Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites

For more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s...

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Autores principales:	Cristina Isabel Fernández-Otero, Ana María Ramos-Cabrer, Julio Enrique López-Díaz, Santiago Pereira-Lorenzo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	simple sequence repeats (SSRs) variability morphology local cultivars germplasm bank Agriculture S
Acceso en línea:	https://doaj.org/article/976d6be3c24241a587ded17d12e45000
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spelling	oai:doaj.org-article:976d6be3c24241a587ded17d12e450002021-11-25T16:09:53ZEvaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites10.3390/agronomy111122752073-4395https://doaj.org/article/976d6be3c24241a587ded17d12e450002021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2275https://doaj.org/toc/2073-4395For more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s germplasm bank has 57 populations of red clover (<i>Trifolium pratense</i> Lam.), one of the most cultivated forage legumes in the world. The goal of the present study was to evaluate the diversity among cultivars and natural clover populations at morphological and molecular level. Twelve polymorphic SSR loci revealed 241 microsatellite alleles with an average of 20.08 alleles per locus. Two main groups were detected by the Structure software, one of them including local populations and the second clustering cultivars and related populations. Intra-specific variability was found among cultivars and natural populations. A moderate genetic differentiation of Spanish red clover cultivars was observed (Fst = 0.08) between the two main clusters. Finally, a certain relationship between phenotypic and genotypic variation was detected.Cristina Isabel Fernández-OteroAna María Ramos-CabrerJulio Enrique López-DíazSantiago Pereira-LorenzoMDPI AGarticlesimple sequence repeats (SSRs)variabilitymorphologylocal cultivarsgermplasm bankAgricultureSENAgronomy, Vol 11, Iss 2275, p 2275 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	simple sequence repeats (SSRs) variability morphology local cultivars germplasm bank Agriculture S
spellingShingle	simple sequence repeats (SSRs) variability morphology local cultivars germplasm bank Agriculture S Cristina Isabel Fernández-Otero Ana María Ramos-Cabrer Julio Enrique López-Díaz Santiago Pereira-Lorenzo Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
description	For more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s germplasm bank has 57 populations of red clover (<i>Trifolium pratense</i> Lam.), one of the most cultivated forage legumes in the world. The goal of the present study was to evaluate the diversity among cultivars and natural clover populations at morphological and molecular level. Twelve polymorphic SSR loci revealed 241 microsatellite alleles with an average of 20.08 alleles per locus. Two main groups were detected by the Structure software, one of them including local populations and the second clustering cultivars and related populations. Intra-specific variability was found among cultivars and natural populations. A moderate genetic differentiation of Spanish red clover cultivars was observed (Fst = 0.08) between the two main clusters. Finally, a certain relationship between phenotypic and genotypic variation was detected.
format	article
author	Cristina Isabel Fernández-Otero Ana María Ramos-Cabrer Julio Enrique López-Díaz Santiago Pereira-Lorenzo
author_facet	Cristina Isabel Fernández-Otero Ana María Ramos-Cabrer Julio Enrique López-Díaz Santiago Pereira-Lorenzo
author_sort	Cristina Isabel Fernández-Otero
title	Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
title_short	Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
title_full	Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
title_fullStr	Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
title_full_unstemmed	Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
title_sort	evaluating the diversity of ecotypes of red clover (<i>trifolium pratense</i> l.) from northwestern spain by phenotypic traits and microsatellites
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/976d6be3c24241a587ded17d12e45000
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Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites

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