Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation

Abstract Systemic inflammation is a major feature of the post-cardiac arrest syndrome. The three monocyte subpopulations are thought to play an important role in this inflammatory state because they are endowed with numerous pattern recognition receptors, such as CD14, that have been associated with...

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Autores principales:	Alexander Asmussen, Hans-Jörg Busch, Thomas Helbing, Xavier Bemtgen, Christian Smolka, Christoph Bode, Katrin Fink, Sebastian Grundmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97a10b459da745539505269f54a3dfdd
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spelling	oai:doaj.org-article:97a10b459da745539505269f54a3dfdd2021-12-02T14:58:20ZMonocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation10.1038/s41598-021-91948-z2045-2322https://doaj.org/article/97a10b459da745539505269f54a3dfdd2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91948-zhttps://doaj.org/toc/2045-2322Abstract Systemic inflammation is a major feature of the post-cardiac arrest syndrome. The three monocyte subpopulations are thought to play an important role in this inflammatory state because they are endowed with numerous pattern recognition receptors, such as CD14, that have been associated with ischemia–reperfusion injury. By contrast, an exaggerated antiinflammatory response has also been described following cardiac arrest, which may be mediated by downregulation of antigen presentation receptor HLA-DR. We report the composition of monocyte subpopulations and the expression of CD14 and HLA-DR following cardiac arrest. Blood specimens were collected from 32 patients at three timepoints in the first 48 h after cardiac arrest. Monocyte subset composition was determined by flow cytometry based on the expression of CD14, CD16, and HLA-DR. Monocyte subset composition and the expression of CD14 and HLA-DR were correlated with patient outcomes. The results were compared to 19 patients with coronary artery disease. Cardiac arrest patients showed a significant decline in the percentage of nonclassical monocytes. Monocyte CD14 expression was upregulated after 24 h and correlated with the time to return of spontaneous circulation. Downregulation of HLA-DR expression was observed mainly among classical monocytes and significantly correlated with the dose of norepinephrine used to treat shock. Downregulation of HLA-DR among nonclassical and intermediate monocytes was significantly associated with disease severity. Our data demonstrate the disturbance of monocyte subset composition with a significant decline in nonclassical monocytes at an early stage following cardiac arrest. Our findings suggest the simultaneous presence of hyperinflammation, as evidenced by upregulation of CD14, and monocyte deactivation, characterized by downregulation of HLA-DR. The extent of monocyte deactivation was significantly correlated with disease severity.Alexander AsmussenHans-Jörg BuschThomas HelbingXavier BemtgenChristian SmolkaChristoph BodeKatrin FinkSebastian GrundmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander Asmussen Hans-Jörg Busch Thomas Helbing Xavier Bemtgen Christian Smolka Christoph Bode Katrin Fink Sebastian Grundmann Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation
description	Abstract Systemic inflammation is a major feature of the post-cardiac arrest syndrome. The three monocyte subpopulations are thought to play an important role in this inflammatory state because they are endowed with numerous pattern recognition receptors, such as CD14, that have been associated with ischemia–reperfusion injury. By contrast, an exaggerated antiinflammatory response has also been described following cardiac arrest, which may be mediated by downregulation of antigen presentation receptor HLA-DR. We report the composition of monocyte subpopulations and the expression of CD14 and HLA-DR following cardiac arrest. Blood specimens were collected from 32 patients at three timepoints in the first 48 h after cardiac arrest. Monocyte subset composition was determined by flow cytometry based on the expression of CD14, CD16, and HLA-DR. Monocyte subset composition and the expression of CD14 and HLA-DR were correlated with patient outcomes. The results were compared to 19 patients with coronary artery disease. Cardiac arrest patients showed a significant decline in the percentage of nonclassical monocytes. Monocyte CD14 expression was upregulated after 24 h and correlated with the time to return of spontaneous circulation. Downregulation of HLA-DR expression was observed mainly among classical monocytes and significantly correlated with the dose of norepinephrine used to treat shock. Downregulation of HLA-DR among nonclassical and intermediate monocytes was significantly associated with disease severity. Our data demonstrate the disturbance of monocyte subset composition with a significant decline in nonclassical monocytes at an early stage following cardiac arrest. Our findings suggest the simultaneous presence of hyperinflammation, as evidenced by upregulation of CD14, and monocyte deactivation, characterized by downregulation of HLA-DR. The extent of monocyte deactivation was significantly correlated with disease severity.
format	article
author	Alexander Asmussen Hans-Jörg Busch Thomas Helbing Xavier Bemtgen Christian Smolka Christoph Bode Katrin Fink Sebastian Grundmann
author_facet	Alexander Asmussen Hans-Jörg Busch Thomas Helbing Xavier Bemtgen Christian Smolka Christoph Bode Katrin Fink Sebastian Grundmann
author_sort	Alexander Asmussen
title	Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation
title_short	Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation
title_full	Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation
title_fullStr	Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation
title_full_unstemmed	Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation
title_sort	monocyte subset distribution and surface expression of hla-dr and cd14 in patients after cardiopulmonary resuscitation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/97a10b459da745539505269f54a3dfdd
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Monocyte subset distribution and surface expression of HLA-DR and CD14 in patients after cardiopulmonary resuscitation

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