Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity

There is growing evidence that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) regulates inflammatory responses. Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized with synovitis and joint destruction. Therefore, this study was planned to investigate the rela...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	In Seol Yoo, Yu-Ran Lee, Seong Wook Kang, Jinhyun Kim, Hee-Kyoung Joo, Su-Jin Yoo, Chan Keol Park, Ha-Reum Lee, Ji Ah Park, Byeong-Hwa Jeon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apurinic/apyrimidinic endonuclease 1/redox factor-1 rheumatoid arthritis DAS28 ELISA Medicine R
Acceso en línea:	https://doaj.org/article/9787769bfdcb4012916a05dfc53e461c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9787769bfdcb4012916a05dfc53e461c
record_format	dspace
spelling	oai:doaj.org-article:9787769bfdcb4012916a05dfc53e461c2021-11-25T18:01:46ZElevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity10.3390/jcm102253242077-0383https://doaj.org/article/9787769bfdcb4012916a05dfc53e461c2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/22/5324https://doaj.org/toc/2077-0383There is growing evidence that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) regulates inflammatory responses. Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized with synovitis and joint destruction. Therefore, this study was planned to investigate the relationship between APE1/Ref-1 and RA. Serum and synovial fluid (SF) were collected from 46 patients with RA, 45 patients with osteoarthritis (OA), and 30 healthy control (HC) patients. The concentration of APE1/Ref-1 in serum or SF was measured using the sandwich enzyme-linked immunosorbent assay (ELISA). The disease activity in RA patients was measured using the 28-joint disease activity score (DAS28). The serum APE1/Ref-1 levels in RA patients were significantly increased compared to HC and OA patients (0.44 ± 0.39 ng/mL for RA group vs. 0.19 ± 0.14 ng/mL for HC group, <i>p</i> < 0.05 and vs. 0.19 ± 0.11 ng/mL for OA group, <i>p</i> < 0.05). Likewise, the APE1/Ref-1 levels of SF in RA patients were also significantly increased compared to OA patients (0.68 ± 0.30 ng/mL for RA group vs. 0.31 ± 0.12 ng/mL for OA group, <i>p</i> < 0.001). The APE1/Ref-1 concentration in SF of RA patients was positively correlated with DAS28. Thus, APE1/Ref-1 may reflect the joint inflammation and be associated with disease activity in RA.In Seol YooYu-Ran LeeSeong Wook KangJinhyun KimHee-Kyoung JooSu-Jin YooChan Keol ParkHa-Reum LeeJi Ah ParkByeong-Hwa JeonMDPI AGarticleapurinic/apyrimidinic endonuclease 1/redox factor-1rheumatoid arthritisDAS28ELISAMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5324, p 5324 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apurinic/apyrimidinic endonuclease 1/redox factor-1 rheumatoid arthritis DAS28 ELISA Medicine R
spellingShingle	apurinic/apyrimidinic endonuclease 1/redox factor-1 rheumatoid arthritis DAS28 ELISA Medicine R In Seol Yoo Yu-Ran Lee Seong Wook Kang Jinhyun Kim Hee-Kyoung Joo Su-Jin Yoo Chan Keol Park Ha-Reum Lee Ji Ah Park Byeong-Hwa Jeon Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity
description	There is growing evidence that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) regulates inflammatory responses. Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized with synovitis and joint destruction. Therefore, this study was planned to investigate the relationship between APE1/Ref-1 and RA. Serum and synovial fluid (SF) were collected from 46 patients with RA, 45 patients with osteoarthritis (OA), and 30 healthy control (HC) patients. The concentration of APE1/Ref-1 in serum or SF was measured using the sandwich enzyme-linked immunosorbent assay (ELISA). The disease activity in RA patients was measured using the 28-joint disease activity score (DAS28). The serum APE1/Ref-1 levels in RA patients were significantly increased compared to HC and OA patients (0.44 ± 0.39 ng/mL for RA group vs. 0.19 ± 0.14 ng/mL for HC group, <i>p</i> < 0.05 and vs. 0.19 ± 0.11 ng/mL for OA group, <i>p</i> < 0.05). Likewise, the APE1/Ref-1 levels of SF in RA patients were also significantly increased compared to OA patients (0.68 ± 0.30 ng/mL for RA group vs. 0.31 ± 0.12 ng/mL for OA group, <i>p</i> < 0.001). The APE1/Ref-1 concentration in SF of RA patients was positively correlated with DAS28. Thus, APE1/Ref-1 may reflect the joint inflammation and be associated with disease activity in RA.
format	article
author	In Seol Yoo Yu-Ran Lee Seong Wook Kang Jinhyun Kim Hee-Kyoung Joo Su-Jin Yoo Chan Keol Park Ha-Reum Lee Ji Ah Park Byeong-Hwa Jeon
author_facet	In Seol Yoo Yu-Ran Lee Seong Wook Kang Jinhyun Kim Hee-Kyoung Joo Su-Jin Yoo Chan Keol Park Ha-Reum Lee Ji Ah Park Byeong-Hwa Jeon
author_sort	In Seol Yoo
title	Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity
title_short	Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity
title_full	Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity
title_fullStr	Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity
title_full_unstemmed	Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity
title_sort	elevated ape1/ref-1 levels of synovial fluids in patients with rheumatoid arthritis: reflection of disease activity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9787769bfdcb4012916a05dfc53e461c
work_keys_str_mv	AT inseolyoo elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT yuranlee elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT seongwookkang elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT jinhyunkim elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT heekyoungjoo elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT sujinyoo elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT chankeolpark elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT hareumlee elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT jiahpark elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity AT byeonghwajeon elevatedape1ref1levelsofsynovialfluidsinpatientswithrheumatoidarthritisreflectionofdiseaseactivity
_version_	1718411760900243456

Elevated APE1/Ref-1 Levels of Synovial Fluids in Patients with Rheumatoid Arthritis: Reflection of Disease Activity

Ejemplares similares