Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila

Abstract The Drosophila model is used to investigate the effects of diet on physiology as well as the effects of genetic pathways, neural systems and environment on feeding behavior. We previously showed that Blue 1 works well as a dye tracer to track consumption of agar-based media in Drosophila in...

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Autores principales:	Brandon C. Shell, Yuan Luo, Scott Pletcher, Mike Grotewiel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97687cadaa5b494e946c31236dba4139
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spelling	oai:doaj.org-article:97687cadaa5b494e946c31236dba41392021-12-02T17:13:18ZExpansion and application of dye tracers for measuring solid food intake and food preference in Drosophila10.1038/s41598-021-99483-72045-2322https://doaj.org/article/97687cadaa5b494e946c31236dba41392021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99483-7https://doaj.org/toc/2045-2322Abstract The Drosophila model is used to investigate the effects of diet on physiology as well as the effects of genetic pathways, neural systems and environment on feeding behavior. We previously showed that Blue 1 works well as a dye tracer to track consumption of agar-based media in Drosophila in a method called Con-Ex. Here, we describe Orange 4 as a novel dye for use in Con-Ex studies that expands the utility of this method. Con-Ex experiments using Orange 4 detect the predicted effects of starvation, mating status, strain, and sex on feeding behavior in flies. Orange 4 is consumed and excreted into vials linearly with time in Con-Ex experiments, the number of replicates required to detect differences between groups when using Orange 4 is comparable to that for Blue 1, and excretion of the dye reflects the volume of consumed dye. In food preference studies using Orange 4 and Blue 1 as a dye pair, flies decreased their intake of food laced with the aversive tastants caffeine and NaCl as determined using Con-Ex or a more recently described modification called EX-Q. Our results indicate that Orange 4 is suitable for Con-Ex experiments, has comparable utility to Blue 1 in Con-Ex studies, and can be paired with Blue 1 to assess food preference via both Con-Ex and EX-Q.Brandon C. ShellYuan LuoScott PletcherMike GrotewielNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brandon C. Shell Yuan Luo Scott Pletcher Mike Grotewiel Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila
description	Abstract The Drosophila model is used to investigate the effects of diet on physiology as well as the effects of genetic pathways, neural systems and environment on feeding behavior. We previously showed that Blue 1 works well as a dye tracer to track consumption of agar-based media in Drosophila in a method called Con-Ex. Here, we describe Orange 4 as a novel dye for use in Con-Ex studies that expands the utility of this method. Con-Ex experiments using Orange 4 detect the predicted effects of starvation, mating status, strain, and sex on feeding behavior in flies. Orange 4 is consumed and excreted into vials linearly with time in Con-Ex experiments, the number of replicates required to detect differences between groups when using Orange 4 is comparable to that for Blue 1, and excretion of the dye reflects the volume of consumed dye. In food preference studies using Orange 4 and Blue 1 as a dye pair, flies decreased their intake of food laced with the aversive tastants caffeine and NaCl as determined using Con-Ex or a more recently described modification called EX-Q. Our results indicate that Orange 4 is suitable for Con-Ex experiments, has comparable utility to Blue 1 in Con-Ex studies, and can be paired with Blue 1 to assess food preference via both Con-Ex and EX-Q.
format	article
author	Brandon C. Shell Yuan Luo Scott Pletcher Mike Grotewiel
author_facet	Brandon C. Shell Yuan Luo Scott Pletcher Mike Grotewiel
author_sort	Brandon C. Shell
title	Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila
title_short	Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila
title_full	Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila
title_fullStr	Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila
title_full_unstemmed	Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila
title_sort	expansion and application of dye tracers for measuring solid food intake and food preference in drosophila
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/97687cadaa5b494e946c31236dba4139
work_keys_str_mv	AT brandoncshell expansionandapplicationofdyetracersformeasuringsolidfoodintakeandfoodpreferenceindrosophila AT yuanluo expansionandapplicationofdyetracersformeasuringsolidfoodintakeandfoodpreferenceindrosophila AT scottpletcher expansionandapplicationofdyetracersformeasuringsolidfoodintakeandfoodpreferenceindrosophila AT mikegrotewiel expansionandapplicationofdyetracersformeasuringsolidfoodintakeandfoodpreferenceindrosophila
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Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila

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