System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.

Inducible transgene expression provides a useful tool to analyze gene function. The moss Physcomitrellapatens is a model basal land plant with well-developed research tools, including a high efficiency of gene targeting and substantial genomics resources. However, current systems for controlled tran...

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Autores principales:	Minoru Kubo, Akihiro Imai, Tomoaki Nishiyama, Masaki Ishikawa, Yoshikatsu Sato, Tetsuya Kurata, Yuji Hiwatashi, Ralf Reski, Mitsuyasu Hasebe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/979b4ce35d3c40e3a4828db9295817f0
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spelling	oai:doaj.org-article:979b4ce35d3c40e3a4828db9295817f02021-11-18T08:53:34ZSystem for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.1932-620310.1371/journal.pone.0077356https://doaj.org/article/979b4ce35d3c40e3a4828db9295817f02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086772/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Inducible transgene expression provides a useful tool to analyze gene function. The moss Physcomitrellapatens is a model basal land plant with well-developed research tools, including a high efficiency of gene targeting and substantial genomics resources. However, current systems for controlled transgene expression remain limited. Here we report the development of an estrogen receptor mediated inducible gene expression system, based on the system used in flowering plants. After identifying the appropriate promoters to drive the chimeric transducer, we succeeded in inducing transcription over 1,000-fold after 24 h incubation with β-estradiol. The P. patens system was also effective for high-level long-term induction of gene expression; transcript levels of the activated gene were maintained for at least seven days on medium containing β-estradiol. We also established two potentially neutral targeting sites and a set of vectors for reproducible expression of two transgenes. This β-estradiol-dependent system will be useful to test genes individually or in combination, allowing stable, inducible transgenic expression in P. patens.Minoru KuboAkihiro ImaiTomoaki NishiyamaMasaki IshikawaYoshikatsu SatoTetsuya KurataYuji HiwatashiRalf ReskiMitsuyasu HasebePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e77356 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Minoru Kubo Akihiro Imai Tomoaki Nishiyama Masaki Ishikawa Yoshikatsu Sato Tetsuya Kurata Yuji Hiwatashi Ralf Reski Mitsuyasu Hasebe System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
description	Inducible transgene expression provides a useful tool to analyze gene function. The moss Physcomitrellapatens is a model basal land plant with well-developed research tools, including a high efficiency of gene targeting and substantial genomics resources. However, current systems for controlled transgene expression remain limited. Here we report the development of an estrogen receptor mediated inducible gene expression system, based on the system used in flowering plants. After identifying the appropriate promoters to drive the chimeric transducer, we succeeded in inducing transcription over 1,000-fold after 24 h incubation with β-estradiol. The P. patens system was also effective for high-level long-term induction of gene expression; transcript levels of the activated gene were maintained for at least seven days on medium containing β-estradiol. We also established two potentially neutral targeting sites and a set of vectors for reproducible expression of two transgenes. This β-estradiol-dependent system will be useful to test genes individually or in combination, allowing stable, inducible transgenic expression in P. patens.
format	article
author	Minoru Kubo Akihiro Imai Tomoaki Nishiyama Masaki Ishikawa Yoshikatsu Sato Tetsuya Kurata Yuji Hiwatashi Ralf Reski Mitsuyasu Hasebe
author_facet	Minoru Kubo Akihiro Imai Tomoaki Nishiyama Masaki Ishikawa Yoshikatsu Sato Tetsuya Kurata Yuji Hiwatashi Ralf Reski Mitsuyasu Hasebe
author_sort	Minoru Kubo
title	System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
title_short	System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
title_full	System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
title_fullStr	System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
title_full_unstemmed	System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
title_sort	system for stable β-estradiol-inducible gene expression in the moss physcomitrella patens.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/979b4ce35d3c40e3a4828db9295817f0
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System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.

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