Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes.
Potato is the world's fourth largest food crop yet it continues to endure late blight, a devastating disease caused by the Irish famine pathogen Phytophthora infestans. Breeding broad-spectrum disease resistance (R) genes into potato (Solanum tuberosum) is the best strategy for genetically mana...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2008
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/979dd5358787488c8374dbb2bc747dcf |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:979dd5358787488c8374dbb2bc747dcf |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:979dd5358787488c8374dbb2bc747dcf2021-11-25T06:11:16ZEffector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes.1932-620310.1371/journal.pone.0002875https://doaj.org/article/979dd5358787488c8374dbb2bc747dcf2008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18682852/?tool=EBIhttps://doaj.org/toc/1932-6203Potato is the world's fourth largest food crop yet it continues to endure late blight, a devastating disease caused by the Irish famine pathogen Phytophthora infestans. Breeding broad-spectrum disease resistance (R) genes into potato (Solanum tuberosum) is the best strategy for genetically managing late blight but current approaches are slow and inefficient. We used a repertoire of effector genes predicted computationally from the P. infestans genome to accelerate the identification, functional characterization, and cloning of potentially broad-spectrum R genes. An initial set of 54 effectors containing a signal peptide and a RXLR motif was profiled for activation of innate immunity (avirulence or Avr activity) on wild Solanum species and tentative Avr candidates were identified. The RXLR effector family IpiO induced hypersensitive responses (HR) in S. stoloniferum, S. papita and the more distantly related S. bulbocastanum, the source of the R gene Rpi-blb1. Genetic studies with S. stoloniferum showed cosegregation of resistance to P. infestans and response to IpiO. Transient co-expression of IpiO with Rpi-blb1 in a heterologous Nicotiana benthamiana system identified IpiO as Avr-blb1. A candidate gene approach led to the rapid cloning of S. stoloniferum Rpi-sto1 and S. papita Rpi-pta1, which are functionally equivalent to Rpi-blb1. Our findings indicate that effector genomics enables discovery and functional profiling of late blight R genes and Avr genes at an unprecedented rate and promises to accelerate the engineering of late blight resistant potato varieties.Vivianne G A A VleeshouwersHendrik RietmanPavel KrenekNicolas ChampouretCarolyn YoungSang-Keun OhMiqia WangKlaas BouwmeesterBen VosmanRichard G F VisserEvert JacobsenFrancine GoversSophien KamounEdwin A G Van der VossenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 8, p e2875 (2008) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Vivianne G A A Vleeshouwers Hendrik Rietman Pavel Krenek Nicolas Champouret Carolyn Young Sang-Keun Oh Miqia Wang Klaas Bouwmeester Ben Vosman Richard G F Visser Evert Jacobsen Francine Govers Sophien Kamoun Edwin A G Van der Vossen Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| description |
Potato is the world's fourth largest food crop yet it continues to endure late blight, a devastating disease caused by the Irish famine pathogen Phytophthora infestans. Breeding broad-spectrum disease resistance (R) genes into potato (Solanum tuberosum) is the best strategy for genetically managing late blight but current approaches are slow and inefficient. We used a repertoire of effector genes predicted computationally from the P. infestans genome to accelerate the identification, functional characterization, and cloning of potentially broad-spectrum R genes. An initial set of 54 effectors containing a signal peptide and a RXLR motif was profiled for activation of innate immunity (avirulence or Avr activity) on wild Solanum species and tentative Avr candidates were identified. The RXLR effector family IpiO induced hypersensitive responses (HR) in S. stoloniferum, S. papita and the more distantly related S. bulbocastanum, the source of the R gene Rpi-blb1. Genetic studies with S. stoloniferum showed cosegregation of resistance to P. infestans and response to IpiO. Transient co-expression of IpiO with Rpi-blb1 in a heterologous Nicotiana benthamiana system identified IpiO as Avr-blb1. A candidate gene approach led to the rapid cloning of S. stoloniferum Rpi-sto1 and S. papita Rpi-pta1, which are functionally equivalent to Rpi-blb1. Our findings indicate that effector genomics enables discovery and functional profiling of late blight R genes and Avr genes at an unprecedented rate and promises to accelerate the engineering of late blight resistant potato varieties. |
| format |
article |
| author |
Vivianne G A A Vleeshouwers Hendrik Rietman Pavel Krenek Nicolas Champouret Carolyn Young Sang-Keun Oh Miqia Wang Klaas Bouwmeester Ben Vosman Richard G F Visser Evert Jacobsen Francine Govers Sophien Kamoun Edwin A G Van der Vossen |
| author_facet |
Vivianne G A A Vleeshouwers Hendrik Rietman Pavel Krenek Nicolas Champouret Carolyn Young Sang-Keun Oh Miqia Wang Klaas Bouwmeester Ben Vosman Richard G F Visser Evert Jacobsen Francine Govers Sophien Kamoun Edwin A G Van der Vossen |
| author_sort |
Vivianne G A A Vleeshouwers |
| title |
Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| title_short |
Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| title_full |
Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| title_fullStr |
Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| title_full_unstemmed |
Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| title_sort |
effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2008 |
| url |
https://doaj.org/article/979dd5358787488c8374dbb2bc747dcf |
| work_keys_str_mv |
AT viviannegaavleeshouwers effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT hendrikrietman effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT pavelkrenek effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT nicolaschampouret effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT carolynyoung effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT sangkeunoh effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT miqiawang effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT klaasbouwmeester effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT benvosman effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT richardgfvisser effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT evertjacobsen effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT francinegovers effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT sophienkamoun effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes AT edwinagvandervossen effectorgenomicsacceleratesdiscoveryandfunctionalprofilingofpotatodiseaseresistanceandphytophthorainfestansavirulencegenes |
| _version_ |
1718414058132078592 |