Effects of the protein corona on liposome–liposome and liposome–cell interactions

Effects of the protein corona on liposome–liposome and liposome–cell interactions

Claudia Corbo,1,2 Roberto Molinaro,1 Francesca Taraballi,1 Naama E Toledano Furman,1 Michael B Sherman,3 Alessandro Parodi,1 Francesco Salvatore,2,4 Ennio Tasciotti1,5 1Center for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, USA; 2CEINGE-Biotecnologie Avanzate s.c.a r.l.,...

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Autores principales: Corbo C, Molinaro R, Taraballi F, Toledano Furman NE, Sherman MB, Parodi A, Salvatore F, Tasciotti E
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
liposomes
protein corona
macrophages
cancer cells
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/977fbe2aeb31494cb513231c7bf775c7
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spelling oai:doaj.org-article:977fbe2aeb31494cb513231c7bf775c72021-12-02T02:43:42ZEffects of the protein corona on liposome–liposome and liposome–cell interactions1178-2013https://doaj.org/article/977fbe2aeb31494cb513231c7bf775c72016-07-01T00:00:00Zhttps://www.dovepress.com/effects-of-the-protein-corona-on-liposomendashliposome-and-liposomenda-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Claudia Corbo,1,2 Roberto Molinaro,1 Francesca Taraballi,1 Naama E Toledano Furman,1 Michael B Sherman,3 Alessandro Parodi,1 Francesco Salvatore,2,4 Ennio Tasciotti1,5 1Center for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, USA; 2CEINGE-Biotecnologie Avanzate s.c.a r.l., Naples, Italy; 3Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA; 4Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Naples, Italy; 5Department of Orthopedics, Houston Methodist Hospital, Houston, TX, USA Abstract: A thorough understanding of interactions occurring at the interface between nanocarriers and biological systems is crucial to predict and interpret their biodistribution, targeting, and efficacy, and thus design more effective drug delivery systems. Upon intravenous injection, nanoparticles are coated by a protein corona (PC). This confers a new biological identity on the particles that largely determines their biological fate. Liposomes have great pharmaceutical versatility, so, as proof of concept, their PC has recently been implicated in the mechanism and efficiency of their internalization into the cell. In an attempt to better understand the interactions between nanocarriers and biological systems, we analyzed the plasma proteins adsorbed on the surface of multicomponent liposomes. Specifically, we analyzed the physical properties and ultrastructure of liposome/PC complexes and the aggregation process that occurs when liposomes are dispersed in plasma. The results of combined confocal microscopy and flow cytometry experiments demonstrated that the PC favors liposome internalization by both macrophages and tumor cells. This work provides insights into the effects of the PC on liposomes’ physical properties and, consequently, liposome–liposome and liposome–cell interactions. Keywords: liposomes, protein corona, macrophages, cancer cellsCorbo CMolinaro RTaraballi FToledano Furman NESherman MBParodi ASalvatore FTasciotti EDove Medical Pressarticleliposomesprotein coronamacrophagescancer cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3049-3063 (2016)
institution DOAJ
collection DOAJ
language EN
topic liposomes
protein corona
macrophages
cancer cells
Medicine (General)
R5-920
spellingShingle liposomes
protein corona
macrophages
cancer cells
Medicine (General)
R5-920
Corbo C
Molinaro R
Taraballi F
Toledano Furman NE
Sherman MB
Parodi A
Salvatore F
Tasciotti E
Effects of the protein corona on liposome–liposome and liposome–cell interactions
description Claudia Corbo,1,2 Roberto Molinaro,1 Francesca Taraballi,1 Naama E Toledano Furman,1 Michael B Sherman,3 Alessandro Parodi,1 Francesco Salvatore,2,4 Ennio Tasciotti1,5 1Center for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, USA; 2CEINGE-Biotecnologie Avanzate s.c.a r.l., Naples, Italy; 3Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA; 4Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Naples, Italy; 5Department of Orthopedics, Houston Methodist Hospital, Houston, TX, USA Abstract: A thorough understanding of interactions occurring at the interface between nanocarriers and biological systems is crucial to predict and interpret their biodistribution, targeting, and efficacy, and thus design more effective drug delivery systems. Upon intravenous injection, nanoparticles are coated by a protein corona (PC). This confers a new biological identity on the particles that largely determines their biological fate. Liposomes have great pharmaceutical versatility, so, as proof of concept, their PC has recently been implicated in the mechanism and efficiency of their internalization into the cell. In an attempt to better understand the interactions between nanocarriers and biological systems, we analyzed the plasma proteins adsorbed on the surface of multicomponent liposomes. Specifically, we analyzed the physical properties and ultrastructure of liposome/PC complexes and the aggregation process that occurs when liposomes are dispersed in plasma. The results of combined confocal microscopy and flow cytometry experiments demonstrated that the PC favors liposome internalization by both macrophages and tumor cells. This work provides insights into the effects of the PC on liposomes’ physical properties and, consequently, liposome–liposome and liposome–cell interactions. Keywords: liposomes, protein corona, macrophages, cancer cells
format article
author Corbo C
Molinaro R
Taraballi F
Toledano Furman NE
Sherman MB
Parodi A
Salvatore F
Tasciotti E
author_facet Corbo C
Molinaro R
Taraballi F
Toledano Furman NE
Sherman MB
Parodi A
Salvatore F
Tasciotti E
author_sort Corbo C
title Effects of the protein corona on liposome–liposome and liposome–cell interactions
title_short Effects of the protein corona on liposome–liposome and liposome–cell interactions
title_full Effects of the protein corona on liposome–liposome and liposome–cell interactions
title_fullStr Effects of the protein corona on liposome–liposome and liposome–cell interactions
title_full_unstemmed Effects of the protein corona on liposome–liposome and liposome–cell interactions
title_sort effects of the protein corona on liposome–liposome and liposome–cell interactions
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/977fbe2aeb31494cb513231c7bf775c7
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