Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites

ABSTRACT Bisphenol A (BPA) accumulates in the maturing gut and liver in utero and is known to alter gut bacterial profiles in offspring. Gut bacterial dysbiosis may contribute to chronic colonic and systemic inflammation. We hypothesized that perinatal BPA exposure-induced intestinal (and liver) inf...

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Autores principales:	Lavanya Reddivari, D. N. Rao Veeramachaneni, William A. Walters, Catherine Lozupone, Jennifer Palmer, M. K. Kurundu Hewage, Rohil Bhatnagar, Amnon Amir, Mary J. Kennett, Rob Knight, Jairam K. P. Vanamala
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	amino acid metabolism endocrine disruptor gut permeability inflammation lipopolysaccharide microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/97686546786545bc96efdb86b1bc0bb3
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spelling	oai:doaj.org-article:97686546786545bc96efdb86b1bc0bb32021-12-02T18:39:46ZPerinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites10.1128/mSystems.00093-172379-5077https://doaj.org/article/97686546786545bc96efdb86b1bc0bb32017-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00093-17https://doaj.org/toc/2379-5077ABSTRACT Bisphenol A (BPA) accumulates in the maturing gut and liver in utero and is known to alter gut bacterial profiles in offspring. Gut bacterial dysbiosis may contribute to chronic colonic and systemic inflammation. We hypothesized that perinatal BPA exposure-induced intestinal (and liver) inflammation in offspring is due to alterations in the microbiome and colonic metabolome. The 16S rRNA amplicon sequencing analysis revealed differences in beta diversity with a significant reduction in the relative abundances of short-chain fatty acid (SCFA) producers such as Oscillospira and Ruminococcaceae due to BPA exposure. Furthermore, BPA exposure reduced fecal SCFA levels and increased systemic lipopolysaccharide (LPS) levels. BPA exposure-increased intestinal permeability was ameliorated by the addition of SCFA in vitro. Metabolic fingerprints revealed alterations in global metabolism and amino acid metabolism. Thus, our findings indicate that perinatal BPA exposure may cause gut bacterial dysbiosis and altered metabolite profiles, particularly SCFA profiles, leading to chronic colon and liver inflammation. IMPORTANCE Emerging evidence suggests that environmental toxicants may influence inflammation-promoted chronic disease susceptibility during early life. BPA, an environmental endocrine disruptor, can transfer across the placenta and accumulate in fetal gut and liver. However, underlying mechanisms for BPA-induced colonic and liver inflammation are not fully elucidated. In this report, we show how perinatal BPA exposure in rabbits alters gut microbiota and their metabolite profiles, which leads to colonic and liver inflammation as well as to increased gut permeability as measured by elevated serum lipopolysaccharide (LPS) levels in the offspring. Also, perinatal BPA exposure leads to reduced levels of gut bacterial diversity and bacterial metabolites (short-chain fatty acids [SCFA]) and elevated gut permeability—three common early biomarkers of inflammation-promoted chronic diseases. In addition, we showed that SCFA ameliorated BPA-induced intestinal permeability in vitro. Thus, our study results suggest that correcting environmental toxicant-induced bacterial dysbiosis early in life may reduce the risk of chronic diseases later in life.Lavanya ReddivariD. N. Rao VeeramachaneniWilliam A. WaltersCatherine LozuponeJennifer PalmerM. K. Kurundu HewageRohil BhatnagarAmnon AmirMary J. KennettRob KnightJairam K. P. VanamalaAmerican Society for Microbiologyarticleamino acid metabolismendocrine disruptorgut permeabilityinflammationlipopolysaccharidemicrobiomeMicrobiologyQR1-502ENmSystems, Vol 2, Iss 5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	amino acid metabolism endocrine disruptor gut permeability inflammation lipopolysaccharide microbiome Microbiology QR1-502
spellingShingle	amino acid metabolism endocrine disruptor gut permeability inflammation lipopolysaccharide microbiome Microbiology QR1-502 Lavanya Reddivari D. N. Rao Veeramachaneni William A. Walters Catherine Lozupone Jennifer Palmer M. K. Kurundu Hewage Rohil Bhatnagar Amnon Amir Mary J. Kennett Rob Knight Jairam K. P. Vanamala Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites
description	ABSTRACT Bisphenol A (BPA) accumulates in the maturing gut and liver in utero and is known to alter gut bacterial profiles in offspring. Gut bacterial dysbiosis may contribute to chronic colonic and systemic inflammation. We hypothesized that perinatal BPA exposure-induced intestinal (and liver) inflammation in offspring is due to alterations in the microbiome and colonic metabolome. The 16S rRNA amplicon sequencing analysis revealed differences in beta diversity with a significant reduction in the relative abundances of short-chain fatty acid (SCFA) producers such as Oscillospira and Ruminococcaceae due to BPA exposure. Furthermore, BPA exposure reduced fecal SCFA levels and increased systemic lipopolysaccharide (LPS) levels. BPA exposure-increased intestinal permeability was ameliorated by the addition of SCFA in vitro. Metabolic fingerprints revealed alterations in global metabolism and amino acid metabolism. Thus, our findings indicate that perinatal BPA exposure may cause gut bacterial dysbiosis and altered metabolite profiles, particularly SCFA profiles, leading to chronic colon and liver inflammation. IMPORTANCE Emerging evidence suggests that environmental toxicants may influence inflammation-promoted chronic disease susceptibility during early life. BPA, an environmental endocrine disruptor, can transfer across the placenta and accumulate in fetal gut and liver. However, underlying mechanisms for BPA-induced colonic and liver inflammation are not fully elucidated. In this report, we show how perinatal BPA exposure in rabbits alters gut microbiota and their metabolite profiles, which leads to colonic and liver inflammation as well as to increased gut permeability as measured by elevated serum lipopolysaccharide (LPS) levels in the offspring. Also, perinatal BPA exposure leads to reduced levels of gut bacterial diversity and bacterial metabolites (short-chain fatty acids [SCFA]) and elevated gut permeability—three common early biomarkers of inflammation-promoted chronic diseases. In addition, we showed that SCFA ameliorated BPA-induced intestinal permeability in vitro. Thus, our study results suggest that correcting environmental toxicant-induced bacterial dysbiosis early in life may reduce the risk of chronic diseases later in life.
format	article
author	Lavanya Reddivari D. N. Rao Veeramachaneni William A. Walters Catherine Lozupone Jennifer Palmer M. K. Kurundu Hewage Rohil Bhatnagar Amnon Amir Mary J. Kennett Rob Knight Jairam K. P. Vanamala
author_facet	Lavanya Reddivari D. N. Rao Veeramachaneni William A. Walters Catherine Lozupone Jennifer Palmer M. K. Kurundu Hewage Rohil Bhatnagar Amnon Amir Mary J. Kennett Rob Knight Jairam K. P. Vanamala
author_sort	Lavanya Reddivari
title	Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites
title_short	Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites
title_full	Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites
title_fullStr	Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites
title_full_unstemmed	Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites
title_sort	perinatal bisphenol a exposure induces chronic inflammation in rabbit offspring via modulation of gut bacteria and their metabolites
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/97686546786545bc96efdb86b1bc0bb3
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Perinatal Bisphenol A Exposure Induces Chronic Inflammation in Rabbit Offspring via Modulation of Gut Bacteria and Their Metabolites

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