Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria

Abstract Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles that originate from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to magnetosomes. Magnetosome...

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Autores principales:	Jacques Werckmann, Jefferson Cypriano, Christopher T. Lefèvre, Kassiogé Dembelé, Ovidiu Ersen, Dennis A. Bazylinski, Ulysses Lins, Marcos Farina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/979c6baf6fc742369b6382545860e456
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spelling	oai:doaj.org-article:979c6baf6fc742369b6382545860e4562021-12-02T12:31:59ZLocalized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria10.1038/s41598-017-08994-92045-2322https://doaj.org/article/979c6baf6fc742369b6382545860e4562017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08994-9https://doaj.org/toc/2045-2322Abstract Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles that originate from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to magnetosomes. Magnetosome magnetite crystals nucleate and grow using iron transported inside the vesicle by specific proteins. Here we address the question: can iron transported inside MTB for the production of magnetite crystals be spatially mapped using electron microscopy? Cultured and uncultured MTB from brackish and freshwater lagoons were studied using analytical transmission electron microscopy in an attempt to answer this question. Scanning transmission electron microscopy was used at sub-nanometric resolution to determine the distribution of elements by implementing high sensitivity energy dispersive X-ray (EDS) mapping and electron energy loss spectroscopy (EELS). EDS mapping showed that magnetosomes are enmeshed in a magnetosomal matrix in which iron accumulates close to the magnetosome forming a continuous layer visually appearing as a corona. EELS, obtained at high spatial resolution, confirmed that iron was present close to and inside the lipid bilayer magnetosome membrane. This study provides important clues to magnetite formation in MTB through the discovery of a mechanism where iron ions accumulate prior to magnetite biomineralization.Jacques WerckmannJefferson CyprianoChristopher T. LefèvreKassiogé DembeléOvidiu ErsenDennis A. BazylinskiUlysses LinsMarcos FarinaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jacques Werckmann Jefferson Cypriano Christopher T. Lefèvre Kassiogé Dembelé Ovidiu Ersen Dennis A. Bazylinski Ulysses Lins Marcos Farina Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
description	Abstract Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles that originate from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to magnetosomes. Magnetosome magnetite crystals nucleate and grow using iron transported inside the vesicle by specific proteins. Here we address the question: can iron transported inside MTB for the production of magnetite crystals be spatially mapped using electron microscopy? Cultured and uncultured MTB from brackish and freshwater lagoons were studied using analytical transmission electron microscopy in an attempt to answer this question. Scanning transmission electron microscopy was used at sub-nanometric resolution to determine the distribution of elements by implementing high sensitivity energy dispersive X-ray (EDS) mapping and electron energy loss spectroscopy (EELS). EDS mapping showed that magnetosomes are enmeshed in a magnetosomal matrix in which iron accumulates close to the magnetosome forming a continuous layer visually appearing as a corona. EELS, obtained at high spatial resolution, confirmed that iron was present close to and inside the lipid bilayer magnetosome membrane. This study provides important clues to magnetite formation in MTB through the discovery of a mechanism where iron ions accumulate prior to magnetite biomineralization.
format	article
author	Jacques Werckmann Jefferson Cypriano Christopher T. Lefèvre Kassiogé Dembelé Ovidiu Ersen Dennis A. Bazylinski Ulysses Lins Marcos Farina
author_facet	Jacques Werckmann Jefferson Cypriano Christopher T. Lefèvre Kassiogé Dembelé Ovidiu Ersen Dennis A. Bazylinski Ulysses Lins Marcos Farina
author_sort	Jacques Werckmann
title	Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
title_short	Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
title_full	Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
title_fullStr	Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
title_full_unstemmed	Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
title_sort	localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/979c6baf6fc742369b6382545860e456
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_version_	1718394229996126208

Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria

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