In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

The amount of genetic material that can be packaged in AAV vectors used for genome editing is limited. Here the authors show that the smallest known Cas9 orthologue, cjCas9, can be packaged in a single AAV vector along with sgRNA and a marker gene, and demonstrate efficient gene editing in mice.

Guardado en:

Detalles Bibliográficos
Autores principales:	Eunji Kim, Taeyoung Koo, Sung Wook Park, Daesik Kim, Kyoungmi Kim, Hee-Yeon Cho, Dong Woo Song, Kyu Jun Lee, Min Hee Jung, Seokjoong Kim, Jin Hyoung Kim, Jeong Hun Kim, Jin-Soo Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/979620e5b6d94aa09d84d0a42e5ed2a6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:979620e5b6d94aa09d84d0a42e5ed2a6
record_format	dspace
spelling	oai:doaj.org-article:979620e5b6d94aa09d84d0a42e5ed2a62021-12-02T15:38:50ZIn vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni10.1038/ncomms145002041-1723https://doaj.org/article/979620e5b6d94aa09d84d0a42e5ed2a62017-02-01T00:00:00Zhttps://doi.org/10.1038/ncomms14500https://doaj.org/toc/2041-1723The amount of genetic material that can be packaged in AAV vectors used for genome editing is limited. Here the authors show that the smallest known Cas9 orthologue, cjCas9, can be packaged in a single AAV vector along with sgRNA and a marker gene, and demonstrate efficient gene editing in mice.Eunji KimTaeyoung KooSung Wook ParkDaesik KimKyoungmi KimHee-Yeon ChoDong Woo SongKyu Jun LeeMin Hee JungSeokjoong KimJin Hyoung KimJeong Hun KimJin-Soo KimNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Eunji Kim Taeyoung Koo Sung Wook Park Daesik Kim Kyoungmi Kim Hee-Yeon Cho Dong Woo Song Kyu Jun Lee Min Hee Jung Seokjoong Kim Jin Hyoung Kim Jeong Hun Kim Jin-Soo Kim In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
description	The amount of genetic material that can be packaged in AAV vectors used for genome editing is limited. Here the authors show that the smallest known Cas9 orthologue, cjCas9, can be packaged in a single AAV vector along with sgRNA and a marker gene, and demonstrate efficient gene editing in mice.
format	article
author	Eunji Kim Taeyoung Koo Sung Wook Park Daesik Kim Kyoungmi Kim Hee-Yeon Cho Dong Woo Song Kyu Jun Lee Min Hee Jung Seokjoong Kim Jin Hyoung Kim Jeong Hun Kim Jin-Soo Kim
author_facet	Eunji Kim Taeyoung Koo Sung Wook Park Daesik Kim Kyoungmi Kim Hee-Yeon Cho Dong Woo Song Kyu Jun Lee Min Hee Jung Seokjoong Kim Jin Hyoung Kim Jeong Hun Kim Jin-Soo Kim
author_sort	Eunji Kim
title	In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
title_short	In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
title_full	In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
title_fullStr	In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
title_full_unstemmed	In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
title_sort	in vivo genome editing with a small cas9 orthologue derived from campylobacter jejuni
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/979620e5b6d94aa09d84d0a42e5ed2a6
work_keys_str_mv	AT eunjikim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT taeyoungkoo invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT sungwookpark invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT daesikkim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT kyoungmikim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT heeyeoncho invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT dongwoosong invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT kyujunlee invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT minheejung invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT seokjoongkim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT jinhyoungkim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT jeonghunkim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni AT jinsookim invivogenomeeditingwithasmallcas9orthologuederivedfromcampylobacterjejuni
_version_	1718386062368178176

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

Ejemplares similares