Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma

BackgroundHepatocellular carcinoma (HCC) is an extremely malignant cancer with poor survival. H2AFY gene encodes for a variant of H2A histone, and it has been found to be dysregulated in various tumors. However, the clinical value, biological functions and correlations with immune infiltration of H2...

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Autores principales:	Yongbiao Huang, Shanshan Huang, Li Ma, Yali Wang, Xi Wang, Lingyan Xiao, Wan Qin, Long Li, Xianglin Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hepatocellular carcinoma H2AFY prognosis immune infiltration biomarker Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/977d0f56099c4dc1b0fb0e63b6cedd2a
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spelling	oai:doaj.org-article:977d0f56099c4dc1b0fb0e63b6cedd2a2021-11-30T17:17:57ZExploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma1664-322410.3389/fimmu.2021.723293https://doaj.org/article/977d0f56099c4dc1b0fb0e63b6cedd2a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.723293/fullhttps://doaj.org/toc/1664-3224BackgroundHepatocellular carcinoma (HCC) is an extremely malignant cancer with poor survival. H2AFY gene encodes for a variant of H2A histone, and it has been found to be dysregulated in various tumors. However, the clinical value, biological functions and correlations with immune infiltration of H2AFY in HCC remain unclear.MethodsWe analyzed the expression and clinical significance of H2AFY in HCC using multiple databases, including Oncomine, HCCDB, TCGA, ICGC, and so on. The genetic alterations of H2AFY were analyzed by cBioPortal and COSMIC databases. Co-expression networks of H2AFY and its regulators were investigated by LinkedOmics. The correlations between H2AFY and tumor immune infiltration were explored using TIMER, TISIDB databases, and CIBERSORT method. Finally, H2AFY was knocked down with shRNA lentiviruses in HCC cell lines for functional assays in vitro.ResultsH2AFY expression was upregulated in the HCC tissues and cells. Kaplan–Meier and Cox regression analyses revealed that high H2AFY expression was an independent prognostic factor for poor survival in HCC patients. Functional network analysis indicated that H2AFY and its co-expressed genes regulates cell cycle, mitosis, spliceosome and chromatin assembly through pathways involving many cancer-related kinases and E2F family. Furthermore, we observed significant correlations between H2AFY expression and immune infiltration in HCC. H2AFY knockdown suppressed the cell proliferation and migration, promoted cycle arrest, and apoptosis of HCC cells in vitro.ConclusionOur study revealed that H2AFY is a potential biomarker for unfavorable prognosis and correlates with immune infiltration in HCC.Yongbiao HuangShanshan HuangLi MaYali WangXi WangLingyan XiaoWan QinLong LiXianglin YuanFrontiers Media S.A.articlehepatocellular carcinomaH2AFYprognosisimmune infiltrationbiomarkerImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatocellular carcinoma H2AFY prognosis immune infiltration biomarker Immunologic diseases. Allergy RC581-607
spellingShingle	hepatocellular carcinoma H2AFY prognosis immune infiltration biomarker Immunologic diseases. Allergy RC581-607 Yongbiao Huang Shanshan Huang Li Ma Yali Wang Xi Wang Lingyan Xiao Wan Qin Long Li Xianglin Yuan Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
description	BackgroundHepatocellular carcinoma (HCC) is an extremely malignant cancer with poor survival. H2AFY gene encodes for a variant of H2A histone, and it has been found to be dysregulated in various tumors. However, the clinical value, biological functions and correlations with immune infiltration of H2AFY in HCC remain unclear.MethodsWe analyzed the expression and clinical significance of H2AFY in HCC using multiple databases, including Oncomine, HCCDB, TCGA, ICGC, and so on. The genetic alterations of H2AFY were analyzed by cBioPortal and COSMIC databases. Co-expression networks of H2AFY and its regulators were investigated by LinkedOmics. The correlations between H2AFY and tumor immune infiltration were explored using TIMER, TISIDB databases, and CIBERSORT method. Finally, H2AFY was knocked down with shRNA lentiviruses in HCC cell lines for functional assays in vitro.ResultsH2AFY expression was upregulated in the HCC tissues and cells. Kaplan–Meier and Cox regression analyses revealed that high H2AFY expression was an independent prognostic factor for poor survival in HCC patients. Functional network analysis indicated that H2AFY and its co-expressed genes regulates cell cycle, mitosis, spliceosome and chromatin assembly through pathways involving many cancer-related kinases and E2F family. Furthermore, we observed significant correlations between H2AFY expression and immune infiltration in HCC. H2AFY knockdown suppressed the cell proliferation and migration, promoted cycle arrest, and apoptosis of HCC cells in vitro.ConclusionOur study revealed that H2AFY is a potential biomarker for unfavorable prognosis and correlates with immune infiltration in HCC.
format	article
author	Yongbiao Huang Shanshan Huang Li Ma Yali Wang Xi Wang Lingyan Xiao Wan Qin Long Li Xianglin Yuan
author_facet	Yongbiao Huang Shanshan Huang Li Ma Yali Wang Xi Wang Lingyan Xiao Wan Qin Long Li Xianglin Yuan
author_sort	Yongbiao Huang
title	Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
title_short	Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
title_full	Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
title_fullStr	Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
title_full_unstemmed	Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
title_sort	exploring the prognostic value, immune implication and biological function of h2afy gene in hepatocellular carcinoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/977d0f56099c4dc1b0fb0e63b6cedd2a
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Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma

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