In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.

<h4>Objectives</h4>The in vivo efficacy of a cefotaxime-ciprofloxacin combination against Vibrio vulnificus and the effects on rtxA1 expression of commonly used antibiotics are unknown.<h4>Methods</h4>In vitro time-kill studies were performed to evaluate synergism. Female BAL...

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Autores principales:	Hee-Chang Jang, Su-Mi Choi, Hee Kyung Kim, Sung-Eun Kim, Seung-Ji Kang, Kyung-Hwa Park, Phil Youl Ryu, Tae-Hoon Lee, Young Ran Kim, Joon Haeng Rhee, Sook-In Jung, Hyon E Choy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97a95066ab3e4ce8b0039230fa9e10cd
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spelling	oai:doaj.org-article:97a95066ab3e4ce8b0039230fa9e10cd2021-11-25T06:10:21ZIn vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.1932-620310.1371/journal.pone.0101118https://doaj.org/article/97a95066ab3e4ce8b0039230fa9e10cd2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24978586/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objectives</h4>The in vivo efficacy of a cefotaxime-ciprofloxacin combination against Vibrio vulnificus and the effects on rtxA1 expression of commonly used antibiotics are unknown.<h4>Methods</h4>In vitro time-kill studies were performed to evaluate synergism. Female BALB/c mice were injected subcutaneously with 1×10(7) or 1×10(8) cfu of V. vulnificus. Antibiotic therapy was initiated at 2 h after inoculation in the following four therapy groups: cefotaxime; ciprofloxacin; cefotaxime-plus-ciprofloxacin; and cefotaxime-plus-minocycline. The cytotoxicity of V. vulnificus for HeLa cells was measured using the lactate dehydrogenase assay; rtxA1 transcription was measured in a transcriptional reporter strain using a β-galactosidase assay.<h4>Results</h4>In vitro time-kill assays exhibited synergism between cefotaxime and ciprofloxacin. In the animal experiments, the 96-h survival rate for the cefotaxime-plus-ciprofloxacin group (85%; 17/20) was significantly higher than that of the cefotaxime-plus-minocycline (35%; 7/20) and cefotaxime alone (0%; 0/20) groups (P<0.05 for both). Bacterial counts in the liver and spleen were significantly lower in the cefotaxime-plus-ciprofloxacin group 24 and 48 h after treatment, relative to the other groups. At sub-inhibitory concentrations, ciprofloxacin inhibited more effectively rtxA1 transcription and mammalian cell cytotoxicity than either minocycline or cefotaxime (P<0.05 for both).<h4>Conclusions</h4>Ciprofloxacin is more effective at reducing rtxA1 transcription and subsequent cytotoxicity than either minocycline or cefotaxime, and the combination of ciprofloxacin and cefotaxime was more effective in clearing V. vulnificus in vivo than previously used regimens. These data suggest that the combination of ciprofloxacin and cefotaxime is an effective option for the treatment of V. vulnificus sepsis in humans.Hee-Chang JangSu-Mi ChoiHee Kyung KimSung-Eun KimSeung-Ji KangKyung-Hwa ParkPhil Youl RyuTae-Hoon LeeYoung Ran KimJoon Haeng RheeSook-In JungHyon E ChoyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e101118 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hee-Chang Jang Su-Mi Choi Hee Kyung Kim Sung-Eun Kim Seung-Ji Kang Kyung-Hwa Park Phil Youl Ryu Tae-Hoon Lee Young Ran Kim Joon Haeng Rhee Sook-In Jung Hyon E Choy In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.
description	<h4>Objectives</h4>The in vivo efficacy of a cefotaxime-ciprofloxacin combination against Vibrio vulnificus and the effects on rtxA1 expression of commonly used antibiotics are unknown.<h4>Methods</h4>In vitro time-kill studies were performed to evaluate synergism. Female BALB/c mice were injected subcutaneously with 1×10(7) or 1×10(8) cfu of V. vulnificus. Antibiotic therapy was initiated at 2 h after inoculation in the following four therapy groups: cefotaxime; ciprofloxacin; cefotaxime-plus-ciprofloxacin; and cefotaxime-plus-minocycline. The cytotoxicity of V. vulnificus for HeLa cells was measured using the lactate dehydrogenase assay; rtxA1 transcription was measured in a transcriptional reporter strain using a β-galactosidase assay.<h4>Results</h4>In vitro time-kill assays exhibited synergism between cefotaxime and ciprofloxacin. In the animal experiments, the 96-h survival rate for the cefotaxime-plus-ciprofloxacin group (85%; 17/20) was significantly higher than that of the cefotaxime-plus-minocycline (35%; 7/20) and cefotaxime alone (0%; 0/20) groups (P<0.05 for both). Bacterial counts in the liver and spleen were significantly lower in the cefotaxime-plus-ciprofloxacin group 24 and 48 h after treatment, relative to the other groups. At sub-inhibitory concentrations, ciprofloxacin inhibited more effectively rtxA1 transcription and mammalian cell cytotoxicity than either minocycline or cefotaxime (P<0.05 for both).<h4>Conclusions</h4>Ciprofloxacin is more effective at reducing rtxA1 transcription and subsequent cytotoxicity than either minocycline or cefotaxime, and the combination of ciprofloxacin and cefotaxime was more effective in clearing V. vulnificus in vivo than previously used regimens. These data suggest that the combination of ciprofloxacin and cefotaxime is an effective option for the treatment of V. vulnificus sepsis in humans.
format	article
author	Hee-Chang Jang Su-Mi Choi Hee Kyung Kim Sung-Eun Kim Seung-Ji Kang Kyung-Hwa Park Phil Youl Ryu Tae-Hoon Lee Young Ran Kim Joon Haeng Rhee Sook-In Jung Hyon E Choy
author_facet	Hee-Chang Jang Su-Mi Choi Hee Kyung Kim Sung-Eun Kim Seung-Ji Kang Kyung-Hwa Park Phil Youl Ryu Tae-Hoon Lee Young Ran Kim Joon Haeng Rhee Sook-In Jung Hyon E Choy
author_sort	Hee-Chang Jang
title	In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.
title_short	In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.
title_full	In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.
title_fullStr	In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.
title_full_unstemmed	In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.
title_sort	in vivo efficacy of the combination of ciprofloxacin and cefotaxime against vibrio vulnificus sepsis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/97a95066ab3e4ce8b0039230fa9e10cd
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In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis.

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