Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory

Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more li...

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Autores principales:	Kristen Bowey-Dellinger, Luke Dixon, Kristin Ackerman, Cynthia Vigueira, Yewseok K. Suh, Todd Lyda, Kelli Sapp, Michael Grider, Dinene Crater, Travis Russell, Michael Elias, V. McNeil Coffield, Verónica A. Segarra
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/977be318791e4c7f97f1ae08fc13c5b3
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spelling	oai:doaj.org-article:977be318791e4c7f97f1ae08fc13c5b32021-11-15T15:04:06ZIntroducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory10.1128/jmbe.v18i2.12641935-78851935-7877https://doaj.org/article/977be318791e4c7f97f1ae08fc13c5b32017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i2.1264https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, culture, and manipulate given the equipment, tools, and environment of most undergraduate biology laboratories. In contrast, the utilization of mammalian cells requires a dedicated biological safety cabinet and rigorous antiseptic techniques. For this reason, we have devised a laboratory module and method herein that familiarizes students with common cell culture procedures, without the use of a sterile hood or large cell culture facility. Students design and perform a time-efficient inquiry-based cell viability experiment using HeLa cells and tools that are readily available in an undergraduate biology laboratory. Students will become familiar with common techniques such as trypsinizing cells, cell counting with a hemocytometer, performing serial dilutions, and determining cell viability using trypan blue dye. Additionally, students will work with graphing software to analyze their data and think critically about the mechanism of death on a cellular level. Two different adaptations of this inquiry-based lab are presented—one for non-biology majors and one for biology majors. Overall, these laboratories aim to expose students to mammalian cell culture and basic techniques and help them to conceptualize their application in scientific research.Kristen Bowey-DellingerLuke DixonKristin AckermanCynthia VigueiraYewseok K. SuhTodd LydaKelli SappMichael GriderDinene CraterTravis RussellMichael EliasV. McNeil CoffieldVerónica A. SegarraAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Kristen Bowey-Dellinger Luke Dixon Kristin Ackerman Cynthia Vigueira Yewseok K. Suh Todd Lyda Kelli Sapp Michael Grider Dinene Crater Travis Russell Michael Elias V. McNeil Coffield Verónica A. Segarra Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
description	Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, culture, and manipulate given the equipment, tools, and environment of most undergraduate biology laboratories. In contrast, the utilization of mammalian cells requires a dedicated biological safety cabinet and rigorous antiseptic techniques. For this reason, we have devised a laboratory module and method herein that familiarizes students with common cell culture procedures, without the use of a sterile hood or large cell culture facility. Students design and perform a time-efficient inquiry-based cell viability experiment using HeLa cells and tools that are readily available in an undergraduate biology laboratory. Students will become familiar with common techniques such as trypsinizing cells, cell counting with a hemocytometer, performing serial dilutions, and determining cell viability using trypan blue dye. Additionally, students will work with graphing software to analyze their data and think critically about the mechanism of death on a cellular level. Two different adaptations of this inquiry-based lab are presented—one for non-biology majors and one for biology majors. Overall, these laboratories aim to expose students to mammalian cell culture and basic techniques and help them to conceptualize their application in scientific research.
format	article
author	Kristen Bowey-Dellinger Luke Dixon Kristin Ackerman Cynthia Vigueira Yewseok K. Suh Todd Lyda Kelli Sapp Michael Grider Dinene Crater Travis Russell Michael Elias V. McNeil Coffield Verónica A. Segarra
author_facet	Kristen Bowey-Dellinger Luke Dixon Kristin Ackerman Cynthia Vigueira Yewseok K. Suh Todd Lyda Kelli Sapp Michael Grider Dinene Crater Travis Russell Michael Elias V. McNeil Coffield Verónica A. Segarra
author_sort	Kristen Bowey-Dellinger
title	Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
title_short	Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
title_full	Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
title_fullStr	Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
title_full_unstemmed	Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
title_sort	introducing mammalian cell culture and cell viability techniques in the undergraduate biology laboratory
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/977be318791e4c7f97f1ae08fc13c5b3
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Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory

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