62 and 0.54, and JNK with 0.37 and 0.29, and JNK mRNA expression was 0.60 for 0.25 g/kg TFE. Discussion and conclusion: These results indicate that the regulatory mechanisms of TFE on gene expression in LPS-induced ALI mice include inhibition of the NF-κB and MAPK signaling pathways.
format	article
author	Chunlian Tian Xiaoyun Chen Yu Chang Ruxia Wang Jing Ning Cancan Cui Mingchun Liu
author_facet	Chunlian Tian Xiaoyun Chen Yu Chang Ruxia Wang Jing Ning Cancan Cui Mingchun Liu
author_sort	Chunlian Tian
title	The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways
title_short	The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways
title_full	The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways
title_fullStr	The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways
title_full_unstemmed	The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways
title_sort	regulatory effect of flavonoids extracted from abutilon theophrasti leaves on gene expression in lps-induced ali mice via the nf-κb and mapk signaling pathways
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/9762d69b05a5410c82146ebd5c831e0c
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The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways

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