Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae

Abstract Mosquito-borne diseases cause one million deaths and hundreds of millions of human infections yearly. With all such diseases, the pathogen must traverse the mosquito salivary gland (SG) for transmission to a new host, making the SGs ideal targets for genetic strategies to block transmission...

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Autores principales:	Michael B. Wells, Jordan Villamor, Deborah J. Andrew
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9768154b15284f5dbc3a5a974e185972
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spelling	oai:doaj.org-article:9768154b15284f5dbc3a5a974e1859722021-12-02T11:53:13ZSalivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae10.1038/s41598-017-00672-02045-2322https://doaj.org/article/9768154b15284f5dbc3a5a974e1859722017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00672-0https://doaj.org/toc/2045-2322Abstract Mosquito-borne diseases cause one million deaths and hundreds of millions of human infections yearly. With all such diseases, the pathogen must traverse the mosquito salivary gland (SG) for transmission to a new host, making the SGs ideal targets for genetic strategies to block transmission. Prior studies have elucidated details of SG structure by light and electron microscopy and have deeply explored the salivary transcriptome and proteome. Very little is known, however, about how the unique functional architecture of mosquito SGs is achieved. Using immunohistochemistry and confocal microscopy, we address two questions regarding SGs of the malaria vector Anopheles gambiae. How does the distinct cup-shaped morphology of SG secretory cells arise? And, how does the salivary duct, the structure through which saliva and parasites exit the glands, form? We demonstrate that SG cells begin as cuboidal-shaped cells surrounding a matrix-filled lumen that mature into cup-shaped cells through the formation and fusion of a large pre-apical compartment (PAC) to the apical surface. The secretory duct begins as buds of chitin at the apical surface of individual secretory cells. Further chitin deposition connects these chitin buds to form a contiguous duct that largely separates from the apical surface during PAC fusion.Michael B. WellsJordan VillamorDeborah J. AndrewNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael B. Wells Jordan Villamor Deborah J. Andrew Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
description	Abstract Mosquito-borne diseases cause one million deaths and hundreds of millions of human infections yearly. With all such diseases, the pathogen must traverse the mosquito salivary gland (SG) for transmission to a new host, making the SGs ideal targets for genetic strategies to block transmission. Prior studies have elucidated details of SG structure by light and electron microscopy and have deeply explored the salivary transcriptome and proteome. Very little is known, however, about how the unique functional architecture of mosquito SGs is achieved. Using immunohistochemistry and confocal microscopy, we address two questions regarding SGs of the malaria vector Anopheles gambiae. How does the distinct cup-shaped morphology of SG secretory cells arise? And, how does the salivary duct, the structure through which saliva and parasites exit the glands, form? We demonstrate that SG cells begin as cuboidal-shaped cells surrounding a matrix-filled lumen that mature into cup-shaped cells through the formation and fusion of a large pre-apical compartment (PAC) to the apical surface. The secretory duct begins as buds of chitin at the apical surface of individual secretory cells. Further chitin deposition connects these chitin buds to form a contiguous duct that largely separates from the apical surface during PAC fusion.
format	article
author	Michael B. Wells Jordan Villamor Deborah J. Andrew
author_facet	Michael B. Wells Jordan Villamor Deborah J. Andrew
author_sort	Michael B. Wells
title	Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
title_short	Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
title_full	Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
title_fullStr	Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
title_full_unstemmed	Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
title_sort	salivary gland maturation and duct formation in the african malaria mosquito anopheles gambiae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9768154b15284f5dbc3a5a974e185972
work_keys_str_mv	AT michaelbwells salivaryglandmaturationandductformationintheafricanmalariamosquitoanophelesgambiae AT jordanvillamor salivaryglandmaturationandductformationintheafricanmalariamosquitoanophelesgambiae AT deborahjandrew salivaryglandmaturationandductformationintheafricanmalariamosquitoanophelesgambiae
_version_	1718394880103809024

Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae

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