Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.

Cultured epidermal cell sheets (CECS) are used in regenerative medicine in patients with burns, and have potential to treat limbal stem cell deficiency (LSCD), as demonstrated in animal models. Despite widespread use, short-term storage options for CECS are limited. Advantages of storage include: fl...

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Autores principales:	Catherine Jackson, Peder Aabel, Jon R Eidet, Edward B Messelt, Torstein Lyberg, Magnus von Unge, Tor P Utheim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97672eb00bee41e88c462d91e4a053c9
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spelling	oai:doaj.org-article:97672eb00bee41e88c462d91e4a053c92021-11-25T06:02:30ZEffect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.1932-620310.1371/journal.pone.0105808https://doaj.org/article/97672eb00bee41e88c462d91e4a053c92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25170754/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cultured epidermal cell sheets (CECS) are used in regenerative medicine in patients with burns, and have potential to treat limbal stem cell deficiency (LSCD), as demonstrated in animal models. Despite widespread use, short-term storage options for CECS are limited. Advantages of storage include: flexibility in scheduling surgery, reserve sheets for repeat operations, more opportunity for quality control, and improved transportation to allow wider distribution. Studies on storage of CECS have thus far focused on cryopreservation, whereas refrigeration is a convenient method commonly used for whole skin graft storage in burns clinics. It has been shown that preservation of viable cells using these methods is variable. This study evaluated the effect of different temperatures spanning 4°C to 37°C, on the cell viability, morphology, proliferation and metabolic status of CECS stored over a two week period in a xenobiotic-free system. Compared to non-stored control, best cell viability was obtained at 24°C (95.2±9.9%); reduced cell viability, at approximately 60%, was demonstrated at several of the temperatures (12°C, 28°C, 32°C and 37°C). Metabolic activity was significantly higher between 24°C and 37°C, where glucose, lactate, lactate/glucose ratios, and oxygen tension indicated increased activation of the glycolytic pathway under aerobic conditions. Preservation of morphology as shown by phase contrast and scanning electron micrographs was best at 12°C and 16°C. PCNA immunocytochemistry indicated that only 12°C and 20°C allowed maintenance of proliferative function at a similar level to non-stored control. In conclusion, results indicate that 12°C and 24°C merit further investigation as the prospective optimum temperature for short-term storage of cultured epidermal cell sheets.Catherine JacksonPeder AabelJon R EidetEdward B MesseltTorstein LybergMagnus von UngeTor P UtheimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105808 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Catherine Jackson Peder Aabel Jon R Eidet Edward B Messelt Torstein Lyberg Magnus von Unge Tor P Utheim Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
description	Cultured epidermal cell sheets (CECS) are used in regenerative medicine in patients with burns, and have potential to treat limbal stem cell deficiency (LSCD), as demonstrated in animal models. Despite widespread use, short-term storage options for CECS are limited. Advantages of storage include: flexibility in scheduling surgery, reserve sheets for repeat operations, more opportunity for quality control, and improved transportation to allow wider distribution. Studies on storage of CECS have thus far focused on cryopreservation, whereas refrigeration is a convenient method commonly used for whole skin graft storage in burns clinics. It has been shown that preservation of viable cells using these methods is variable. This study evaluated the effect of different temperatures spanning 4°C to 37°C, on the cell viability, morphology, proliferation and metabolic status of CECS stored over a two week period in a xenobiotic-free system. Compared to non-stored control, best cell viability was obtained at 24°C (95.2±9.9%); reduced cell viability, at approximately 60%, was demonstrated at several of the temperatures (12°C, 28°C, 32°C and 37°C). Metabolic activity was significantly higher between 24°C and 37°C, where glucose, lactate, lactate/glucose ratios, and oxygen tension indicated increased activation of the glycolytic pathway under aerobic conditions. Preservation of morphology as shown by phase contrast and scanning electron micrographs was best at 12°C and 16°C. PCNA immunocytochemistry indicated that only 12°C and 20°C allowed maintenance of proliferative function at a similar level to non-stored control. In conclusion, results indicate that 12°C and 24°C merit further investigation as the prospective optimum temperature for short-term storage of cultured epidermal cell sheets.
format	article
author	Catherine Jackson Peder Aabel Jon R Eidet Edward B Messelt Torstein Lyberg Magnus von Unge Tor P Utheim
author_facet	Catherine Jackson Peder Aabel Jon R Eidet Edward B Messelt Torstein Lyberg Magnus von Unge Tor P Utheim
author_sort	Catherine Jackson
title	Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
title_short	Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
title_full	Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
title_fullStr	Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
title_full_unstemmed	Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
title_sort	effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/97672eb00bee41e88c462d91e4a053c9
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Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.

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