Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells

Abstract There is an unmet need for novel, non-pharmacological therapeutics to treat alopecia. Recent studies have shown the potential biological benefits of non-thermal atmospheric pressure plasma (NTAPP), including wound healing, angiogenesis, and the proliferation of stem cells. We hypothesized t...

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Autores principales: Ji-Hye Hwang, Hyun-Young Lee, Kyung Bae Chung, Hae June Lee, Jino Kim, Kiwon Song, Do-Young Kim
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/97ada8a8dcd4426ca96a7567b64fcd22
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spelling oai:doaj.org-article:97ada8a8dcd4426ca96a7567b64fcd222021-12-02T18:50:49ZNon-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells10.1038/s41598-021-95650-y2045-2322https://doaj.org/article/97ada8a8dcd4426ca96a7567b64fcd222021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95650-yhttps://doaj.org/toc/2045-2322Abstract There is an unmet need for novel, non-pharmacological therapeutics to treat alopecia. Recent studies have shown the potential biological benefits of non-thermal atmospheric pressure plasma (NTAPP), including wound healing, angiogenesis, and the proliferation of stem cells. We hypothesized that NTAPP might have a stimulatory effect on hair growth or regeneration. We designed an NTAPP-generating apparatus which is applicable to in vitro and in vivo experiments. The human dermal papilla (DP) cells, isolated fresh hair follicles, and mouse back skin were exposed with the NTAPP. Biological outcomes were measured using RNA-sequencing, RT-PCR, Western blots, and immunostaining. The NTAPP treatment increased the expression levels of Wnt/β-catenin pathway-related genes (AMER3, CCND1, LEF1, and LRG1) and proteins (β-catenin, p-GSK3β, and cyclin D1) in human DP cells. In contrast, inhibitors of Wnt/β-catenin signaling, endo-IWR1 and IWP2, attenuated the levels of cyclin D1, p-GSK3β, and β-catenin proteins induced by NTAPP. Furthermore, we observed that NTAPP induced the activation of β-catenin in DP cells of hair follicles and the mRNA levels of target genes of the β-catenin signaling pathway (CCND1, LEF1, and TCF4). NTAPP-treated mice exhibited markedly increased anagen induction, hair growth, and the protein levels of β-catenin, p-GSK3β, p-AKT, and cyclin D1. NTAPP stimulates hair growth via activation of the Wnt/β-catenin signaling pathway in DP cells. These findings collectively suggest that NTAPP may be a potentially safe and non-pharmacological therapeutic intervention for alopecia.Ji-Hye HwangHyun-Young LeeKyung Bae ChungHae June LeeJino KimKiwon SongDo-Young KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ji-Hye Hwang
Hyun-Young Lee
Kyung Bae Chung
Hae June Lee
Jino Kim
Kiwon Song
Do-Young Kim
Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells
description Abstract There is an unmet need for novel, non-pharmacological therapeutics to treat alopecia. Recent studies have shown the potential biological benefits of non-thermal atmospheric pressure plasma (NTAPP), including wound healing, angiogenesis, and the proliferation of stem cells. We hypothesized that NTAPP might have a stimulatory effect on hair growth or regeneration. We designed an NTAPP-generating apparatus which is applicable to in vitro and in vivo experiments. The human dermal papilla (DP) cells, isolated fresh hair follicles, and mouse back skin were exposed with the NTAPP. Biological outcomes were measured using RNA-sequencing, RT-PCR, Western blots, and immunostaining. The NTAPP treatment increased the expression levels of Wnt/β-catenin pathway-related genes (AMER3, CCND1, LEF1, and LRG1) and proteins (β-catenin, p-GSK3β, and cyclin D1) in human DP cells. In contrast, inhibitors of Wnt/β-catenin signaling, endo-IWR1 and IWP2, attenuated the levels of cyclin D1, p-GSK3β, and β-catenin proteins induced by NTAPP. Furthermore, we observed that NTAPP induced the activation of β-catenin in DP cells of hair follicles and the mRNA levels of target genes of the β-catenin signaling pathway (CCND1, LEF1, and TCF4). NTAPP-treated mice exhibited markedly increased anagen induction, hair growth, and the protein levels of β-catenin, p-GSK3β, p-AKT, and cyclin D1. NTAPP stimulates hair growth via activation of the Wnt/β-catenin signaling pathway in DP cells. These findings collectively suggest that NTAPP may be a potentially safe and non-pharmacological therapeutic intervention for alopecia.
format article
author Ji-Hye Hwang
Hyun-Young Lee
Kyung Bae Chung
Hae June Lee
Jino Kim
Kiwon Song
Do-Young Kim
author_facet Ji-Hye Hwang
Hyun-Young Lee
Kyung Bae Chung
Hae June Lee
Jino Kim
Kiwon Song
Do-Young Kim
author_sort Ji-Hye Hwang
title Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells
title_short Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells
title_full Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells
title_fullStr Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells
title_full_unstemmed Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells
title_sort non-thermal atmospheric pressure plasma activates wnt/β-catenin signaling in dermal papilla cells
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/97ada8a8dcd4426ca96a7567b64fcd22
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