A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report

mRNA and Adenovirus vaccines for COVID-19 are used to induce humoral and cell-mediated immunity, with the aim to generate both SARS-CoV-2 B and T memory cells. In present study, we described a simple assay to detect and quantify Spike-specific CD4<sup>+</sup> and CD8<sup>+</sup&...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mojtaba Shekarkar Azgomi, Marco Pio La Manna, Giusto Davide Badami, Paolo Ragonese, Antonino Trizzino, Francesco Dieli, Nadia Caccamo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Pfizer/BioNTech memory T cell CD4<sup>+</sup> CD8<sup>+</sup> SARS-CoV-2 cytokines Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/976951fe4c8b4ac8b4fd040f481cf697
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:976951fe4c8b4ac8b4fd040f481cf697
record_format	dspace
spelling	oai:doaj.org-article:976951fe4c8b4ac8b4fd040f481cf6972021-11-25T16:49:11ZA Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report10.3390/biomedicines91115762227-9059https://doaj.org/article/976951fe4c8b4ac8b4fd040f481cf6972021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1576https://doaj.org/toc/2227-9059mRNA and Adenovirus vaccines for COVID-19 are used to induce humoral and cell-mediated immunity, with the aim to generate both SARS-CoV-2 B and T memory cells. In present study, we described a simple assay to detect and quantify Spike-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cell responses induced by vaccination in healthy donors and in subjects with B cell compart impairment, in which antibody response is absent due to primary immunodeficiencies or CD20 depleting therapy. We detect and quantified memory T cell immune responses against SARS-CoV-2 evocated by vaccination in both groups, irrespective to the humoral response. Furthermore, we identified TNF-α as the main cytokine produced by T memory cells, after antigen-specific stimulation in vitro, that could be considered, other than IFN-γ, an additional biomarker of induction of T memory cells upon vaccination. Further studies on the vaccine-induced T cell responses could be crucial, not only in healthy people but also in immunocompromised subjects, where antigen specific T cells responses play a protective role against SARS-CoV-2.Mojtaba Shekarkar AzgomiMarco Pio La MannaGiusto Davide BadamiPaolo RagoneseAntonino TrizzinoFrancesco DieliNadia CaccamoMDPI AGarticlePfizer/BioNTechmemory T cellCD4<sup>+</sup>CD8<sup>+</sup>SARS-CoV-2cytokinesBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1576, p 1576 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pfizer/BioNTech memory T cell CD4<sup>+</sup> CD8<sup>+</sup> SARS-CoV-2 cytokines Biology (General) QH301-705.5
spellingShingle	Pfizer/BioNTech memory T cell CD4<sup>+</sup> CD8<sup>+</sup> SARS-CoV-2 cytokines Biology (General) QH301-705.5 Mojtaba Shekarkar Azgomi Marco Pio La Manna Giusto Davide Badami Paolo Ragonese Antonino Trizzino Francesco Dieli Nadia Caccamo A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report
description	mRNA and Adenovirus vaccines for COVID-19 are used to induce humoral and cell-mediated immunity, with the aim to generate both SARS-CoV-2 B and T memory cells. In present study, we described a simple assay to detect and quantify Spike-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cell responses induced by vaccination in healthy donors and in subjects with B cell compart impairment, in which antibody response is absent due to primary immunodeficiencies or CD20 depleting therapy. We detect and quantified memory T cell immune responses against SARS-CoV-2 evocated by vaccination in both groups, irrespective to the humoral response. Furthermore, we identified TNF-α as the main cytokine produced by T memory cells, after antigen-specific stimulation in vitro, that could be considered, other than IFN-γ, an additional biomarker of induction of T memory cells upon vaccination. Further studies on the vaccine-induced T cell responses could be crucial, not only in healthy people but also in immunocompromised subjects, where antigen specific T cells responses play a protective role against SARS-CoV-2.
format	article
author	Mojtaba Shekarkar Azgomi Marco Pio La Manna Giusto Davide Badami Paolo Ragonese Antonino Trizzino Francesco Dieli Nadia Caccamo
author_facet	Mojtaba Shekarkar Azgomi Marco Pio La Manna Giusto Davide Badami Paolo Ragonese Antonino Trizzino Francesco Dieli Nadia Caccamo
author_sort	Mojtaba Shekarkar Azgomi
title	A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report
title_short	A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report
title_full	A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report
title_fullStr	A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report
title_full_unstemmed	A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report
title_sort	rapid and simple multiparameter assay to quantify spike-specific cd4 and cd8 t cells after sars-cov-2 vaccination: a preliminary report
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/976951fe4c8b4ac8b4fd040f481cf697
work_keys_str_mv	AT mojtabashekarkarazgomi arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT marcopiolamanna arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT giustodavidebadami arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT paoloragonese arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT antoninotrizzino arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT francescodieli arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT nadiacaccamo arapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT mojtabashekarkarazgomi rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT marcopiolamanna rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT giustodavidebadami rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT paoloragonese rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT antoninotrizzino rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT francescodieli rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport AT nadiacaccamo rapidandsimplemultiparameterassaytoquantifyspikespecificcd4andcd8tcellsaftersarscov2vaccinationapreliminaryreport
_version_	1718412971138351104

A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report

Ejemplares similares