A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.

Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fo...

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Autores principales:	Akos Gellért, Katalin Salánki, Kata Tombácz, Tamás Tuboly, Ervin Balázs
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97a9554cb8a345f088d301a0a9636665
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spelling	oai:doaj.org-article:97a9554cb8a345f088d301a0a96366652021-11-18T08:04:04ZA cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.1932-620310.1371/journal.pone.0052688https://doaj.org/article/97a9554cb8a345f088d301a0a96366652012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285149/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines.Akos GellértKatalin SalánkiKata TombáczTamás TubolyErvin BalázsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52688 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akos Gellért Katalin Salánki Kata Tombácz Tamás Tuboly Ervin Balázs A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
description	Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines.
format	article
author	Akos Gellért Katalin Salánki Kata Tombácz Tamás Tuboly Ervin Balázs
author_facet	Akos Gellért Katalin Salánki Kata Tombácz Tamás Tuboly Ervin Balázs
author_sort	Akos Gellért
title	A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
title_short	A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
title_full	A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
title_fullStr	A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
title_full_unstemmed	A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
title_sort	cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/97a9554cb8a345f088d301a0a9636665
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A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.

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