Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax)

Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax)

ABSTRACT High-throughput 16S rRNA gene amplicon sequencing is an essential method for studying the diversity and dynamics of microbial communities. However, this method is presently hampered by the lack of high-identity reference sequences for many environmental microbes in the public 16S rRNA gene...

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Detalles Bibliográficos
Autores principales: Morten Simonsen Dueholm, Kasper Skytte Andersen, Simon Jon McIlroy, Jannie Munk Kristensen, Erika Yashiro, Søren Michael Karst, Mads Albertsen, Per Halkjær Nielsen
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2020
Materias:
16S RNA
gene sequencing
microbial communities
microbial ecology
taxonomy
wastewater treatment
Microbiology
QR1-502
Acceso en línea:https://doaj.org/article/97839cfdc7064442bf889f2a3f10e3d7
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